Correlation betweenZBED6Gene Upstream CpG Island methylation and mRNA expression in cattle

Abstract

DNA methylation is essential for the regulation of gene expression and important roles in muscle development. To assess the extent of epigenetic modifications and gene expression on the differentially methylated region (DMR) in ZBED6, we simultaneously examined DNA methylation and expression in six tissues from two different developmental stages (fetal bovine and adult bovine). The DNA methylation pattern was compared using bisulfite sequencing polymerase chain reaction (BSP) and combined bisulfite restriction analysis (COBRA). The result of quantitative real-time PCR (qPCR) analysis showed that ZBED6 has a broad tissue distribution and is highly expressed in adult bovine (P < 0.05 or P < 0.01). The DNA methylation level was significantly different in liver, lung and spleen between the two cattle groups (P < 0.05 or P < 0.01). The adult bovine group exhibited a significantly higher mRNA level and lower DNA methylation level than the fetal bovine group in liver, lung, and spleen. No significant association was detected between DNA methylation level and muscle, heart, and kidney at two different stages. In this study, the statistical analyses indicated that DNA methylation patterns are associated with mRNA level in some tissues, these results may be a useful parameter to investigate muscle developmental in cattle and as a model for studies in other species, potentially contributing to an improvement of growth performance selection in beef cattle breeding program.

Transcriptional regulation of the bovine FGFR1 gene facilitates myoblast proliferation under hypomethylation of the promoter

“…Differential methylation can significantly affect the mRNA expression level of the FGFR1 gene. Our results are consistent with previous studies reporting that the higher the degree of methylation, the lower the expression of FGFR1 (Huang et al, 2017; Shi et al, 2018). Our results suggest that there is a DMR in the promoter region of FGFR1 , and that a higher degree of methylation is associated with lower expression of the FGFR1 gene at different developmental stages.…”

Unlocking the Transcriptional Control of NCAPG in Bovine Myoblasts: CREB1 and MYOD1 as Key Players

“…DNA methylation, an essential epigenetic modification, is indispensable for mammalian development and plays a pivotal role in the recognition and maintenance of cellular identity [ 38 ]. Generally, methylation levels typically impact mRNA expression [ 39 ]. Previous research has demonstrated that DNA methylation of the bovine Six1 gene promoter significantly influences promoter activity and exhibits a negative correlation with Six1 gene expression [ 40 ].…”