Cell spreading and viability on zein films may be facilitated by transglutaminase

Cui, Hemiao; Liu, Gang L.; Padua, Graciela W.

doi:10.1016/j.colsurfb.2016.05.048

Cited by 13 publications

(18 citation statements)

References 39 publications

(42 reference statements)

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“…SEM data showed that ZNFs scaffolds had a high porosity structure, where the area of the pores ranged from 0.02883 to 0.0937 (µm 2 ) ±SD (Figure 1 and Table 2), which allows for excellent cell adhesion and proliferation. Moreover, zein itself was previously reported as a substrate for tissue transglutaminase which is an enzyme in cell-surface interactions by forming a bridge between integrin and fibronectin 55. The bridging between zein and tissue transglutaminase offers a platform for effective cell adhesion.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, zein offers additional potential advantages being reported as a cytocompatible protein with a good ability for the proliferation of both HL-7702 cells and NIH3T3 cells 58. Besides its cell adhesive properties, the proliferative potential of zein can be explained by provoking cell spreading which is a key process, following cell adherence, towards successful cell proliferation and growth 55…”

Section: Resultsmentioning

confidence: 99%

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<p>α-Bisabolol-Loaded Cross-Linked Zein Nanofibrous 3D-Scaffolds For Accelerating Wound Healing And Tissue Regeneration In Rats</p>

El-Lakany¹,

Abd-Elhamid²,

Kamoun³

et al. 2019

IJN

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ObjectivesNovel α-bisabolol (BIS)-loaded citric acid cross-linked zein nanofibrous scaffolds (C-ZNFs) were proposed to serve as safe platforms for promoting wound repair in rats.MethodsZNFs were synthesized using electrospinning technique, then NFs, with adequate water resistance, were produced using citric acid as a safe cross-linker.ResultsCompared to the uncross-linked ZNFs, cross-linking with 7% w/w citric acid decreased swelling index by 3 folds, while the tensile strength and the contact angle were enhanced to 2.5 and 3.8 folds, respectively. SEM images showed beads-free homogeneous NFs with a fully inter-connected 3D-network, where the average diameter of optimized C-ZNFs was 181.7±50 nm. After 24 h, C-ZNFs exhibited a decreased BIS release rate (45.6%), compared to uncross-linked mats (84.9%). By increasing BIS concentration, the cell adhesion (WI38 fibroblasts) was improved which can be attributed mainly to BIS activation of transforming growth factor-beta (TGF-β1). The MTT-OD obtained values indicated that all tested zein scaffolds significantly enhanced the viability of WI38 fibroblasts, compared to the control after 48h of incubation which can be referred to the proliferative potential of zein by provoking cell spreading process. The scratch wound assay demonstrated that BIS-loaded ZNF scaffolds showed accelerated migration and proliferation of fibroblasts expressed by significantly higher wound closure rates compared to the control sample. BIS-loaded-C-ZNFs prominently accelerated tissue regeneration for wound closure demonstrated by entirely grown epithelium with normal keratinization and rapid wound contraction, compared to the control. Immunohistochemical results confirmed the superiority of BIS-loaded-C-ZNFs, where the observed reduced NF-κB and the elevated cytokeratin expressions confirmed the anti-inflammatory and proliferative effects of the scaffolds, respectively.ConclusionIn-vitro, optimized C-ZNFs offered a satisfactory cytocompatibility, adhesion and healing which were consistent with the in-vivo results. BIS-loaded-C-ZNFs could be regarded as a promising and effective biomaterial for tissue regeneration and for accelerating the wound healing process.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

<p>α-Bisabolol-Loaded Cross-Linked Zein Nanofibrous 3D-Scaffolds For Accelerating Wound Healing And Tissue Regeneration In Rats</p>

El-Lakany¹,

Abd-Elhamid²,

Kamoun³

et al. 2019

IJN

View full text Add to dashboard Cite

show abstract

“…Among the various plant‐derived proteins, zein, a corn protein rich in glutamine (21–26%), leucine (20%), proline (9%), and alanine (8%), has shown promise as a biomaterial for bone repair (Gong, Wang, Sun, Xue, & Wang, ; Tu et al, ), having low immunogenicity (Wang et al, ; Wang, Lin, Liu, Sheng, & Wang, ), antibacterial properties (Gong et al, ), and biodegradation (Reddy & Yang, ; Wang et al, ; Wang et al, ). Although it is unclear how cells interact with zein's chemistry, a recent study identified an interaction between tissue transglutaminase, an extracellular enzyme, and zein, which may be responsible for the enhancement in cell viability (Cui, Liu, & Padua, ). Zein's unique antibacterial properties without compromising cell proliferation (Lu et al, ) may be an attractive property for bone defects that have become infected.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the cytocompatibility and differentiation of bone progenitors on electrospun zein scaffolds

Turner

Collins

Blaber

et al. 2019

J Tissue Eng Regen Med

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Bone fractures often result in complications that require surgical intervention to promote fracture healing. Tissue engineering seeks to alleviate the need for autologous bone grafting by utilizing scaffolds that can promote bone fracture healing. Plant-derived materials are desirable biomaterials because of their biodegradability, availability, and low immunogenicity. Among various plantderived proteins, zein, which is a corn protein, has shown promise for bone repair.However, when processed, zein is often blended with synthetic materials to improve mechanical properties and overall hydrolytic stability. In this study, pure zein was electrospun to create fibrous scaffolds and cross-linked with trimethylolpropane triglycidyl ether to improve hydrolytic stability. Scaffolds were characterized and evaluated in vitro for promoting the osteogenic differentiation of MC3T3-E1 cells, which are bone progenitor cells. Cross-linked zein scaffolds retained their uniform fiber morphologies after hydration. MC3T3-E1 cells grew and differentiated on the zein scaffolds even in the absence of induction factors, as demonstrated by increased alkaline phosphatase activity, mineralization, and early upregulation of Runx2 gene expression, a transcription factor associated with osteoblast differentiation. These studies demonstrate that stable, zein fibrous scaffolds could have potential for use in bone repair applications.

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“…Zein films showed good biocompatibility with liver HL-7702 cells and mice NIH3T3 fibroblasts. [19][20][21] It was suggested that zein hydrophilic surfaces exposed a larger number of glutamine residues, which may be favorable to 3T3 cell attachment. Zein showed better biocompatibility with human endothelial cells from umbilical veins than Corning plates.…”

Section: Introductionmentioning

confidence: 99%

“…It was discussed that enhanced cell adhesion possibly involved tTG-mediated cross-linking between zein and cell structural proteins. 20 The objective of this work was to investigate the attachment of MDA-MB-231 and HeLa cells, used as cancer cell surrogates, to nanostructured zein substrates. MDA-MB-231 cells are human breast cancer cells derived from metastatic sites.…”

Section: Introductionmentioning

confidence: 99%

MDA-MB-231 and HeLa cells attachment to nanostructured zein surfaces

Cui

Qin

Liu

et al. 2018

Journal of Bioactive and Compatible Polymers

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Detection and characterization of tumor cells are important for cancer diagnosis and therapy. Various surface attachment methods have been proposed for the capture and enumeration of cancer cells, including immunoaffinity and nanostructured surfaces. Zein, a corn protein, has shown good biocompatibility with human liver cells (HL-7702) and mice fibroblasts (NIH3T3). In previous work, it was found that tissue transglutaminase coated on zein substrates enhanced adhesion and spreading of NIH3T3 fibroblast cells. In the present study, cancer cell adhesion to nanostructured zein substrates was investigated. MDA-MB-231 and HeLa cells were used as cancer cell surrogates. MDA-MB-231 cell immobilization was enhanced on zein films, prepared with 80% ethanol, over glass surfaces. The application of tissue transglutaminase onto nanostructured zein substrates further increased cell spreading and adhesion. Cell immobilization increased linearly with the tissue transglutaminase content of the substrate. The effect of substrate morphology was also investigated by seeding cells on electrospun zein fibers. HeLa cell adhesion was also enhanced by zein substrates. This study provided preliminary supporting evidence for developing a zein platform for circulating tumor cells' immobilization.

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Cell spreading and viability on zein films may be facilitated by transglutaminase

Cited by 13 publications

References 39 publications

<p>α-Bisabolol-Loaded Cross-Linked Zein Nanofibrous 3D-Scaffolds For Accelerating Wound Healing And Tissue Regeneration In Rats</p>

<p>α-Bisabolol-Loaded Cross-Linked Zein Nanofibrous 3D-Scaffolds For Accelerating Wound Healing And Tissue Regeneration In Rats</p>

Evaluating the cytocompatibility and differentiation of bone progenitors on electrospun zein scaffolds

MDA-MB-231 and HeLa cells attachment to nanostructured zein surfaces

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