Development of a Triplet–Triplet Absorption Ruler: DNA- and Chromatin-Mediated Drug Molecule Release from a Nanosurface

Abstract

Triplet-triplet (T-T) absorption spectroscopy has been used successfully as a molecular ruler to understand the actual release process of sanguinarine as a drug molecule from a gold nanoparticle surface in the presence of cell components, that is, DNA and chromatin. The obtained results have been verified by fluorescence and surface-enhanced Raman spectroscopy (SERS), and a plausible explanation has been put forward to describe the underestimation and overestimation of the percentage (%) of the release of drug molecules measured by fluorescence- and SERS-based techniques, respectively, over the highlighted T-T absorption spectroscopy. Because of the intrinsic nature of absorption, the reported T-T absorption spectroscopic assay overpowers fluorescence- and SERS-based assays, which are limited by the long-range interaction and nonlinear dependence of the concentration of analytes, respectively.

Metal nanoparticle alters adenine induced charge transfer kinetics of vitamin K3 in magnetic field

“…The UV–visible absorption of 2.8 μg/ml of AuNP (olive line) and FeNP (black line) have been shown in Fig. 1 a with the characteristic surface plasmon band for AuNP and a gradually decreasing absorption for the FeNP as reported in the literature 55 , 56 . The addition of AuNP or FeNP to the complex MQ-ADN shows a decrease in the 260 nm peak with a concomitant increase in the peaks over the red end, with an isosbestic point at 280 nm (Fig.…”

“…Spherical Au nanoparticles (AuNP) are synthesized by the TSC-based method reported earlier 55 . Briefly, a three-necked round bottom flask bottle fitted with a cold-water jacket condenser is filled with 100 ml of water in which 1.25 ml of 10 −2 M aqueous solution of HAuCl 4 is added to it.…”

“…The studies of magnetic field effect (MFE) with nanomaterials have been reported earlier by E. Cohen 54 . Das Chakroborty et al showed the occurrence of H-abstraction in the presence of AuNP and FeNP with sanguinarine, which acts as a toxin that kills animal cells through its action on the Na + /K + -ATPase transmembrane protein 55 , 56 . A highly directional MF can be generated by a single domain superparamagnetic Fe 3 O 4 nano surface in the presence of a low external MF (0.08 T) on the nano surface adsorbed molecules.…”

Wide Range Morphological Transition of Silver Nanoprisms by Selective Interaction with As(III): Tuning–Detuning of Surface Plasmon Offers To Decode the Mechanism

“…Synthesis of AgNPr. The initial triangular AgNPrs were synthesized by following Mirkin's method 60 with slight modification. Briefly, 25 mL of Milli-Q water was taken in a 50 mL beaker, and then 250 μL of 10 −2 M AgNO 3 was added drop by drop in it.…”

Gigantol from Dendrobium chrysotoxum Lindl. binds and inhibits aldose reductase gene to exert its anti-cataract activity: An in vitro mechanistic study

“…In this experiment, the SERS peaks of AR gene were greatly enhanced with the increasing amounts gigantol. The mechanism of enhancement is ascribed to the appearance of resonance between AR gene and gigantol, based on the fact that the resonance enhancement should provide several orders of additional magnitude enhancement relative to the electromagnetic enhancement alone (Zhou H et al, 2015;Chakraborty SD et al, 2016).…”