A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells

Abstract

BackgroundMesenchymal stromal cells (MSCs) are multipotent progenitor cells used in several cell therapies. MSCs are characterized by the expression of CD73, CD90, and CD105 cell markers, and the absence of CD34, CD45, CD11a, CD19, and HLA-DR cell markers. CD90 is a glycoprotein present in the MSC membranes and also in adult cells and cancer stem cells. The role of CD90 in MSCs remains unknown. Here, we sought to analyse the role that CD90 plays in the characteristic properties of in vitro expanded human MSCs.MethodsWe investigated the function of CD90 with regard to morphology, proliferation rate, suppression of T-cell proliferation, and osteogenic/adipogenic differentiation of MSCs by reducing the expression of this marker using CD90-target small hairpin RNA lentiviral vectors.ResultsThe present study shows that a reduction in CD90 expression enhances the osteogenic and adipogenic differentiation of MSCs in vitro and, unexpectedly, causes a decrease in CD44 and CD166 expression.ConclusionOur study suggests that CD90 controls the differentiation of MSCs by acting as an obstacle in the pathway of differentiation commitment. This may be overcome in the presence of the correct differentiation stimuli, supporting the idea that CD90 level manipulation may lead to more efficient differentiation rates in vitro.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-016-0359-3) contains supplementary material, which is available to authorized users.

Induced Pluripotent Stem Cell-Derived Mesenchymal Stromal Cells Are Functionally and Genetically Different From Bone Marrow-Derived Mesenchymal Stromal Cells

“…S3A). Although Moraes et al showed that depletion of CD90 in BM‐MSCs stimulated their osteogenic ability , this association was not found in iMSCs generated in our study (Fig. B).…”

Donor Variability in Growth Kinetics of Healthy hMSCs Using Manual Processing: Considerations for Manufacture of Cell Therapies

“…The ten batches processed displayed hMSC markers, although CD90 showed some variability relative to the other markers, suggesting that it may be a critical marker of product quality. Indeed, CD90 expression was demonstrated to regulate hMSC differentiation ability . However, this did not appear to impact on any of the donor hMSC's ability to undergo tri‐lineage differentiation.…”

Cannabidiol Modulates the Immunophenotype and Inhibits the Activation of the Inflammasome in Human Gingival Mesenchymal Stem Cells

“…Moreover, CBD treatment led to the down-regulation of the MSCs markers CD44, CD90, CD166. Interestingly, the downregulation of CD90, CD44, and CD166 has been linked to a more differentiated stem cell phenotype and to a less tumorigenic potential (Moraes et al, 2016). Additionally, we detected others surface markers involved the activation of the immune system which expression was downregulated by CBD such as CD109, CD151, CD40, CD46, CD59, CD68, CD81, CD82, CD99.…”