Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene

Abstract

The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine

“…Human embryonic stem cells (hESC) are stem cells derived directly from the inner cell mass of preimplantation embryo, which in humans comprises less than a dozen cells found at day 5–6 of development. HESCs are widely used in medical research and clinical grade hESCs, suitable for therapeutic applications, are readily available. − Induced pluripotent stem cells (iPSCs) have similar properties of continuous replication without differentiation (i.e., self-renewal) and have shown the ability to generate virtually any cell type in the body (i.e., they are pluripotent) if given the correct induction cues. They can be generated by genetic reprogramming of somatic cells, i.e., nonreproductive body cells .…”