The impact of environmental conditions on <i>Campylobacter jejuni</i> survival in broiler faeces and litter

Smith, Shaun; Meade, Joseph; Gibbons, J. F.; McGill, Kevina; Bolton, Declan; Whyte, Paul

doi:10.3402/iee.v6.31685

Cited by 23 publications

(27 citation statements)

References 44 publications

(52 reference statements)

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“…Standard crate washing procedures are largely ineffective in removing pathogens such as Campylobacter (Slader et al ), partly because of the difficulties in cleaning a complex plastic surface. It has been shown that Campylobacter jejuni can survive at least 48 h in broiler faeces (Smith et al ). Genotypes of Campylobacter detected in transport crates can be found in residual flocks after thinning, and also in birds sampled at the abattoir (Agunos et al ).…”

Section: Introductionmentioning

confidence: 99%

An improved cleaning system to reduce microbial contamination of poultry transport crates in the United Kingdom

Atterbury

Gigante

Tinker³

et al. 2020

J Appl Microbiol

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Aim Following previous research on improving the cleaning of crates used to transport broiler chickens from the farm to the abattoir, a demonstration project was undertaken to investigate improvements in crate washing on a commercial scale. Methods and Results The soak tank of a conventional crate washing system was replaced with a high‐performance washer fitted with high‐volume, high‐pressure nozzles. The wash water could be heated, and a greatly improved filtration system ensured that the nozzles did not lose performance or become blocked. Visual cleanliness scores and microbial counts were determined for naturally contaminated crates which had been randomly assigned to different cleaning protocols. Conclusions When a combination of mechanical energy, heat and chemicals (i.e. detergent and disinfectant) was used, the results showed significant improvements to crate cleaning. Reductions of up to 3·6 and 3·8 log10 CFU per crate base were achieved for Campylobacter and Enterobacteriaceae, respectively, along with a marked improvement in visual cleanliness. Significance and Impact of the Study Broiler transport crates may become heavily contaminated with faeces and this may contribute to the spread of disease between farms. The results of this trial may be of use in reducing the spread of zoonotic pathogens in the poultry meat supply chain.

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Section: Introductionmentioning

confidence: 99%

An improved cleaning system to reduce microbial contamination of poultry transport crates in the United Kingdom

Atterbury

Gigante

Tinker³

et al. 2020

J Appl Microbiol

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“…Note that members of Campylobacteraceae are livestock and poultry associated (Mughini Gras et al 2012), both of which are farmed in the study area. This Campylobacteraceae prevalence, concentration and survival during winter compared to other seasons in AGR, may be associated to with their sensitivity to desiccation during drier months (Smith et al 2016), occurrence of heavy rainfall (Ahmed et al 2013; Moriarty et al 2011), their presence in waterfowl feces (Moriarty et al 2012), or various farming practices (Rapp et al 2014). Alternatively this could also represent detection of material from bacterial lysis (Feng et al 2017).…”

Section: Resultsmentioning

confidence: 99%

Microbial community dynamics of surface water in British Columbia, Canada

Uyaguari

Croxen

Cronin

et al. 2019

Preprint

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Traditional methods for monitoring the microbiological quality of water focus on the detection of fecal indicator bacteria such as Escherichia coli, often tested as a weekly grab sample. To understand the stability of E.coli concentrations over time, we evaluated three approaches to measuring E. coli levels in water: microbial culture using Colilert, quantitative PCR for uidA and next-generation sequencing of the 16S rRNA gene. Two watersheds, one impacted by agricultural and the other by urban activities, were repeatedly sampled over a simultaneous ten-hour period during each of the four seasons. Based on 16S rRNA gene deep sequencing, each watershed showed different microbial community profiles. The bacterial microbiomes varied with season, but less so within each 10-hour sampling period. Enterobacteriaceae comprised only a small fraction (<1%) of the total community. The qPCR assay detected significantly higher quantities of E. coli compared to the Colilert assay and there was also variability in the Colilert measurements compared to Health Canada’s recommendations for recreational water quality. From the 16S data, other bacteria such as Prevotella and Bacteroides showed promise as alternative indicators of fecal contamination. A better understanding of temporal changes in watershed microbiomes will be important in assessing the utility of current biomarkers of fecal contamination, determining the best timing for sample collection, as well as searching for additional microbial indicators of the health of a watershed.

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“…24 There is limited knowledge about how Campylobacter persists in broiler litter and faeces. 25 C. jejuni survives significantly longer in faeces, with a minimum survival time of 48 hours, compared with 4 hours in used broiler litter. C. jejuni survival is significantly enhanced at 20˚C in all environmental conditions in both broiler litter and faeces, compared with survival at 25˚C and 30˚C.…”

mentioning

confidence: 86%

“…The persistence of Campylobacter in broiler litter and faeces under various environmental conditions has implications for farm litter management, hygiene, and disinfection practices. 25 The colonisation of broilers with Campylobacter in drinking water may be partly due to Campylobacter resisting disinfection inside waterborne protozoa. 26 Campylobacter jejuni inside amoeba can infect broilers.…”

mentioning

confidence: 99%