Labeling mesenchymal cells with DMSA-coated gold and iron oxide nanoparticles: assessment of biocompatibility and potential applications

Silva, Luisa H. A.; Silva, Jaqueline da; Ferreira, Guilherme A.; Silva, Renata C.; Lima, E.C.D.; Azevedo, Ricardo Bentes; Oliveira, Dutra de

doi:10.1186/s12951-016-0213-x

Cited by 41 publications

(31 citation statements)

References 56 publications

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“…In this context, one promising medical application of nanomaterials is the use of inorganic NPs within mesenchymal stromal cell (MSC)-based therapies. Nanomaterials have facilitated not only the acquisition of knowledge of stem cell biology but also allowed the development of new approaches that have enhanced their homing, survival, and biological effects (Silva et al 2016). Silva et al (2016) magnetized MSCs with iron oxide NPs, administered these cells through the jugular vein of silicotic mice, and exposed the animals to a localized magnetic field around the thorax.…”

Section: Silicosismentioning

confidence: 99%

“…Nanomaterials have facilitated not only the acquisition of knowledge of stem cell biology but also allowed the development of new approaches that have enhanced their homing, survival, and biological effects (Silva et al 2016). Silva et al (2016) magnetized MSCs with iron oxide NPs, administered these cells through the jugular vein of silicotic mice, and exposed the animals to a localized magnetic field around the thorax. The authors found that NPs were able to act as good agents for magnetic targeting of MSCs to the site of injury, resulting in increased cell retention in lung parenchyma and histologic improvement.…”

Section: Silicosismentioning

confidence: 99%

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New perspectives in nanotherapeutics for chronic respiratory diseases

et al. 2017

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According to the World Health Organization (WHO), hundreds of millions of people of all ages and in all countries suffer from chronic respiratory diseases, with particular negative consequences such as poor health-related quality of life, impaired work productivity, and limitations in the activities of daily living. Chronic obstructive pulmonary disease, asthma, occupational lung diseases (such as silicosis), cystic fibrosis, and pulmonary arterial hypertension are the most common of these diseases, and none of them are curable with current therapies. The advent of nanotechnology holds great therapeutic promise for respiratory conditions, because non-viral vectors are able to overcome the mucus and lung remodeling barriers, increasing pharmacologic and therapeutic potency. It has been demonstrated that the extent of pulmonary nanoparticle uptake depends not only on the physical and chemical features of nanoparticles themselves, but also on the health status of the organism; thus, the huge diversity in nanotechnology could revolutionize medicine, but safety assessment is a challenging task. Within this context, the present review discusses some of the major new perspectives in nanotherapeutics for lung disease and highlights some of the most recent studies in the field.

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Section: Silicosismentioning

confidence: 99%

Section: Silicosismentioning

confidence: 99%

New perspectives in nanotherapeutics for chronic respiratory diseases

et al. 2017

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“…15,16 Iron oxide nanoparticles have been widely reported to produce highly reactive hydroxyl radicals. 17 These radicals and the subsequent oxidative stress are considered biotoxic and have the ability to inhibit chondrogenic differentiation. [18][19][20][21] In this study, the concentration of ferucarbotran necessary to inhibit the chondrogenic differentiation of MSCs varied across donors, probably due to the variation in the amount of intracellularly incorporated iron among the donors.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Safety and Quality of Magnetically Labeled Human Mesenchymal Stem Cells Preparation for Cartilage Repair

Negi

Takeuchi²,

Kamei

et al. 2019

Tissue Engineering Part C: Methods

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Magnetic delivery system of mesenchymal stem cells (MSCs) has been developed for cartilage repair. It provides an effective and minimally invasive method for MSC transplantation. In this study, we evaluated the safety and quality of magnetically labeled human MSCs for practical applications. The safety of magnetically labeled MSCs was assessed using karyotyping, colony-forming assay using soft agar, and cell proliferation in a long-term culture. Magnetic labeling did not affect the karyotype, and MSCs retained their ability to grow and proliferate in an anchorage-independent manner even after exposure to magnetic force. The quality of the magnetically labeled MSCs was assessed by chondrocyte differentiation and reactivity toward magnetic forces. Magnetic labeling inhibited the chondrogenic differentiation of MSCs at higher densities of magnetic particles. MSCs labeled with ferucarbotran nanoparticles were retained by magnetic forces in a dose-dependent manner. The magnetization of MSCs with an appropriate density of magnetic particles maintained both qualities in MSCs. However, the uptake quantity of iron into MSCs varied across donors, even for the same density of magnetic particles. Therefore, the appropriate density of magnetic particles for use in MSC delivery for cartilage repair should be examined for every donor before treatment.

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“…Other coatings, namely DMSA [37,38], poly(L-lactic acid) [39], citrate [40], oleic acid [41], or oleate [42], and their derivatives, were only used in a few studies, and their biological effects and properties are poorly studied.…”

Section: Coatingmentioning

confidence: 99%

Physicochemical Properties of Magnetic Nanoparticles: Implications for Biomedical Applications In Vitro and In Vivo

Belanova¹,

Gavalas

Makarenko

et al. 2018

Oncol Res Treat

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Magnetic and superparamagnetic iron oxide nanoparticles are emerging as promising candidates for various applications in biology and medicine, and especially in oncology. These applications, however, require that a specific set of physical, chemical, and biological properties be combined in a given sample of nanoparticles for them to act as intended. Some of these properties are fundamental: They strictly determine the nanoparticles' behavior both in vitro and in vivo. These properties are the charge, the solution stability and zeta potential, and the coating of the nanoparticles. A certain combination of these properties may satisfy a researcher in an in vitro study, but other properties should also be considered when in vivo applications are planned. For in vivo experiments, additional determinants of the quality of nanoparticles are their size, shape, modifications with targeting moieties, and degradation/excretion pathways. All these properties are in the focus of the present review.

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Labeling mesenchymal cells with DMSA-coated gold and iron oxide nanoparticles: assessment of biocompatibility and potential applications

Cited by 41 publications

References 56 publications

New perspectives in nanotherapeutics for chronic respiratory diseases

New perspectives in nanotherapeutics for chronic respiratory diseases

In Vitro Safety and Quality of Magnetically Labeled Human Mesenchymal Stem Cells Preparation for Cartilage Repair

Physicochemical Properties of Magnetic Nanoparticles: Implications for Biomedical Applications In Vitro and In Vivo

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