Algal biofuel production requires CO 2 , electricity, and process heat. Previous studies assumed CO 2 sourcing from nearby coal or natural gas power plants. This may not be viable at a large scale or for the long term. The diurnal algal growth cycle imposes additional system design challenges for CO 2 delivery. For ethanol produced by cyanobacteria in photobioreactors, we design onsite systems that provide heat, power and CO 2 (CHP-CO 2), fueled by natural gas or biomass. Meeting the CO 2 requirement produces excess electricity, which can be sold back to the grid. The scale of the CHP-CO 2 can be reduced by nighttime capture and refrigerated storage of CO 2. The lifecycle greenhouse gas (GHG) emissions for 1 MJ ethanol are about −19 g CO 2 e for biomass CHP-CO 2 , and +31-35 CO 2 e g for natural gas CHP-CO 2 options, compared with +19 g CO 2 e for the direct use of coal flue gas, and 91.3 g CO 2 e for 1 MJ of conventional gasoline. This work evaluates the energy and GHG implications of onsite CHP-CO 2 for algal ethanol production and other CO 2 sourcing options. Combined heat and power (CHP) facilities, fueled by natural gas or biomass, could be co-located with algal ethanol production, capturing and utilizing carbon dioxide to make biofuel, and thus providing an essentially stand-alone biofuel operation, free from the constraints of co-location with anthropogenic sources.