The use of low-level light therapy in supportive care for patients with breast cancer: review of the literature

Robijns, Jolien; Censabella, Sandrine; Bulens, Paul; Maes, André; Mebis, Jeroen

doi:10.1007/s10103-016-2056-y

Cited by 56 publications

(46 citation statements)

References 86 publications

(102 reference statements)

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“…In the case of orthodontic pain, a systematic review showed positive effects of PBM in reducing intense pain since the first day of treatment . PBM has also been demonstrated to be an effective supportive therapy for the management of several side effects caused by breast cancer therapies, including radiodermatitis, lymphedema, chemotherapy‐induced peripheral neuropathy, osteonecrosis of the jaw and oral mucositis in paediatric patients, which all include a pain component .…”

Section: Introductionmentioning

confidence: 99%

Analgesic effect of Photobiomodulation Therapy: An in vitro and in vivo study

Zupin

Ottaviani

Rupel

et al. 2019

Journal of Biophotonics

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Laser therapy, also known as Photobiomodulation (PBM) is indicated to reduce pain associated with different pathologies and applied using protocols that vary in wavelength, irradiance and fluence. Its mechanisms of action are still unclear and possibly able to directly impact on pain transmission, reducing nociceptor response. In our study, we examined the effect of two specific laser wavelengths, 800 and 970 nm, extensively applied in the clinical context and known to exert important analgesic effects. Our results point to mitochondria as the primary target of laser light in isolated dorsal root ganglion (DRG) neurons, reducing adenosine triphosphate content and increasing reactive oxygen species levels. Specifically, the 800 nm laser wavelength induced mitochondrial dysregulation, that is, increased superoxide generation and mitochondrial membrane potential. When DRG neurons were firstly illuminated by the different laser protocols and then stimulated with the natural transient receptor potential cation channel subfamily V member 1 (TRPV1) ligand capsaicin, only the 970 nm wavelength reduced the calcium response, in both amplitude and frequency. Consistent results were obtained in vivo in mice, by subcutaneous injection of capsaicin. Our findings demonstrate that the effect of PBM depends on the wavelength used, with 800 nm light mainly acting on mitochondrial metabolism and 970 nm light on nociceptive signal transmission.

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Section: Introductionmentioning

confidence: 99%

Analgesic effect of Photobiomodulation Therapy: An in vitro and in vivo study

Zupin

Ottaviani

Rupel

et al. 2019

Journal of Biophotonics

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“…Low-level laser therapy (LLLT) is being studied as a non-invasive treatment option for ORM (3, 5, 6). Initial clinical studies show promising results in the reduction of pain and improvement of the quality of life after LLLT treatment (7–12).…”

Section: Introductionmentioning

confidence: 99%

Low-Level Laser Therapy Stimulates Proliferation in Head and Neck Squamous Cell Carcinoma Cells

2018

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Objectives: Low-level laser therapy (LLLT) is a promising non-invasive treatment option for oropharyngeal mucositis, which is a common side effect of many oncological treatments. LLLT is known for its wound healing properties due to the stimulation of cellular processes, such as proliferation, migration and differentiation. Controversy exists on the possible stimulatory effect of LLLT on head and neck cancer (HNSCC) cells in patients treated with radiotherapy. The aim of this study was to evaluate the biostimulatory effect together with the underlying mechanisms of LLLT on HNSCC cancer cells and normal epithelial cells.Materials and methods: HNSCC cell lines (SCC154, SQD9, and SCC61) and human tonsil epithelial cells were exposed to a Gallium-Aluminum-Arsenide diode laser (830 nm, 150 mW) with energy densities of 0, 1, and 2 J/cm2. The proliferation potential of the cells was assessed by Sulforhodamine B assay, immunoblotting (mitogenic pathways), immunocytochemistry (Ki67), and flow cytometry (PI cell cycle staining).Results: Cell proliferation was increased in HNSCC cell lines after laser irradiation with 1 J/cm2, whereas no significant increase was seen after laser irradiation with 2 J/cm2. In contrast, no effect on cell proliferation was seen in the human tonsil epithelial cells after laser irradiation with any of the energy densities. The increased proliferation was associated with elevated levels of pAKT, pERK, and Ki67 protein expression and cell cycle progression.Conclusion: Our results show that LLLT increases cell proliferation in a dose-dependent manner in HNSCC cells but not in normal epithelial tonsil cells. These results suggest that LLLT has to be used with caution when treating oropharyngeal mucositis in HNSCC patients since tumor cells present in the LLLT irradiation field could be triggered by LLLT.

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“…Prevention, early detection, and effective treatment are the key intervention points to increase the survival rate (1,2). About 1.7 million new breast cancer patients were diagnosed in 2012, representing approximately 12% of all cancer cases globally that year (3). Currently, breast cancer is classified into five major groups on the basis of molecular profiling.…”

Section: Introductionmentioning

confidence: 99%

Triptolide induces autophagy and apoptosis through ERK activation in human breast cancer MCF‑7 cells

et al. 2018

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Abstract.To investigate the effects of triptolide (TPI) on proliferation, autophagy and death in human breast cancer MCF-7 cells, and to elucidate the associated molecular mechanisms, intracellular alterations were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays. The results of the MTT assay revealed that TPI significantly reduced the MCF-7 cell survival rate when the concentration was >10 nmol/l. TPI activated a caspase cascade reaction by regulating Bcl-2-associated X protein (Bax), caspase-3 and B-cell lymphoma 2 expression, and promoted programmed cell death via the mitochondrial pathway. The results demonstrated that TPI significantly reduced the cell proliferation rate and viability in a time-and dose-dependent manner, which was confirmed by western blotting and immunofluorescent staining. TPI induced autophagy and influenced p38 mitogen-activated protein kinases, extracellular signal-regulated kinase (Erk)1/2, and mammalian target of rapamycin (mTOR) phosphorylation, which resulted in apoptosis. When cells were treated with a combination of TPI and the Erk1/2 inhibitor U0126, the downregulation of P62 and upregulation of Bax were inhibited, which demonstrated that the inhibition of Erk1/2 reversed the autophagy changes induced by TPI. The results indicated that Erk1/2 activation may be a novel mechanism by which TPI induces autophagy and apoptosis in MCF-7 breast cancer cells. In conclusion, TPI affects the proliferation and apoptosis of MCF-7 cells, potentially via autophagy and p38/Erk/mTOR phosphorylation. The present study offers a novel view of the mechanisms by which TPI regulates cell death.

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The use of low-level light therapy in supportive care for patients with breast cancer: review of the literature

Cited by 56 publications

References 86 publications

Analgesic effect of Photobiomodulation Therapy: An in vitro and in vivo study

Analgesic effect of Photobiomodulation Therapy: An in vitro and in vivo study

Low-Level Laser Therapy Stimulates Proliferation in Head and Neck Squamous Cell Carcinoma Cells

Triptolide induces autophagy and apoptosis through ERK activation in human breast cancer MCF‑7 cells

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