Species-Specific Detection of Mycosphaerella polygoni-cuspidati as a Biological Control Agent for Fallopia japonica by PCR Assay

Kurose, Daisuke; Furuya, Naruto; Saeki, Takao; Tsuchiya, Kenichi; Tsushima, Seiya; Seier, M. K.

doi:10.1007/s12033-016-9962-x

Cited by 8 publications

(6 citation statements)

References 25 publications

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“…Some species of most abundant microbial genera we found (e.g. Pseudomonas and Mycosphaerella) have been previously reported for acting as biocontrol agents or BCAs (Kurose et al 2016;Jousset et al 2006). An interesting question would be to evaluate how to integrate microbial community studies into traditional biocontrol approaches?…”

Section: Discussionsupporting

confidence: 55%

Assessing the impact of plant genetic diversity in shaping the microbial community structure of Vitis vinifera phyllosphere in the Mediterranean

Singh

Gobbi

Santoni

et al. 2018

Frontiers in Life Science

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The aerial surface of the plant (phyllosphere) is the habitat of complex microbial communities and the structure of this microbiome may be dependent on plant genetic factors, local environment or interactions between them. In this study, we explored the microbial diversity present in the phyllosphere of a very diverse set of grapevine cultivars representing the three genetic pools of the species, grown on an experimental plot at Montpellier (French Mediterranean region). We assessed microbiome variation in the phyllosphere using amplicon sequencing of the 16S rRNA gene and of the internal transcribed spacer (ITS), according to the grapevine genetic pools or cultivars, and organs (i.e. leaves and grape berries). The observed microbiome was complex; out of 542 bacterial genera; Pseudomonas, Pantoea, Sphingomonas, and Acinetobacter were the most abundant and almost ubiquitously present across the samples, and out of 267 fungal genera; Aureobasidium, Alternaria, Mycosphaerella and Aspergillus were most represented. Our results illustrated that the microbial taxa were almost uniformly distributed among the genetic pools and only a few cultivar or genetic pool level differences were found, but a very clear differential taxa abundance was found between the leaf and berry samples. Some genus level associations were also observed with certain genetic pools.

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Section: Discussionsupporting

confidence: 55%

Assessing the impact of plant genetic diversity in shaping the microbial community structure of Vitis vinifera phyllosphere in the Mediterranean

Singh

Gobbi

Santoni

et al. 2018

Frontiers in Life Science

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“…Collapsimycopappus is closely related to three Mycodiella species and “ Mycosphaerella ” polygoni ‐ cuspidati (100% ML BP/1.00 Bayesian PP, Figure ), but these four species are known to have only sexual morphs in common (Crous, Verkley, & Groenewald, ; Crous, Wingfield, Burgess et al., ; Crous, Wingfield, Richardson et al., ; Ito, Sato, & Ota, ; Kurose et al., , ). Collapsimycopappus is similar to Polythrincium in having sporodochial conidiomata and melanized conidia (Simon, Groenewald, & Crous, ), but differs in its lack of sympodially growing conidiophores with a spiral appearance.…”

Section: Resultsmentioning

confidence: 99%

“…“ Mycosphaerella ” polygoni ‐ cuspidati is known to cause leaf spot disease in Fallopia japonica (Houtt.) Ronse Decr., and its life cycle involving a sexual morph and spermatial stage has been described (Kurose et al., , ). “ Mycosphaerella ” polygoni‐cuspidati is related to Mycod .…”

Section: Discussionmentioning

confidence: 99%

“…"Mycosphaerella" polygoni-cuspidati is known to cause leaf spot disease in Fallopia japonica (Houtt.) Ronse Decr., and its life cycle involving a sexual morph and spermatial stage has been described (Kurose et al, 2009(Kurose et al, , 2016 Funk & Dorworth, 1987;Gross et al, 2017;Hashimoto et al, 2017;Wei et al, 1997Wei et al, , 1998 and Sclerotiniaceae (Helotiales, Leotiomycetes;Redhead & White, 1985;Suto & Kawai, 2000;Suto & Suyama, 2005;Takahashi et al, 2006). This appears to have resulted from convergent evolution of a similar ecological function, such as rain-splash dispersal across the leaf surface.…”

Section: Discussionmentioning

confidence: 99%

“…The pathogens, which were reisolated from the necrotic areas (Table 1), were morphologically and phylogenetically identical to the inoculated fungus. Collapsimycopappus is closely related to three Mycodiella species and "Mycosphaerella" polygoni-cuspidati (100% ML BP/1.00 Bayesian PP, Figure 1), but these four species are known to have only sexual morphs in common (Crous, Verkley, & Groenewald, 2006;Crous, Wingfield, Burgess et al, 2016;Crous, Wingfield, Richardson et al, 2016;Ito, Sato, & Ota, 1957;Kurose et al, 2009Kurose et al, , 2016.…”

Section: Inoculation Experimentsmentioning

confidence: 99%

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Collapsimycopappus: A new leaf pathogen with mycopappus‐like propagules in Mycosphaerellaceae

et al. 2018

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A previously undescribed mycopappus‐like fungus associated with large zonate leaf spot disease of Styrax obassia was found in Japan. Single‐propagule isolation of the fungus from necrotic areas of the diseased leaves yielded four pure cultures. Pathogenicity tests of the fungus were performed on potted S. obassia plants. Leaves were wounded with a sterilized needle and inoculated with mycelial agar. Development of necrotic zones was observed within 4 months. Numerous propagules appeared on the necrotic areas, and two strains of the same fungus were isolated again. The morphology of the pathogen was described, and its phylogeny was analysed based on the sequences of nuclear rDNA ITS, LSU regions and the rpb2 genes. Twenty‐four sequences were generated from six strains. Phylogenetic analyses indicated that the pathogen was closely related to the members of Mycosphaerellaceae, but was distinct in having brown propagules with hyphal appendages that collapsed at maturity and divided into part spores. Therefore, we propose Collapsimycopappus as a new genus to accommodate a single species, C. styracis.

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Exploiting exotic pathogens as mycoherbicides against invasive alien weeds: Japanese knotweed as a case study

Kurose

Seier

Evans

2023

Pest Management Science

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Plant species have been introduced increasingly into non‐native ranges, where many have become exotic weeds with adverse impacts on native ecosystems, as well as on farming and other livelihoods. In biological control, the classical or inoculative approach is the one most commonly used for the management of invasive alien weeds and is based on the use of co‐evolved natural enemies from the native range to control the invasive weed. Typically, the inundative or mycoherbicide approach targets problematic weeds using local plant pathogens that, in the case of introduced species, have ‘jumped’ onto the exotic host. The leaf‐spot fungus, Mycosphaerella polygoni‐cuspidati, co‐evolved with its host, Reynoutria (Fallopia) japonica (Japanese knotweed), in Japan and has a unique history of being investigated both as a classical biological control agent and a mycoherbicide against this highly invasive weed in the United Kingdom and North America. Here, we highlight our research on M. polygoni‐cuspidati as part of a biological control programme for Japanese knotweed and review the potential of mycoherbicides using exotic pathogens for the management of invasive alien weeds. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Species-Specific Detection of Mycosphaerella polygoni-cuspidati as a Biological Control Agent for Fallopia japonica by PCR Assay

Cited by 8 publications

References 25 publications

Assessing the impact of plant genetic diversity in shaping the microbial community structure of Vitis vinifera phyllosphere in the Mediterranean

Assessing the impact of plant genetic diversity in shaping the microbial community structure of Vitis vinifera phyllosphere in the Mediterranean

Collapsimycopappus: A new leaf pathogen with mycopappus‐like propagules in Mycosphaerellaceae

Exploiting exotic pathogens as mycoherbicides against invasive alien weeds: Japanese knotweed as a case study

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