Inkjet printed periodical micropatterns made of inert alumina ceramics induce contact guidance and stimulate osteogenic differentiation of mesenchymal stromal cells

Lauria, Ines; Krämer, M.; Schröder, Teresa; Kant, Sebastian; Hausmann, A.; Böke, Frederik; Leube, Rudolf E.; Telle, Rainer; Fischer, Horst

doi:10.1016/j.actbio.2016.08.004

Cited by 47 publications

(41 citation statements)

References 61 publications

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“…ALP activity in the supernatants of UC‐MSCs was significantly increased on Plasmapore surfaces when cultured under GM conditions without any osteogenic induction (Fig. ), which is in line with results on microstructured ceramic surfaces . Osteopontin gene expression was increased after 21 days, though the protein in the supernatant was reduced compared to the other surfaces.…”

Section: Discussionsupporting

confidence: 83%

“…Fluorescence microscopic imaging was performed as already described in Ref. . In brief, human UC‐MSCs were seeded at a density of 5000 cells/cm 2 and incubated in GM for 5 days, then washed once with prewarmed PBS and fixed with 4% (w/v) paraformaldehyde/PBS (Sigma‐Aldrich, Steinheim, Germany) for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of osteogenic gene expression by qPCR was performed as essentially described elsewhere . In brief, RNeasy Mini Kit and QIAshredder homogenizer (Qiagen, Hilden, Germany) were utilized to isolate total RNA, of which 0.5–0.8 µg served as a template for the cDNA synthesis by the First Strand cDNA Synthesis Kit (#K1612, Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

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Response of umbilical cord mesenchymal stromal cells to varying titanium topographical signals

Lauria

Höner

Kant

et al. 2017

J Biomedical Materials Res

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A wide variety of titanium implant modifications have been developed to improve tissue-or cell-material interactions including bone bonding, implant failure, and contact osteogenesis. Osteogenesis can be stimulated by mechanobiological signals such as topography though translation of in vivo reactions to in vitro bioactivity and stem cell culture data, and vice versa, is challenging. We hypothesized that a systematic in vitro approach comparing clinically wellaccepted implant surface topographical modifications could shed light on potential cell biological mechanisms provoked by submicron-, micron-or macrostructured surfaces. In this study, we investigated the response of umbilical cord derived mesenchymal stromal cells (UC-MSCs) to anodized, particle blasted, and plasma sprayed highly porous Plasmapore surfaces, which is known to promote bony ingrowth in vivo. After 21 days, UC-MSCs undergo a morphological shift from a 2D to 3D behavior on micro-or macrostructures visualized by actin-vinculin fluorescence and are able to fill the porous surfaces completely. Cell viability after 7 days was significantly decreased on the micro-and macrostructured surfaces particle blasted and Plasmapore, compared to polished controls. The analysis of osteogenic differentiation under noninduced conditions revealed a significantly elevated ALP activity on Plasmapore, indicating a beneficial effect of this macrostructured surface toward osteogenic differentiation supported by late elevated gene expression of osteopontin evaluated by qPCR. Mineralization as well as in vitro bioactivity was pronounced on anodized surfaces. Our findings point to synergistic implant modification strategies allowing early contact osteogenesis and bone ingrowth for future implant designs.

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Section: Discussionsupporting

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Response of umbilical cord mesenchymal stromal cells to varying titanium topographical signals

Lauria

Höner

Kant

et al. 2017

J Biomedical Materials Res

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“…During tissue formation and regeneration processes, cells respond to their environment by changing their initial adhesion, cell morphology, proliferation, migration behavior, and differentiation . Besides chemical signals, surface energy and elasticity, the cell response is regulated by the surface topography . Therefore, to tailor the outstanding properties of BGs and to stimulate cellular reactions such as cell differentiation, the two BGs 45S5 and 13–93 were cast creating microstructured groove‐and‐ridge surfaces of 30 µm ridge width, 10 µm groove width, and 8 or 15 µm groove depth, verified as amorphous by XRD (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…hMSCs were isolated from femoral heads after total hip arthroplasty after informed consent approved by the Ethics Committee of the Medical Faculty of RWTH Aachen University Hospital (EK 300/13) as described elsewhere . The hMSCs were then cultured in T75 culture flasks (VWR, Radnor) at 37°C, in 5% CO 2 and 90% humidity in hMSCs growth medium (GM; Mesenpan, PAN Biotech, Aidenbach, Germany) containing 2% FCS (Pan Biotech, Aidenbach, Germany) and 1% penicillin/streptomycin (Gibco, Invitrogen, Carlsbad, CA).…”

Section: Methodsmentioning

confidence: 99%

Periodic microstructures on bioactive glass surfaces enhance osteogenic differentiation of human mesenchymal stromal cells and promote osteoclastogenesis in vitro

Höner

Lauria

Dabhi

et al. 2018

J Biomedical Materials Res

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Bioactive glasses (BG) are known for their ability to bond to hard and soft tissues. We hypothesized that the stimulation of bone remodeling, including cellular bone forming and bone resorbing processes, can be increased by applying periodic microstructures on the glass surfaces in vitro. To test our hypothesis, two different BG (45S5 and 13-93) were microstructured in a groove-and-ridge pattern of different sizes by a novel casting process and tested in cell culture experiments using human mesenchymal stromal cells (hMSCs) and RAW 264.7 cells. The microstructures induced contact guidance of hMSCs and increased osteogenic marker gene expression of the stem cells, compared to non-structured glass surfaces as verified by ELISA and quantitative real-time PCR (qPCR) analyses. Furthermore, the structures stimulated the differentiation of RAW cells to osteoclast-like cells confirmed by TRAP gene expression and their resorption activity causing visible resorption lacunae. Our results demonstrate that periodically microstructured BG (especially 45S5) might improve the osteogenic differentiation of hMSCs and influence the activity of material resorbing cells in vitro. Hence, microstructuring of BG could enhance the remodeling process of bone substitutes critical for the formation of new bone tissue in vivo and thus be used to trigger bone remodeling kinetics in vivo. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1965-1978, 2018.

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Next Generation Cell Culture Tools Featuring Micro‐ and Nanotopographies for Biological Screening

Carthew

Abdelmaksoud

Cowley

et al. 2021

Adv Funct Materials

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Cells can perceive complex mechanical cues across both the micro‐ and nanoscale which can influence their development. While causative effects between surface topography and cellular function can be demonstrated, the variability in materials used in this screening process makes it difficult to discern whether the observed phenotypic changes are indeed a result of topographical cues alone or the inherent difference in material properties. A novel approach to directly imprint micro‐ and nanoscaled topographical features into the base of conventional cell cultureware is thus developed, facilitating its compatibility with standard biological techniques and methods of analysis. The utility of this technology is demonstrated by performing high‐throughput screening across five distinct cell types to interrogate the effects of 12 surface topographies, exemplifying unique cell‐specific responses to both behavior and cell morphological characteristics. The ability of this technology to underpin new insights into how surface topographies can regulate key image descriptors to drive cell fate determination is further demonstrated. These findings will inform the future development of advanced micro‐ and nanostructured cell culture substrates that can regulate cell behavior and fate determination across the life sciences, including fundamental cell biology, drug screening, and cell therapy.

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Inkjet printed periodical micropatterns made of inert alumina ceramics induce contact guidance and stimulate osteogenic differentiation of mesenchymal stromal cells

Cited by 47 publications

References 61 publications

Response of umbilical cord mesenchymal stromal cells to varying titanium topographical signals

Response of umbilical cord mesenchymal stromal cells to varying titanium topographical signals

Periodic microstructures on bioactive glass surfaces enhance osteogenic differentiation of human mesenchymal stromal cells and promote osteoclastogenesis in vitro

Next Generation Cell Culture Tools Featuring Micro‐ and Nanotopographies for Biological Screening

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