A rapid and sensitive assay based on particle analysis for cell degranulation detection in basophils and mast cells

Huang, Lufen; Pi, Jiang; Wu, Jian‐Lin; Zhou, Hua; Cai, Jiye; Li, Ting; Liu, Liang

doi:10.1016/j.phrs.2016.05.033

Cited by 31 publications

(19 citation statements)

References 28 publications

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“…Allergens can induce mast cell activation and granule/histamine release from these cells. An immediate allergic response, which can lead to nasal symptoms, occurs within minutes after antigen exposure [ 18 , 19 , 20 , 21 ]. The ameliorative effect of DEX and Cs-4 treatments on AR is likely mediated by modulating the proinflammatory response triggered by an allergen.…”

Section: Discussionmentioning

confidence: 99%

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Anti-Inflammatory Effects of a Cordyceps sinensis Mycelium Culture Extract (Cs-4) on Rodent Models of Allergic Rhinitis and Asthma

et al. 2020

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Allergic rhinitis and asthma are common chronic allergic diseases of the respiratory tract, which are accompanied by immunoglobulin E (IgE)-mediated inflammation and the involvement of type 2 T helper cells, mast cells, and eosinophils. Cordyceps sinensis (Berk.) Sacc is a fungal parasite on the larva of Lepidoptera. It has been considered to be a health-promoting food and, also, one of the best-known herbal remedies for the treatment of airway diseases, such as asthma and lung inflammation. In the present study, we demonstrated the antiallergic rhinitis effect of Cs-4, a water extract prepared from the mycelium culture of Cordyceps sinensis (Berk) Sacc, on ovalbumin (OVA)-induced allergic rhinitis in mice and the anti-asthmatic effect of Cs-4 in a rat model of asthma. Treatment with Cs-4 suppressed the nasal symptoms induced in OVA-sensitized and challenged mice. The inhibition was associated with a reduction in IgE/OVA-IgE and interleukin (IL)-4/IL-13 levels in the nasal fluid. Cs-4 treatment also decreased airway responsiveness and ameliorated the scratching behavior in capsaicin-challenged rats. It also reduced plasma IgE levels, as well as IgE and eosinophil peroxidase levels, in the bronchoalveolar fluid. Cs-4 treatment completely suppressed the increases in IL-4, IL-5, and IL-13 levels in rat lung tissue. In conclusion, our results suggest that Cs-4 has the potential to alleviate immune hypersensitivity reactions in allergic rhinitis and asthma.

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Section: Discussionmentioning

confidence: 99%

“…The cell pellet was lysed with 0.1% Triton X. The degree of degranulation was determined by measuring the release of β-hexosaminidase, a reliable indicator of mast cell degranulation [ 20 ]. The percentage of β-hexosaminidase release = absorbance of supernatant/(absorbance of supernatant + absorbance of lysate) × 100.…”

Section: Methodsmentioning

confidence: 99%

Anti-Inflammatory Effects of a Cordyceps sinensis Mycelium Culture Extract (Cs-4) on Rodent Models of Allergic Rhinitis and Asthma

et al. 2020

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“…Histamine causes coronary vasoconstriction, cardiac depression, systemic vasodilatation and tachycardia, inhibits release of norepinephrine, chemotaxis and release of other soluble mediators by inflammatory cells during anaphylaxis (11). Another pre-formed inflammatory soluble mediator of interest was β-hexosaminidase (cumulative mean difference: 23787093.286 ng/ml) which is also found in the granules of mast cells (26). β-hexosaminidase is released at the same time as histamine and it is a typical indicator of mast cell degranulation (27).…”

Section: Discussionmentioning

confidence: 99%

Identification of Soluble Mediators in IgG-Mediated Anaphylaxis via Fcγ Receptor: A Meta-Analysis

et al. 2019

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Background: Anaphylaxis is an acute and life-threatening allergic response. Classically and most commonly, it can be mediated by the crosslinking of allergens to immunoglobulin E (IgE)- high affinity IgE receptor (FcεRI) complex found mostly on mast cells. However, there is another pathway of anaphylaxis that is less well-studied. This pathway known as the alternative pathway is mediated by IgG and its Fc gamma receptor (Fcγ). Though it was not documented in human anaphylaxis, a few studies have found that IgG-mediated anaphylaxis can happen as demonstrated in rodent models of anaphylaxis. In these studies, a variety of soluble mediators were being evaluated and they differ from each study which causes confusion in the suitability, and reliability of choice of soluble mediators to be analyzed for diagnosis or therapeutic purposes. Hence, the objective of this meta-analysis is to identify the potential soluble mediators that are involved in an IgG-mediated anaphylaxis reaction. Methods: Studies related to IgG-mediated anaphylaxis were sourced from five search engines namely PubMed, Scopus, Ovid, Cochrane Library, and Center for Agricultural Bioscience International (CABI) regardless of publication year. Relevant studies were then reviewed based on specific inclusion factors. The means and standard deviations of each soluble mediator studied were then extracted using ImageJ or Get Data Graph Digitiser software and the data were subjected to meta-analysis. Results: From our findings, we found that histamine, serotonin, platelet activating factor (PAF), β-hexosaminidase, leukotriene C4 (LTC 4 ), mucosal mast cell protease-1 (MMCP-1), interleukins (IL)-4,−6, and−13; tumor necrosis factor alpha (TNF-α), and macrophage inflammatory protein-1α (MIP-1α) were often being analyzed. Out of these soluble mediators, histamine, PAF, β-hexosaminidase, IL-6, and−13, MIP-1α and TNF-α were more significant with positive effect size and p < 0.001. As study effect was relatively small, we performed publication bias and found that there was publication bias and this could be due to the small sample size studied. Conclusion: As such, we proposed that through meta-analysis, the potential soluble mediators involved in rodent IgG-mediated anaphylaxis to be histamine, PAF, β-hexosaminidase, IL-6 and−13 and MIP-1α, and TNF-α but will require further studies with larger sample size.

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“…2.2.3 Nanoparticle-tracking analysis (NTA). Particle sizes of selected CMC-ACP dispersions were analysed using a highresolution NTA-device (NanoSight NS300, Malvern, UK) [29][30][31][32] in a light-scatter mode according to the manufacturer's soware manual (NanoSight NS300 User Manual, MAN0541-02-EN, 2018). The ideal detection threshold was determined to include as many particles as possible with the restrictions that 10-100 red crosses were counted while only <10% were not associated with distinct particles.…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Influence of molecular weight and concentration of carboxymethyl chitosan on biomimetic mineralization of collagen

et al. 2020

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A rapid and sensitive assay based on particle analysis for cell degranulation detection in basophils and mast cells

Cited by 31 publications

References 28 publications

Anti-Inflammatory Effects of a Cordyceps sinensis Mycelium Culture Extract (Cs-4) on Rodent Models of Allergic Rhinitis and Asthma

Anti-Inflammatory Effects of a Cordyceps sinensis Mycelium Culture Extract (Cs-4) on Rodent Models of Allergic Rhinitis and Asthma

Identification of Soluble Mediators in IgG-Mediated Anaphylaxis via Fcγ Receptor: A Meta-Analysis

Influence of molecular weight and concentration of carboxymethyl chitosan on biomimetic mineralization of collagen

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