Molecular cloning and expression vector construction of bovine TRIM28

Abstract

ABSTRACT. The bovine TRIM28 gene was amplified from ovary tissue by using RT-PCR. The TRIM28 gene was inserted into the eukaryotic expression vector pIRES2-EGFP and transfected into bovine fetal fibroblasts by using Lipofectamine 3000. TRIM28 mRNA and protein were detected by fluorescence microscope and western blotting. The results showed that the full length of TRIM28 was cloned and pIRES2-EGFP-TRIM28 was constructed successfully. EGFP expression was observed, and the pIRES2-EGFP-TRIM28 transfected group expressed more TRIM28 protein than that by the pIRES2-EGFP group. The TIMR28 gene has been successfully transferred into bovine fetal fibroblasts.

Effect of TRIM28 on proliferation, apoptosis and histone H3K9 trimethylation in bovine fibroblasts

“…Plasmid DNA was obtained from Escherichia coli. In our study, the restructured p EGFP-IRES2-TRIM28 plasmid was prepared by a previous experiment (Ma et al, 2016).…”