Isolation of <i>Trueperella pyogenes</i> in a case of thoracic and abdominal abscess in a galago (<i>Otolemur garnettii</i>)

Salleng, Kenneth J.; Burton, Bryce; Apple, Troy M.; Sánchez, Susan

doi:10.1111/jmp.12223

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…Likewise, some other sporadic cases of infectious diseases associated with T. pyogenes were described in a galago (17), in gray slender lorises (18,19) and in a eurasian lynx (20).…”

Section: Introductionmentioning

confidence: 93%

Studies on Trueperella Pyogenes Isolated from an Okapi (Okapia johnstoni) and A Royal Python (Python Regius)

Ahmed

Alssahen

Lämmler

et al. 2020

Preprint

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Background The present study was designed to characterize phenotypically and genotypically two Trueperella pyogenes strains isolated from an okapi (Okapia johnstoni) and a royal python (Python regius), respectively.Results The species identity could be confirmed by phenotypic properties, by MALDI-TOF MS analysis and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. Furthermore, sequencing of the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), the target genes rpoB encoding the β-subunit of bacterial RNA polymerase, tuf encoding elongation factor tu and plo encoding the putative virulence factor pyolysin allowed the identification of both T. pyogenes isolates at species level.Conclosion Both strains could be clearly identified as T. pyogenes. The T. pyogenes strain isolated in high number from the vaginal discharge of an okapi seems to be of importance for the infectious process; the T. pyogenes strain from the royal python could be isolated from an apparently non-infectious process. However, both strains represent the first isolation of T. pyogenes from these animal species.

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“…Likewise, some other sporadic cases of infectious diseases associated with T. pyogenes were described in a galago (17), in gray slender lorises (18,19) and in a eurasian lynx (20).…”

Section: Introductionmentioning

confidence: 93%

Studies on Trueperella Pyogenes Isolated from an Okapi (Okapia johnstoni) and A Royal Python (Python Regius)

Ahmed

Alssahen

Lämmler

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Additionally, T. pyogenes infections were reported from a bison and from camels [13,14], from goitered gazelles [15] and from a white-tailed deer [16]. Likewise, some other sporadic cases of infectious diseases associated with T. pyogenes were described in a galago [17], in gray slender lorises [18,19] and in a eurasian lynx [20].…”

Section: Introductionmentioning

confidence: 99%

Studies on Trueperella pyogenes isolated from an okapi (Okapia johnstoni) and a royal python (Python regius)

et al. 2020

View full text Add to dashboard Cite

Background The present study was designed to characterize phenotypically and genotypically two Trueperella pyogenes strains isolated from an okapi (Okapia johnstoni) and a royal python (Python regius). Case presentation The species identity could be confirmed by phenotypic properties, by MALDI-TOF MS analysis and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. Furthermore, sequencing of the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), the target genes rpoB encoding the β-subunit of bacterial RNA polymerase, tuf encoding elongation factor tu and plo encoding the putative virulence factor pyolysin allowed the identification of both T. pyogenes isolates at species level. Conclusions Both strains could be clearly identified as T. pyogenes. The T. pyogenes strain isolated in high number from the vaginal discharge of an okapi seems to be of importance for the infectious process; the T. pyogenes strain from the royal python could be isolated from an apparently non-infectious process. However, both strains represent the first isolation of T. pyogenes from these animal species.

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Trueperella

Lämmler¹,

Sammra²,

Glaeser³

et al. 2020

Bergey's Manual of Systematics of Archaea and Bacteria

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True.pe.rel'la. N.L. fem. n. Trueperella named after Hans Georg Trüper, the German microbiologist. Actinobacteria / Actinobacteria / Actinomycetales / Actinomycetaceae / Trueperella Cells are Gram‐reaction‐positive, nonmotile, non‐spore‐forming coccobacilli and short rods that occur singly, in pairs (V, T, and palisade formations), or in clusters. Cells vary in shape and size in different media. Cells from 24‐h‐old broth cultures are Gram‐reaction‐positive but may be Gram‐reaction‐variable in older cultures. Pinpoint, in most cases β‐hemolytic colonies occur on sheep blood agar after 24 h of incubation. After 48–72 h of incubation, colonies are 0.5–1.5 mm in diameter, convex, circular, and translucent with entire edges. Aerobic and facultatively anaerobic. Strictly fermentative. Lactic acid is the primary metabolic product in glucose–yeast extract broth, but acetate and succinate are minor products. The peptidoglycan is of the type A5a, variations l ‐Lys– l ‐Ala– l ‐Lys– d ‐Glu or l ‐Lys– l ‐Lys– d ‐Glu. The muramic acid residue of the peptidoglycan is N ‐acetylated. Characteristic whole‐cell sugars are rhamnose and glucose. Mycolic acids are not present. The long‐chain fatty acids are primarily straight‐chain saturated and monounsaturated in addition to traces of branched‐chain fatty acids of the iso‐/anteiso type. The phospholipid type is type PI, comprising diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), and an unknown phosphoglycolipid (PGL) of unknown composition. The principal menaquinone is MK‐10(H4). DNA G + C content (mol%) : 56–66. Type species : Trueperella pyogenes Yassin et al. 2011 VP .

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Isolation of Trueperella pyogenes in a case of thoracic and abdominal abscess in a galago (Otolemur garnettii)

Abstract: T. pyogenes should be considered in abscesses/wounds of galagos.

Cited by 4 publications

References 10 publications

Studies on Trueperella Pyogenes Isolated from an Okapi (Okapia johnstoni) and A Royal Python (Python Regius)

Studies on Trueperella Pyogenes Isolated from an Okapi (Okapia johnstoni) and A Royal Python (Python Regius)

Studies on Trueperella pyogenes isolated from an okapi (Okapia johnstoni) and a royal python (Python regius)

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