Shifts in Rumen Fermentation and Microbiota Are Associated with Dissolved Ruminal Hydrogen Concentrations in Lactating Dairy Cows Fed Different Types of Carbohydrates

Wang, Min; Wang, Rong; Xie, Tian; Janssen, Peter H.; Sun, Xuezhao; Beauchemin, K. A.; Tan, Zhi Liang; Gao, Min

doi:10.3945/jn.116.232462

Cited by 69 publications

(61 citation statements)

References 33 publications

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“…The interaction between DM intake and period in this study suggests that feeding CS may sometimes increase DM intake. In addition, a higher fiber content may stimulate microbial fibrolytic activity, further improving fiber digestibility (Wang et al 2016). The result was consistent with the conventional idea that 2 weeks of adaption are necessary to accurately measure a treatment effect and to minimize the potential confounding influence of carry-over from a previous treatment.…”

Section: Discussionsupporting

confidence: 85%

“…The possible reason can be that higher concentration of recalcitrant fiber increased the retention time in the rumen and reduced the mass flow to the lower tract, as explained in Kendall et al (2009) that total tract digestibility of NDF was greater for cows consuming 32% NDF compared with 28% NDF diets. In addition, a higher fiber content may stimulate microbial fibrolytic activity, further improving fiber digestibility (Wang et al 2016). Whether the fiber content ‡Sequence AC: dietary change from an alfalfa hay-(the day before transition) to a corn stover-based diet (the day after transition); sequence CA: dietary change from a corn stover-(the day before transition) to an alfalfa haybased diet (the day after transition).…”

Section: Discussionmentioning

confidence: 99%

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Effect of changing forage on the dynamic variation in rumen fermentation in sheep

Xie

Wang

Guan

et al. 2017

Animal Science Journal

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To better understand rumen adaptation during dietary transitions between high- and low-quality forages, 10 rumen-cannulated Hu sheep were randomly allocated to two dietary treatments (five sheep each) with the same concentrate-to-forage ratio and concentration mixture, but different forage sequences: (i) alfalfa hay (AH) to corn stover (CS) and back to AH; and (ii) CS to AH and back to CS. A significant decrease in the rumen microbial protein concentration was observed on day 6 after dietary transition whether the transition was from AH to CS or from CS to AH, and this was accompanied by an increase in the ammonia nitrogen concentration as well as a decrease in the total volatile fatty acids concentration and pH. However, after transitioning back to the original forage, the rumen fermentation parameters returned to their initial levels within 2 weeks. Our findings suggest that abrupt substitutions of forages with large nutrient differences could influence rumen function to some extent, but recovery can occur within 2 weeks without detrimental effects. Furthermore, we speculate that the variation of fermentation in the first 6 days may indicate an important rumen transition stage that requires further study.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Effect of changing forage on the dynamic variation in rumen fermentation in sheep

Xie

Wang

Guan

et al. 2017

Animal Science Journal

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“…For both the metabolite concentrations and the microbiota composition, time from feeding clearly explained more variation than diet. Differences in the degradation rates of various types of carbohydrates may explain the observed temporal variation in metabolite concentration (Leedle et al, 1982) and microbiota composition (e.g., Rooke et al, 2014; Li et al, 2015; Wang et al, 2016b). Therefore, an experimental approach with contrasts in carbohydrate degradation rate, rather than contrasts in the amount of dietary fat, might have revealed even more about the microbial metabolic dynamics in the rumen.…”

Section: Discussionmentioning

confidence: 99%

Diurnal Dynamics of Gaseous and Dissolved Metabolites and Microbiota Composition in the Bovine Rumen

et al. 2017

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Diurnal patterns of ruminal fermentation metabolites and microbial communities are not commonly assessed when investigating variation in ruminal CH4 production. The aims of this study were to monitor diurnal patterns of: (i) gaseous and dissolved metabolite concentrations in the bovine rumen, (ii) H2 and CH4 emitted, and (iii) the rumen microbiota. Furthermore, the effect of dietary inclusion of linseed oil on these patterns was assessed. Four rumen cannulated multiparous cows were used in a cross-over design with two 17 days periods and two dietary treatments: a control diet and a linseed oil supplemented diet [40% maize silage, 30% grass silage, 30% concentrate on dry matter (DM) basis for both diets; fat contents of 33 vs. 56 g/kg of DM]. On day 11, rumen contents were sampled for 10 h after morning feeding to profile gaseous and dissolved metabolite concentrations and microbiota composition. H2 and CH4 emission (mass per unit of time) was measured in respiration chambers from day 13 to 17. A 100-fold increase in ruminal H2 partial pressure (contribution to the total pressure of rumen headspace gases) was observed at 0.5 h after feeding. This peak was followed by a decline to basal level. Qualitatively similar patterns after feeding were also observed for H2 and CH4 emission, ethanol and lactate concentrations, and propionate molar proportion, although the opposite pattern was seen for acetate molar proportion. Associated with these patterns, a temporal biphasic change in the microbial composition was observed as based on 16S ribosomal RNA with certain taxa specifically associated with each phase. Bacterial concentrations (log10 16S ribosomal RNA gene copies based) were affected by time, and were increased by linseed oil supplementation. Archaeal concentrations (log10 16S ribosomal RNA gene copies based) tended to be affected by time and were not affected by diet, despite linseed oil supplementation decreasing CH4 emission, tending to decrease the partial pressure of CH4, and tending to increase propionate molar proportion. Linseed oil supplementation affected microbiota composition, and was most associated with an uncultivated Bacteroidales taxon. In summary, our findings support the importance of diurnal dynamics for the understanding of VFA, H2, and CH4 production.

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“…The main effect of LYC decreased (P ≤ 0.05) at h 2 and 2.5 and tended (P = 0.07) to decrease H 2 emission rate at h 3. The amount of H 2 emitted when methanogenesis is depressed appears to depend on the varying degree of H 2 (aq) supersaturation and greater release into the gas phase (Wang et al, 2016b). Yeast are thought to decrease the redox potential (a more negative E h ), either directly by scavenging O 2 or indirectly by stimulation of the general population of bacteria (Pinloche et al, 2013) .…”

Section: Methane and Hydrogen Productionmentioning

confidence: 99%

“…In contrast, Wang et al (2016a) argued that ruminal supersaturation could indeed influence thermodynam-ics calculations, which also was demonstrated in continuous culture (Wenner, 2016). Paradoxically, H 2 (aq) concentration was associated with greater numbers of methanogens and lower CH 4 emission (Wang et al, 2016b). Because many of the previously reported correlations ignored the fixed effects of diet, further research is needed to assess how H 2 (aq) can influence fermentation end products and community composition within and among diets.…”

Section: Introductionmentioning

confidence: 99%

Rumen microbial responses to supplemental nitrate. II. Potential interactions with live yeast culture on the prokaryotic community and methanogenesis in continuous culture

Welty

Wenner

Wagner

et al. 2019

Journal of Dairy Science

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Nitrates have been fed to ruminants, including dairy cows, as an electron sink to mitigate CH 4 emissions. In the NO 3 − reduction process, NO 2 − can accumulate, which could directly inhibit methanogens and possibly other microbes in the rumen. Saccharomyces cerevisiae yeast was hypothesized to decrease NO 2 − through direct reduction or indirectly by stimulating the bacterium Selenomonas ruminantium, which is among the ruminal bacteria most well characterized to reduce both NO 3 − and NO 2 −. Ruminal fluid was incubated in continuous cultures fed diets without or with NaNO 3 (1.5% of diet dry matter; i.e., 1.09% NO 3 −) and without or with live yeast culture (LYC) fed at a recommended 0.010 g/d (scaled from cattle to fermentor intakes) in a 2 × 2 factorial arrangement of treatments. Treatments with LYC had increased NDF digestibility and acetate: propionate by increasing acetate molar proportion but tended to decrease total VFA production. The main effect of NO 3 − increased acetate: propionate by increasing acetate molar proportion; NO 3 − also decreased molar proportions of isobutyrate and butyrate. Both NO 3 − and LYC shifted bacterial community composition (based on relative sequence abundance of 16S rRNA genes). An interaction occurred such that NO 3 − decreased valerate molar proportion only when no LYC was added. Nitrate decreased daily CH 4 emissions by 29%. However, treatment × time interactions were present for both CH 4 and H 2 emission from the headspace; CH 4 was decreased by the main effect of NO 3 − until 6 h postfeeding, but NO 3 − and LYC decreased H 2 emission up to 4 h postfeeding. As expected, NO 3 − decreased methane emissions in continuous cultures; however, contrary to expectations, LYC did not attenuate NO 2 − accumulation.

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Shifts in Rumen Fermentation and Microbiota Are Associated with Dissolved Ruminal Hydrogen Concentrations in Lactating Dairy Cows Fed Different Types of Carbohydrates

Cited by 69 publications

References 33 publications

Effect of changing forage on the dynamic variation in rumen fermentation in sheep

Effect of changing forage on the dynamic variation in rumen fermentation in sheep

Diurnal Dynamics of Gaseous and Dissolved Metabolites and Microbiota Composition in the Bovine Rumen

Rumen microbial responses to supplemental nitrate. II. Potential interactions with live yeast culture on the prokaryotic community and methanogenesis in continuous culture

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