Reclassification of the larval pathogen for marine bivalves Vibrio tubiashii subsp. europaeus as Vibrio europaeus sp. nov.

Dubert, Javier; Romalde, Jesús L.; Spinard, Edward; Nelson, David R.; Gomez‐Chiarri, Marta; Barja, Juan L.

doi:10.1099/ijsem.0.001431

Cited by 17 publications

(15 citation statements)

References 26 publications

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“…24 and Dubert et al . 25 where they demonstrated the lack of agreement between ANIb and ANIm when the compared genomes show low correlation values (ANIb < 75% and <77%, respectively), in our case the ANIb value is <79%.…”

Section: Resultscontrasting

confidence: 56%

PirVP genes causing AHPND identified in a new Vibrio species (Vibrio punensis) within the commensal Orientalis clade

Restrepo

Bayot

Arciniegas

et al. 2018

Sci Rep

110

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Acute hepatopancreatic necrosis disease (AHPND) has extended rapidly, causing alarming shrimp mortalities. Initially, the only known causative agent was Vibrio parahaemolyticus carrying a plasmid coding for the mortal toxins PirVP. Recently, it has been found that the plasmid and hence the disease, could be transferred among members of the Harveyi clade. The current study performs a genomic characterization of an isolate capable of developing AHPND in shrimp. Mortality studies and molecular and histopathological analyses showed the infection capacity of the strain. Multilocus sequence analysis placed the bacteria as a member of the Orientalis clade, well known for containing commensal and even probiotic bacteria used in the shrimp industry. Further whole genome comparative analyses, including Vibrio species from the Orientalis clade, and phylogenomic metrics (TETRA, ANI and DDH) showed that the isolate belongs to a previously unidentified species, now named Vibrio punensis sp. nov. strain BA55. Our findings show that the gene transfer capacity of Vibrio species goes beyond the clade classification, demonstrating a new pathogenic capacity to a previously known commensal clade. The presence of these genes in a different Vibrio clade may contribute to the knowledge of the Vibrio pathogenesis and has major implications for the spread of emerging diseases.

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Section: Resultscontrasting

confidence: 56%

PirVP genes causing AHPND identified in a new Vibrio species (Vibrio punensis) within the commensal Orientalis clade

Restrepo

Bayot

Arciniegas

et al. 2018

Sci Rep

110

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show abstract

“…However, this study showed some taxonomic incongruities. Later, studies carried out by Dubert et al (2016e) employing a polyphasic approach that included the WGS analysis, as well as phenotypic tests and chemotaxonomic techniques, supported their elevation to the rank of species. Strains described in the initial study were isolated from O. edulis and R. philippinarum during disease outbreaks in different Spanish hatcheries (Prado et al, 2005, 2015).…”

Section: Overview Of the Pathogenic Vibrios For Bivalve Larvae And Spmentioning

confidence: 99%

“…Vibrio europaeus is the pathogen of bivalve larvae most recently described (Dubert et al, 2016e). Initially, this taxon was described as a subespecies of V. tubiashii , V. tubiashii subsp.…”

Section: Overview Of the Pathogenic Vibrios For Bivalve Larvae And Spmentioning

confidence: 99%

“…Strains described in the initial study were isolated from O. edulis and R. philippinarum during disease outbreaks in different Spanish hatcheries (Prado et al, 2005, 2015). Interestingly, Dubert et al (2016e) included in the description of the new taxa a French isolate initially identified as V. tubiashii , and highly pathogenic for C. gigas larvae and spat (Mersni-Achour et al, 2014, 2015; Travers et al, 2014). Recently, Dubert et al (2017) have reported a mortality event in larvae of other clam species, namely R. decussatus , involving this bacterial species.…”

Section: Overview Of the Pathogenic Vibrios For Bivalve Larvae And Spmentioning

confidence: 99%

“…Hence, term vibriosis is generally extended to refer the bacterial disease in bivalve larvae and spat caused by pathogenic Vibrio species (Brown and Losee, 1978; Elston and Leibovitz, 1980; Elston, 1999). Different Vibrio species have been described as the etiological agent responsible of vibriosis promoting the larval and spat mortalities of different hatchery cultured bivalve species worldwide (Tubiash et al, 1965; Jeffries, 1982; Lodeiros et al, 1987; Nicolas et al, 1996; Estes et al, 2004; Gómez-León et al, 2005; Prado et al, 2005; Elston et al, 2008; Kesarcodi-Watson et al, 2009; Travers et al, 2014; Richards et al, 2014b; Rojas et al, 2015; Dubert et al, 2016d,e). …”

Section: Introductionmentioning

confidence: 99%

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New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects

2017

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Hatcheries constitute nowadays the only viable solution to support the husbandry of bivalve molluscs due to the depletion and/or overexploitation of their natural beds. Hatchery activities include the broodstock conditioning and spawning, rearing larvae and spat, and the production of microalgae to feed all stages of the production cycle. However, outbreaks of disease continue to be the main bottleneck for successful larval and spat production, most of them caused by different representatives of the genus Vibrio. Therefore, attention must be paid on preventive and management measures that allow the control of such undesirable bacterial populations. The present review provides an updated picture of the recently characterized Vibrio species associated with disease of bivalve molluscs during early stages of development, including the controversial taxonomic affiliation of some of them and relevant advances in the knowledge of their virulence determinants. The problematic use of antibiotics, as well as its eco-friendly alternatives are also critically discussed.

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A MALDI-TOF MS database for fast identification of Vibrio spp. potentially pathogenic to marine mollusks

Moussa

Cauvin

Piouffle³

et al. 2021

Appl Microbiol Biotechnol

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In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify Vibrio species are unsuitable for monitoring programs because they are time-consuming and expensive. The aim of this study was, therefore, to develop the MALDI-TOF MS method in order to establish a rapid identification technique for a large panel of Vibrio species. We created the EnviBase containing 120 main spectra projections (MSP) of the Vibrio species that are potentially responsible for mollusk diseases, comprising 25 species: V. aestuarianus, V. cortegadensis, V. tapetis and species belonging to the Coralliilyticus, Harveyi, Mediterranei, and Orientalis clades. Each MSP was constructed by the merger of raw spectra obtained from three different media and generated by three collaborating laboratories to increase the diversity of the conditions and thus obtain a good technique robustness. Perfect discrimination was obtained with all of the MSP created for the Vibrio species and even for very closely related species as V. europaeus and V. bivalvicida. The new EnviBase library was validated through a blind test on 100 Vibrio strains performed by our three collaborators who used the direct transfer and protein extraction methods. The majority of the Vibrio strains were successfully identified with the newly created EnviBase by the three laboratories for both protocol methods. This study documents the first development of a freely accessible database exclusively devoted to Vibrio found in marine environments, taking into account the high diversity of this genus. Key points • Development of a MALDI-TOF MS database to quickly affiliate Vibrio species. • Increase of the reactivity when faced with Vibrio associated with mollusk diseases. • Validation of MALDI-TOF MS as routine diagnostic tool.

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Reclassification of the larval pathogen for marine bivalves Vibrio tubiashii subsp. europaeus as Vibrio europaeus sp. nov.

Cited by 17 publications

References 26 publications

PirVP genes causing AHPND identified in a new Vibrio species (Vibrio punensis) within the commensal Orientalis clade

PirVP genes causing AHPND identified in a new Vibrio species (Vibrio punensis) within the commensal Orientalis clade

New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects

A MALDI-TOF MS database for fast identification of Vibrio spp. potentially pathogenic to marine mollusks

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