KOTMIN13, a Korean herbal medicine alleviates allergic inflammation in vivo and in vitro

Lee, Eujin; Kim, Sun-Gun; Park, Na-Young; Park, Hyo-Hyun; Jeong, Kyu-Tae; Choi, Joon‐Hwan; Lee, In-Hae; Lee, Hwa-Dong; Kim, Keuk‐Jun; Lee, Eunkyung

doi:10.1186/s12906-016-1155-4

Cited by 9 publications

(7 citation statements)

References 34 publications

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“…Yoo et al demonstrated that aged black garlic extract inhibited the release of β-hexosaminidase on HumRBL-2H3 cells [52]. Moreover, Lee et al showed that the Korean herbal medicine KOTMIN13 inhibited the degranulation of bone marrow mast cells derived [53]. Our results demonstrated that methanolic LE inhibits the degranulation of both HumRBL-2H3 and LAD2 cells in response to immunological and non-immunological stimuli, respectively.…”

Section: Discussionsupporting

confidence: 61%

Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function

et al. 2019

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Allium genus plants, such as leek (Allium porrum), are rich sources of anti-inflammatory and anti-oxidant secondary metabolites; this is of interest because it demonstrates their suitability as pharmacological alternatives for inflammatory processes, including allergy treatment. The composition of methanolic leek extract (LE) was analyzed by GC–MS and LC–IT/MS, and the total phenolic content and antioxidant capacity were quantified by colorimetric methods. Its pharmacological potential was analyzed in human bronchial epithelial Calu-3 cells, human mast cells LAD2, and humanized rat basophiles RBL-2H3. LE exhibited a cytotoxic effect on Calu-3 cells and HumRBL-2H3 cells only at high concentrations and in a dose-dependent manner. Moreover, LE decreased the degranulation of LAD2 and HumRBL-2H3 cells. LE treatment also significantly prevented alterations in transepithelial electrical resistance values and mRNA levels of glutathione-S-transferase (GST), c-Jun, and NFκB after treatment with H2O2 in ALI-cultured Calu-3 cells. Finally, ALI-cultured Calu-3 cells treated with LE showed lower permeability to Ole e 1 compared to untreated cells. A reduction in IL-6 secretion in ALI-cultured Calu-3 cells treated with LE was also observed. In summary, the results obtained in this work suggest that A. porrum extract may have potential anti-allergic effects due to its antioxidant and anti-inflammatory properties. This study provides several important insights into how LE can protect against allergy.

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Section: Discussionsupporting

confidence: 61%

Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function

et al. 2019

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“…(Allii Macrostemonis Bulbus). A. macrostemon , a major constituent of JHGT, has been reported to have a therapeutic effect on acute myocardial ischemia via reducing inflammatory responses [ 37 ]. Furthermore, A. macrostemon and T. kirilowii are among the top 10 natural products for the treatment of coronary artery diseases [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Literature‐Based Drug Repurposing in Traditional Chinese Medicine: Reduced Inflammatory M1 Macrophage Polarization by Jisil Haebaek Gyeji‐Tang Alleviates Cardiovascular Disease In Vitro and Ex Vivo

Lee

Kim

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Relatively high proportions of proinflammatory M1-like macrophages in tissues may lead to vascular impairment and trigger numerous diseases including atherosclerosis-related cardiovascular disease (CVD). Jisil Haebaek Gyeji-tang (JHGT), a polyherbal decoction, is traditionally used to treat various human ailments including chest pain, angina, and myocardial infarction. In the present study, we investigated the anti-inflammatory effects of JHGT on lipopolysaccharide- (LPS-) stimulated M1 macrophage polarization generated via the mitogen-activated protein kinases (MAPKs) pathway in RAW 264.7 mouse macrophages. The reducing power of JHGT was also investigated using DAF-FA DA in a zebrafish model. JHGT significantly reduced inflammatory mediator levels, including iNOS, COX2, TNF-α, IL-6, and IL-1β, as compared with LPS-stimulated controls in vitro and ex vivo. Furthermore, JHGT suppressed the ERK1/2, JNK, and p38 MAPK pathways and reduced p-IκBα levels and the nuclear translocation of NF-κB in RAW 264.7 cells. In addition, treatment with JHGT significantly reduced the NO levels in LPS-treated zebrafish larva ex vivo. Our findings show the potent anti-inflammatory properties of JHGT are due to its suppression of MAPK signaling, NF-κB translocation, and M1 macrophage polarization.

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“…These findings were supported by the observed amelioration of various indicators of inflammation in the OVA-induced asthma model mice, which included the weights of immune organs, the number of leukocytes released into the BALF, serum IgE concentration, thickness of the respiratory epithelium, mucus score, and Th2 cytokine and COX-2 expression. These indicators of lung inflammation have been applied in previous studies to investigate the therapeutic effects of various herbal medicines, including Phillinus linteus extract ( 38 ), Korean ginseng ( 39 ) and KOTMIN13 ( 40 ), in OVA-induced asthma models.…”

Section: Discussionmentioning

confidence: 99%

Saponin-enriched extract of Asparagus cochinchinensis alleviates airway inflammation and remodeling in ovalbumin-induced asthma model

Sung

Lee

Kim

et al. 2017

International Journal of Molecular Medicine

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Asthma is a chronic inflammatory disease characterized by T-lymphocyte and eosinophil infiltration, mucus overproduction and airway hyper-responsiveness. The present study examined the therapeutic effects and action mechanism of a saponin-enriched extract of Asparagus cochinchinensis (SEAC) on airway inflammation and remodeling in an ovalbumin (OVA)-induced asthma model. To accomplish this, alterations of the nitric oxide (NO) level, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression levels, as well as variations in immune cell numbers, immunoglobulin E (IgE) concentration, histopathological structure and inflammatory cytokine levels were measured in lipopolysaccharide (LPS)-activated RAW264.7 cells or an OVA-induced mouse model of asthma treated with SEAC. The concentration of NO and mRNA levels of COX-2 and iNOS were significantly decreased in the SEAC + LPS-treated RAW264.7 cells compared with the vehicle + LPS-treated RAW264.7 cells. Additionally, in the OVA-induced asthma model, the number of immune cells in the bronchoalveolar lavage fluid, the concentration of OVA-specific IgE, the infiltration of inflammatory cells, the bronchial thickness and the levels of the inflammatory mediators interleukin-4 (IL-4), IL-13 and COX-2 were significantly lower in the OVA + SEAC-treated group compared with the OVA + vehicle-treated group. In addition, a significant reduction in goblet cell hyperplasia, peribronchiolar collagen layer thickness and VEGF expression for airway remodeling was detected in the OVA + SEAC-treated group compared with the OVA + vehicle-treated group. These findings indicate that SEAC is a suppressor of airway inflammation and remodeling, and may therefore be useful as an anti-inflammatory drug for the treatment of asthma.

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KOTMIN13, a Korean herbal medicine alleviates allergic inflammation in vivo and in vitro

Cited by 9 publications

References 34 publications

Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function

Allium porrum Extract Decreases Effector Cell Degranulation and Modulates Airway Epithelial Cell Function

Literature‐Based Drug Repurposing in Traditional Chinese Medicine: Reduced Inflammatory M1 Macrophage Polarization by Jisil Haebaek Gyeji‐Tang Alleviates Cardiovascular Disease In Vitro and Ex Vivo

Saponin-enriched extract of Asparagus cochinchinensis alleviates airway inflammation and remodeling in ovalbumin-induced asthma model

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