Mapping of Novel QTL Regulating Grain Shattering Using Doubled Haploid Population in Rice (<i>Oryza sativa</i>L.)

Lee, Gyu-Ho; Kang, In‐Kyu; Kim, Kyung Min

doi:10.1155/2016/2128010

Cited by 11 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The SHAT1 (Lin et al, 2007; Zhou et al, 2012), OsCPL1 (Ji et al, 2006, 2010) and OsSh1 (Lin et al, 2012) were mapped to chromosome 4, 7, and 3, respectively. Recently, two regions of the QTL for seed shattering on chromosome 1 and chromosome 6 were detected in rice (Lee et al, 2016). By comparison, the positions of two QTL in previous studies were similar to the QTL detected in this study.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Quantitative Trait Loci for Shattering in Japonica Rice Landrace Jiucaiqing from Taihu Lake Valley, China

Jiaan

Zhan

et al. 2016

The Plant Genome

View full text Add to dashboard Cite

Easy shattering reduces yield from grain loss during rice (Oryza sativa L.) harvest. We characterized a nonshattering japonica rice landrace Jiucaiqing from Taihu Lake valley in China. The breaking tensile strength (BTS; grams force, gf) of the grain pedicel was measured using a digital force gauge to evaluate the degree of shattering at 0, 7, 14, 21, 28, and 35 d after heading (DAH). The BTS of Jiucaiqing did not significantly decrease with increasing DAH, maintaining a level of 152.2 to 195.9 gf, while that of indica IR26 decreased greatly during 0 to 14 DAH and finally stabilized at ~100 gf. Then the chromosome segment substitution lines (CSSLs) and near isogenic lines (NILs) of Jiucaiqing in IR26 background were developed for quantitative trait loci (QTL) mapping. Four putative QTL (qSH1 JCQ , qSH3 JCQ , qSH6 JCQ , and qSH11 JCQ ) for shattering were detected, and the qSH1 JCQ was confirmed on chromosome 1. We further mapped qSH1 JCQ to a 98.4-kb region, which contains 14 genes. Os01g62920 was considered to be a strong candidate for qSH1 JCQ , which colocated with qSH1. Further quantitative realtime polymerase chain reaction (PCR) analyses confirmed that the QTL qSH1 JCQ can significantly decrease the expression of shattering related genes (qSH1, OsCPL1, Sh4, SH5, and SHAT1) especially at the middle development stage at 10 and 15 cm panicle length, which causes rice shattering decrease. The elite allele and the NIL with desirable agronomic traits identified in this study could be useful for rice breeding.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Quantitative Trait Loci for Shattering in Japonica Rice Landrace Jiucaiqing from Taihu Lake Valley, China

Jiaan

Zhan

et al. 2016

The Plant Genome

View full text Add to dashboard Cite

show abstract

“…The degree of seed shattering varies greatly among different rice accessions. Wild rice ( O. rufipogon and O. nivara) and weedy rice shed seeds very easily while the majority of cultivated rice show no or limited shattering 30 . Within cultivated rice, generally indica cultivars shatter seed easily than japonica cultivars 26 .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide discovery of DNA polymorphisms by whole genome sequencing differentiates weedy and cultivated rice

Chai

Shankar

Jain

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Analyzing the genome level DNA polymorphisms between weedy and cultivated rice is crucial to elucidate the molecular basis of weedy and agronomic traits, which in turn can enhance our ability to control weedy rice and its utilization for rice improvement. Here, we presented the genome-wide genetic variations between a weedy rice accession PSRR-1 and two cultivated rice accessions, Bengal and Nona Bokra, belonging to japonica and indica subspecies, respectively. The total number of SNPs and InDels in PSRR/Bengal was similar to that of Nona Bokra/Bengal, but was three times greater than that of PSRR/Nona Bokra. There were 11546 large-effect SNPs/InDels affecting 5673 genes, which most likely differentiated weedy rice from cultivated rice. These large effect DNA polymorphisms were mostly resulted in stop codon gain and least by start codon loss. Analysis of the molecular functions and biological processes of weedy rice specific SNPs/InDels indicated that most of these genes were involved in protein modification/phosphorylation, protein kinase activity, and protein/nucleotide binding. By integrating previous QTL mapping results with the DNA polymorphisms data, the candidate genes for seed dormancy and seed shattering were narrowed down. The genomic resource generated in this study will facilitate discovery of functional variants for weedy and agronomic traits.

show abstract

“…In rice breeding, the development of new varieties is one of the main goals, and DH technology plays a vital role in achieving this. In particular, DH technology is considered helpful for generating inbred lines from rice hybrids in a single generation and mapping quantitative trait loci (QTL) [350][351][352]. QTL analysis in DH progenies allowed the identification of genes involved in resistance to biotic (e.g., Cnaphalocrocis medinalis, Guenée [353], Magnaporthe grisea [354], and Xanthomonas oryzae pv.…”

Section: Monocot Speciesmentioning

confidence: 99%

Embryo Rescue in Plant Breeding

Rogo,

Fambrini,

Pugliesi

2023

Plants

View full text Add to dashboard Cite

Embryo rescue (ER) techniques are among the oldest and most successful in vitro tissue culture protocols used with plant species. ER refers to a series of methods that promote the development of an immature or lethal embryo into a viable plant. Intraspecific, interspecific, or intergeneric crosses allow the introgression of important alleles of agricultural interest from wild species, such as resistance or tolerance to abiotic and biotic stresses or morphological traits in crops. However, pre-zygotic and post-zygotic reproductive barriers often present challenges in achieving successful hybridization. Pre-zygotic barriers manifest as incompatibility reactions that hinder pollen germination, pollen tube growth, or penetration into the ovule occurring in various tissues, such as the stigma, style, or ovary. To overcome these barriers, several strategies are employed, including cut-style or graft-on-style techniques, the utilization of mixed pollen from distinct species, placenta pollination, and in vitro ovule pollination. On the other hand, post-zygotic barriers act at different tissues and stages ranging from early embryo development to the subsequent growth and reproduction of the offspring. Many crosses among different genera result in embryo abortion due to the failure of endosperm development. In such cases, ER techniques are needed to rescue these hybrids. ER holds great promise for not only facilitating successful crosses but also for obtaining haploids, doubled haploids, and manipulating the ploidy levels for chromosome engineering by monosomic and disomic addition as well substitution lines. Furthermore, ER can be used to shorten the reproductive cycle and for the propagation of rare plants. Additionally, it has been repeatedly used to study the stages of embryonic development, especially in embryo-lethal mutants. The most widely used ER procedure is the culture of immature embryos taken and placed directly on culture media. In certain cases, the in vitro culture of ovule, ovaries or placentas enables the successful development of young embryos from the zygote stage to maturity.

show abstract

Mapping of Novel QTL Regulating Grain Shattering Using Doubled Haploid Population in Rice (Oryza sativaL.)

Cited by 11 publications

References 28 publications

Identification and Characterization of Quantitative Trait Loci for Shattering in Japonica Rice Landrace Jiucaiqing from Taihu Lake Valley, China

Identification and Characterization of Quantitative Trait Loci for Shattering in Japonica Rice Landrace Jiucaiqing from Taihu Lake Valley, China

Genome-wide discovery of DNA polymorphisms by whole genome sequencing differentiates weedy and cultivated rice

Embryo Rescue in Plant Breeding

Contact Info

Product

Resources

About