Light-sheet imaging of mammalian development

Medeiros, Gustavo de; Balint, Balázs; Hufnagel, Lars

doi:10.1016/j.semcdb.2015.11.001

Cited by 28 publications

(17 citation statements)

References 59 publications

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“…As a result, a tight PBX-dependent regulation of individual target genes and gene networks requires the formation of different context-specific combinatorial complexes that guide distinct developmental programs, a process poorly understood. As high-throughput technology (for reviews, see Spitz and Furlong 2012;Villar et al 2014;Denker and de Laat 2016), live imaging (for reviews, see Saiz et al 2015;de Medeiros et al 2016), single-cell transcriptomics (see Sahakyan and Plath 2016;Regev et al 2017), and system-level approaches (Du et al 2014;Regev et al 2017) are brought to bear, PBX-dependent networks and molecular circuitries will be illuminated in specific morphogenetic contexts and in different cell types at different developmental stages. Ultimately, the availability of encyclopedias of regulatory elements Yue et al 2014) in the genome of different species, together with tissue-specific and temporally controllable LOF animal models to guide functional studies in vivo, will enable a deeper understanding of how various organisms use combinations of regulatory factors and pathways to assume all of the beautiful forms that they display.…”

Section: Concluding Remarks and Perspectivesmentioning

confidence: 99%

‘Building a perfect body’: control of vertebrate organogenesis by PBX-dependent regulatory networks

2019

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Pbx genes encode transcription factors that belong to the TALE (three-amino-acid loop extension) superclass of homeodomain proteins. We have witnessed a surge in information about the roles of this gene family as leading actors in the transcriptional control of development. PBX proteins represent a clear example of how transcription factors can regulate developmental processes by combinatorial properties, acting within multimeric complexes to implement activation or repression of transcription depending on their interaction partners. Here, we revisit long-emphasized functions of PBX transcription factors as cofactors for HOX proteins, major architects of the body plan. We further discuss new knowledge on roles of PBX proteins in different developmental contexts as upstream regulators of Hox genes-as factors that interact with non-HOX proteins and can work independently of HOX-as well as potential pioneer factors. Committed to building a perfect body, PBX proteins govern regulatory networks that direct essential morphogenetic processes and organogenesis in vertebrate development. Perturbations of PBX-dependent networks can cause human congenital disease and cancer.

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Section: Concluding Remarks and Perspectivesmentioning

confidence: 99%

‘Building a perfect body’: control of vertebrate organogenesis by PBX-dependent regulatory networks

2019

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“…Lightsheet microscopy combines high-speed acquisition with low phototoxicity and good optical sectioning at subcellular resolution. Tracking evolving biological processes over a long-term timescale (in days) and using high-speed recordings (seconds or minutes) can bridge different spatial and temporal scales (de Medeiros et al, 2016;Höckendorf et al, 2012). Indeed, light-sheet imaging has been applied to the study of calcium dynamics in plants (Costa et al, 2013), clathrin dynamics, organelle reorganization and cell migration in zebrafish (Liu et al, 2018), division dynamics in tumor spheroids (Lorenzo et al, 2011), mouse intestinal organoid development (Serra et al, 2019) and mouse embryo development, both from pre-implantation embryos (Strnad et al, 2016) and from gastrulation to organogenesis with single cell resolution (McDole et al, 2018).…”

Section: Temporally Resolved Single Cell Methodsmentioning

confidence: 99%

Exploring single cells in space and time during tissue development, homeostasis and regeneration

2019

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Complex 3D tissues arise during development following tightly organized events in space and time. In particular, gene regulatory networks and local interactions between single cells lead to emergent properties at the tissue and organism levels. To understand the design principles of tissue organization, we need to characterize individual cells at given times, but we also need to consider the collective behavior of multiple cells across different spatial and temporal scales. In recent years, powerful single cell methods have been developed to characterize cells in tissues and to address the challenging questions of how different tissues are formed throughout development, maintained in homeostasis, and repaired after injury and disease. These approaches have led to a massive increase in data pertaining to both mRNA and protein abundances in single cells. As we review here, these new technologies, in combination with in toto live imaging, now allow us to bridge spatial and temporal information quantitatively at the single cell level and generate a mechanistic understanding of tissue development.

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“…Due to the excitation with a thin plane of light it has the advantage of fast acquisition and weak perturbation of the sample [4]. The gentle light excitation allows in vivo imaging over long periods of time [5]. Combined with an arrangement providing an extended depth of field, a large volumetric field of view can be explored at high speed [6].…”

Section: Light Sheet Fluorescence Microscopy (Lsfm) Is a Non-destructmentioning

confidence: 99%

Fabrication of YAG/Cr:YAG transparent composite ceramics and characterization by light sheet fluorescence imaging

Boulesteix¹,

Perrière²,

Maı̂tre³

et al. 2019

Optical Materials

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Transparent composite ceramics based on YAG/Cr:YAG bilayer samples were manufactured by the solid-state reaction route and sequential slip-casting technique. They were characterised by light sheet fluorescence imaging (LSFI). This work shows that LSFI allows determining the spatial distribution of chromium with different valence state (Cr 4+ and Cr 3+) with a resolution close to 5 µm and high sensitivity in regards to the concentration. LSFI thus appears well adapted to the characterisation of transparent ceramic materials with luminescent dopant gradient used as solid-state lasers optical components.

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Light-sheet imaging of mammalian development

Cited by 28 publications

References 59 publications

‘Building a perfect body’: control of vertebrate organogenesis by PBX-dependent regulatory networks

‘Building a perfect body’: control of vertebrate organogenesis by PBX-dependent regulatory networks

Exploring single cells in space and time during tissue development, homeostasis and regeneration

Fabrication of YAG/Cr:YAG transparent composite ceramics and characterization by light sheet fluorescence imaging

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