Optimization of desolvation process for fabrication of lactoferrin nanoparticles using quality by design approach

Pandey, Abhijeet; More, Mahesh P.; Karande, Kiran P; Chitalkar, Ramesh V; Patil, Pravin O.; Deshmukh, Prashant K.

doi:10.1080/21691401.2016.1202259

Cited by 16 publications

(9 citation statements)

References 37 publications

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“…While we initially chose ethanol for its limited toxicity, others have found that desolvation with methanol and ethanol blends can produce nanoclusters in the sub 100-nm range, while blends of ethanol and acetone increase the particle size into the 140-to 300-nm range (44). Isopropanol is another solvent commonly used for desolvation (46). We have also performed experiments using different desolvation reagents (ethanol, methanol, or isopropanol) at the same desolvation ratio (solvent:solution 4:1) to desolvate NP peptide, recombinant M2e-NA tetramer protein, or OVA into nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

Heterosubtypic influenza protection elicited by double-layered polypeptide nanoparticles in mice

Deng

Chang

Wang

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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Influenza is a persistent threat to public health. Here we report that double-layered peptide nanoparticles induced robust specific immunity and protected mice against heterosubtypic influenza A virus challenges. We fabricated the nanoparticles by desolvating a composite peptide of tandem copies of nucleoprotein epitopes into nanoparticles as cores and cross-linking another composite peptide of four tandem copies of influenza matrix protein 2 ectodomain epitopes to the core surfaces as a coating. Delivering the nanoparticles via dissolvable microneedle patch-based skin vaccination further enhanced the induced immunity. These peptide-only, layered nanoparticles demonstrated a strong antigen depot effect and migrated into spleens and draining (inguinal) lymph nodes for an extended period compared with soluble antigens. This increased antigen-presentation time correlated with the stronger immune responses in the nanoparticle-immunized group. The protection conferred by nanoparticle immunization was transferable by passive immune serum transfusion and depended partially on a functional IgG receptor FcγRIV. Using a conditional cell depletion, we found that CD8 T cells were involved in the protection. The immunological potency and stability of the layered peptide nanoparticles indicate applications for other peptide-based vaccines and peptide drug delivery.

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Section: Discussionmentioning

confidence: 99%

Heterosubtypic influenza protection elicited by double-layered polypeptide nanoparticles in mice

Deng

Chang

Wang

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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“…The percentage yield of formulated nanoformulations was determined by taking the dry weight of the nanoformulation 21 and using the following formula:…”

Section: Percentage Yield Determinationmentioning

confidence: 99%

A Systematic Approach to Vildagliptin-Eudragit RL 100 Coated Nanoparticles by Combined Techniques of Solvent Evaporation and Nano-precipitation Using Quality by Design Concept

Ammanage,

Mastiholimath

2023

Int J. Pharm. Investigation

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Background: Diabetes is an endocrine disorder characterized by an abnormal increase in blood sugar level. Vildagliptin is a Dipeptidyl Peptidase-4 (DPP-4) inhibitor used in the treatment of type 2 diabetes mellitus. Purpose: The present work deals with the Quality by Design (QbD) approach based formulation and optimization of Vildagliptin-Eudragit RL 100 coated nanoparticles (VLD-EudNPs). Materials and Methods: VLD-Eud NPs were developed by combined technique of solvent evaporation and nano-precipitation. Optimization was done by 3 2 factorial designs. Prepared nanoformulations were characterized for percentage yield and morphology. Further in vitro drug release and kinetics were studied. The accelerated stability study of the optimized formulation was carried out for 3 months. Results: VLD-EudNPs ranged from 58.5±2.02 nm to 425.4±4.71 nm with a PDI of 0.27±0.036 to 0.52±0.086. Entrapment efficiency (% EE) of nanoformulations F1 to F9 ranged between 32.29±1.16 to 75.26±1.63%. The particles were found to be cylindrical-shaped, with a percentage yield of 54.7±3.45 to 84.4±1.51%. During the in vitro drug release study, at acidic pH, <5% drug release, while delayed release at basic pH, at the end of 24 hr, all formulations have released >68% of the drug following zero order kinetics. The optimized formulation has proven its stability during stability study. Conclusion: The present work concludes that prepared and optimized VLD-Eud NPs could be a better approach for sustained delivery of Vildagliptin for the management of diabetes mellitus as compared to traditional drug delivery.

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“… [ 124 ] Lf NPs Desolvation CUR In vitro optimization The NPs exhibited a particle size of 214.7 nm with encapsulation efficiency of 53.7%. [ 99 ] Mann-Lf NPs Thermal treatment Shikonin JQ1 Colorectal cancer The NPs elicited immunogenic cell death and repolarizing TAMs to M1 macrophages. [ 102 ] TPGS-TAT Lf NPs Thermal treatment Simvastatin Fenretinide Glioma The NPs repolarized TAMs to M1 phenotype & increased ROS-induced mitochondrial apoptosis.…”

Section: Preparation Techniques Of Lactoferrin Nanocarriersmentioning

confidence: 99%

“…glutaraldehyde solution was added to harden the particles. Curcumin-loaded Lf NPs prepared by desolvation exhibited a particle size of 214.7 nm with encapsulation efficiency of 53.7% [ 99 ]. The nanoparticles were optimized by varying different factors e.g.…”

Section: Preparation Techniques Of Lactoferrin Nanocarriersmentioning

confidence: 99%

Lactoferrin, a multi-functional glycoprotein: Active therapeutic, drug nanocarrier & targeting ligand

et al. 2020

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Recent progress in protein-based nanomedicine, inspired by the success of Abraxane® albumin-paclitaxel nanoparticles, have resulted in novel therapeutics used for treatment of challenging diseases like cancer and viral infections. However, absence of specific drug targeting, poor pharmacokinetics, premature drug release, and off-target toxicity are still formidable challenges in the clinic. Therefore, alternative protein-based nanomedicines were developed to overcome those challenges. In this regard, lactoferrin (Lf), a glycoprotein of transferrin family, offers a promising biodegradable well tolerated material that could be exploited both as an active therapeutic and drug nanocarrier. This review highlights the major pharmacological actions of Lf including anti-cancer, antiviral, and immunomodulatory actions. Delivery technologies of Lf to improve its pries and enhance its efficacy were also reviewed. Moreover, different nano-engineering strategies used for fabrication of drug-loaded Lf nanocarriers were discussed. In addition, the use of Lf for functionalization of drug nanocarriers with emphasis on tumor-targeted drug delivery was illustrated. Besides its wide application in oncology nano-therapeutics, we discussed the recent advances of Lf-based nanocarriers as efficient platforms for delivery of anti-parkinsonian, anti-Alzheimer, anti-viral drugs, immunomodulatory and bone engineering applications.

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Optimization of desolvation process for fabrication of lactoferrin nanoparticles using quality by design approach

Cited by 16 publications

References 37 publications

Heterosubtypic influenza protection elicited by double-layered polypeptide nanoparticles in mice

Heterosubtypic influenza protection elicited by double-layered polypeptide nanoparticles in mice

A Systematic Approach to Vildagliptin-Eudragit RL 100 Coated Nanoparticles by Combined Techniques of Solvent Evaporation and Nano-precipitation Using Quality by Design Concept

Lactoferrin, a multi-functional glycoprotein: Active therapeutic, drug nanocarrier & targeting ligand

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