Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress

Abstract

Peanuts (Arachis hypogaea L.) are a major source of plant protein grown in subtropical and tropical regions of the world. Pre-harvest aflatoxin contamination is a major problem that affects peanut crop yield and food safety. Poor understanding of molecular and cellular mechanisms associated with aflatoxin resistance is largely responsible for the lack of progress in elucidating a process/methodology for reducing aflatoxin contamination in peanuts. Drought perturbs the invasion of the aflatoxin producing fungus and thus affects the quality and yield of peanut. Therefore, more studies involving the effects of drought stress to determine the molecular changes will enhance our understanding of the key metabolic pathways involved in the combined stresses. The changes associated with the biotic and abiotic interactions within the peanut will be used to determine the metabolic pathways involved in the stress tolerance. This research would be beneficial in identifying the tolerant molecular signatures and promoting food safety and consumer health through breeding superior quality peanut cultivars.

The disadvantages of being a hybrid during drought: A combined analysis of plant morphology, physiology and leaf proteome in maize

“…g ., [99, 102–103]). Others have confirmed a drought-induced elevation of their levels for drought-tolerant genotypes but observed decreased amounts of these proteins in drought-susceptible genotypes [104], or a decrease in plants showing a higher susceptibility to water deficiency and no changes in plants that are resistant to this stressor [96]. There are also studies reporting a down-regulation of LHC proteins both in drought-resistant and sensitive genotypes [112, 114] or an increase of levels of some LHC proteins, a decrease of others in a genotype with a lower susceptibility to drought conditions, but no changes in a highly susceptible genotype [119].…”

“…Various authors have described a decrease in the levels of this protein in the leaves of some drought-sensitive genotypes but an increase, no changes or at least a more gradual decrease in a corresponding drought-resistant genotype [98–99, 104, 107, 112–114]. However, others did not find genotypic differences in the drought-induced changes in this OEC component [101, 115–116].…”

“…Katam et al . [104] reported an increased level of the A isozyme of chloroplastic GAPDH in drought-stressed plants of groundnut for a tolerant genotype but an absence of this form in leaves of a sensitive genotype, which means that there can be at least interspecific differences in this respect.…”

Proteomic analysis of tea plants (Camellia sinensis) with purple young shoots during leaf development

“…Functional annotation of proteins was conducted using Gene Ontology (GO) annotation [25]. The differentially accumulated proteins were assigned to the Cluster of Orthologous Groups of proteins (COG) database and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database [26].…”

A Proteomic View on the Role of Legume Symbiotic Interactions

“…For instance, analyses of peanut ( Arachis hypogaea L.) varieties with contrasting tolerance to water deficit have shown a relation between plant tolerance to the stress and the abundance of proteins involved in stress signaling and wax biosynthesis in leaves (Kottapalli et al, 2009). A similar approach was taken by Katam et al (2016) who identified proteins related to nitrogen metabolism, defense and cellular biogenesis exclusively in the tolerant peanut cultivar. Abiotic stress responses in a major legume crop like soybean have been reviewed elsewhere (Hossain et al, 2012, 2013; Hossain and Komatsu, 2014; Komatsu et al, 2015).…”