Detection of clarithromycin-resistant Helicobacter pylori in clinical specimens by molecular methods: A review

Xuan, Shi‐Ying; Wu, Lipei; Zhou, Yonggang; Xiao, Mingbing

doi:10.1016/j.jgar.2016.01.002

Cited by 29 publications

(26 citation statements)

References 75 publications

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“…Clarithromycin is a macrolide antibiotic that inhibits protein synthesis by targeting the 23S rRNA region in the 50S ribosomal subunit. Mutations A2143G and A2142G/C in the target region of the 23S rRNA are the most common causes of clarithromycin resistance in H. pylori in Europe (reviewed by Xuan et al [30]). The mechanism driving OMVmediated protection of H. pylori against clarithromycin in our study has not yet been elucidated.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of Helicobacter pylori membrane vesicles against stress and antimicrobial agents

et al. 2020

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Outer-membrane vesicles (OMVs) produced by Helicobacter pylori deliver bacterial components to host cells, provide a mechanism for stabilization of secreted components and may allow the bacteria to exert ‘long-range’ effects in the gastric niche, promoting persistence. In addition to their well-characterized host cell interactions, membrane vesicles improve stress survival in other bacterial species, and are constitutively produced by both pathogenic and non-pathogenic bacteria. We aimed to determine whether OMVs could improve H. pylori survival of a range of stressors. The effects of purified OMVs on the resistance of H. pylori to a range of environmental and antimicrobial stresses were determined using growth curves and survival assays. Addition of purified OMVs to H. pylori cultures provided dose-dependent protection against hydrogen peroxide-mediated killing. Supplementation with OMVs also partially protected H. pylori against the bactericidal effects of the antibiotics clarithromycin and levofloxacin, but not against amoxicillin nor metronidazole. Addition of purified OMVs allowed H. pylori to grow in the presence of inhibitory concentrations of the antimicrobial peptide LL-37. In the presence of 50 µg OMVs ml−1, significantly enhanced H. pylori growth was observed at higher LL-37 concentrations compared with lower LL-37 concentrations, suggesting that OMV–LL-37 interactions might facilitate release of growth-promoting nutrients. Taken together, these data indicate that production of membrane vesicles could help H. pylori to survive exposure to antibiotics and host antimicrobial defences during infection.

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Section: Discussionmentioning

confidence: 99%

Protective effects of Helicobacter pylori membrane vesicles against stress and antimicrobial agents

et al. 2020

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“…Until now PCR techniques and in particular real-time PCR are routine clinical methods for detection and identification of drug-resistant and susceptible strains of H. pylori; moreover, real-time PCR is easy to achieve, rapid, with high sensitivity (98%) and specificity (92%). It is also a cost effective technique [117][118][119][120][121][122][123][124].…”

Section: Invasive Diagnostic Testsmentioning

confidence: 99%

“…Using special parts of the body, the level of pH, the need of confocal laser endomicroscope, and high costs are important limitations for application of FIVH as a routine clinical method. However, there is promising future for FIVH by omitting the aforementioned limitations [121,125,126].…”

Section: Invasive Diagnostic Testsmentioning

confidence: 99%

Advances in diagnosis and treatment of Helicobacter pylori infection

Ranjbar

Behzadi

Farshad

2017

Acta Microbiologica et Immunologica Hungarica

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Helicobacter pylori is a Gram-negative motile bacterium causative agent of acute and chronic digestive and extra-digestive human infections. According to different reports worldwide, H. pylori symptomatic and asymptomatic infections are a global problem. The statistical investigations show a percentage of 50 for people who are involved in H. pylori acute/chronic digestive and/or extra-digestive infections around the world. This review focuses on digestive and extra-digestive diseases caused by H. pylori, the related virulence factors, diagnostic techniques including non-invasive and invasive diagnostics and treatment. There is an abundance of diagnostics for detection and identification of H. pylori. The availability, cost, and the condition of test performance may differ from place to place. To increase the level of reliability in association with diagnostic tools for detecting H. pylori, several techniques must be applied at once as multi-diagnostic technique. Furthermore, there are several pharmacotherapies which can be used for complete eradication of H. pylori infection.

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“…Mutations A2143G and A2142G/C are most often associated with clarithromycin resistance in Europe, Asia, South America, North America, and Africa, while A2144G was described in Asia and North America. Other 23S rRNA gene mutations, such as T2182C, C2611A, T2717C, and T2142C, are associated with low level resistance . A recent study in France reported that the main mutations were A2142/2143G (150/158; 94%) followed by A2142C (6/158 cases) and A2142T (2/158 cases) …”

Section: Introductionmentioning

confidence: 99%

“…Other 23S rRNA gene mutations, such as T2182C, C2611A, T2717C, and T2142C, are associated with low level resistance. 11,12 A recent study in France reported that the main mutations were A2142/2143G (150/158; 94%) followed by A2142C (6/158 cases) and A2142T…”

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confidence: 99%

Molecular diagnosis of Helicobacter pylori infection in gastric biopsies: Evaluation of the Amplidiag^®H. pylori + ClariR assay

et al. 2018

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Background Adapted treatments for Helicobacter pylori infection, guided by determining antimicrobial resistance, are associated with high eradication rates. We evaluated the performance of the Amplidiag® H. pylori + ClariR PCR assay (Amplidiag®) for detecting H. pylori and its clarithromycin resistance from gastric biopsies taken during endoscopy in comparison to culture and our “in‐house” PCR. Materials and Methods A total of 127 gastric biopsies were analyzed (98 adults; 29 children). Culture, PCR Amplidiag®, and in‐house PCR were performed in parallel. The in‐house PCR combined amplification and sequencing of a 267‐bp fragment of the H. pylori 23S rRNA gene. Discrepancies were controlled by amplification of glmM gene. Results For detection of H. pylori, Amplidiag® and the in‐house PCR were concordant in 118 of 127 of cases: 66 negative and 52 positive. Discrepancies were observed in nine cases, all with low bacterial load: Amplidiag® did not detect seven biopsies positive on in‐house PCR but detected two positive biopsies that were negative on in‐house PCR. Among the 19 of 52 (36%) H. pylori cases resistant to clarithromycin, only four biopsies with mixed populations exhibited discordant results between the two PCR methods. The A2142T mutation was not detected by Amplidiag®. With the in‐house PCR and amplified glmM gene as the reference method, the sensitivity and specificity of Amplidiag® was 88.5% (95% confidence interval 83‐94.1) and 100%. Conclusion This study demonstrated the high sensitivity of the PCR‐based Amplidiag® H. pylori test, especially with low H. pylori load, and the probability of its clarithromycin resistance analysis. For clinical use, a well‐designed trial with a large scale of samples may still be needed.

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Detection of clarithromycin-resistant Helicobacter pylori in clinical specimens by molecular methods: A review

Cited by 29 publications

References 75 publications

Protective effects of Helicobacter pylori membrane vesicles against stress and antimicrobial agents

Protective effects of Helicobacter pylori membrane vesicles against stress and antimicrobial agents

Advances in diagnosis and treatment of Helicobacter pylori infection

Molecular diagnosis of Helicobacter pylori infection in gastric biopsies: Evaluation of the Amplidiag^®H. pylori + ClariR assay

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Detection of clarithromycin-resistant Helicobacter pylori in clinical specimens by molecular methods: A review

Cited by 29 publications

References 75 publications

Protective effects of Helicobacter pylori membrane vesicles against stress and antimicrobial agents

Protective effects of Helicobacter pylori membrane vesicles against stress and antimicrobial agents

Advances in diagnosis and treatment of Helicobacter pylori infection

Molecular diagnosis of Helicobacter pylori infection in gastric biopsies: Evaluation of the Amplidiag®H. pylori + ClariR assay

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Molecular diagnosis of Helicobacter pylori infection in gastric biopsies: Evaluation of the Amplidiag^®H. pylori + ClariR assay