Development and evaluation of a double antibody sandwich ELISA for the detection of human sDC-SIGN

Chen, Shang-Liang; Li, Yanli; Tang, Yuan; Chen, Zhicheng; Zhou, Jing; Zhou, Jia; Xiao, Lü; Zhao, Na; Chen, Zhengliang; Zuo, Daming

doi:10.1016/j.jim.2016.05.011

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…It is more sensitive when different class antibodies were used as the detection and capture antibodies with a broad spectrum of epitopes. Furthermore, the detection of multiple epitopes offers better sensitivity for detecting proteins that are present in low concentrations in a sample [11,12]. The DAS ELISA was established to determine the effective antigen yields in inactivated vaccines and thus represents an alternative for assessing the potency of FMD vaccines in vitro [13,14].…”

Section: Discussionmentioning

confidence: 99%

Development of a Double-Antibody Sandwich ELISA for Rapid Detection of the MCP Antigen Concentration in Inactivated ISKNV Vaccines

Liang

Li-xi

et al. 2021

Vaccines

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Infectious spleen and kidney necrosis virus (ISKNV) resulted in severe systemic diseases with high morbidity and mortality in Siniperca chuatsi. Vaccination is the primary method for effective prevention and control of these diseases. The development of inactivated ISKNV vaccines made some progress, but the technique of quality evaluation is scarce. Herein, a measurement of the MCP (major capsid protein) antigen concentration for the inactivated ISKNV vaccine was developed by double-antibody sandwich ELISA. Firstly, mouse monoclonal antibodies against ISKNV particles and MCP were generated. Then, a double-antibody sandwich ELISA was developed using the monoclonal antibody 1C8 1B9 as the capture antibody and Biotin-3B12 6B3 as the detection antibody. A standard curve was generated using the MCP concentration versus OD value with the linear range of concentration of 4.69~300 ng/mL. The assay sensitivity was 0.9 ng/mL. The antigen content of three batches of inactivated ISKNV vaccines was quantitatively detected using the double-antibody sandwich ELISA. The results showed that MCP antigen contents of inactivated ISKNV vaccines were positively correlated with the viral titers. The newly established double-antibody sandwich ELISA provided a useful tool for the detection of antigen quality for ISKNV inactivated vaccines.

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Section: Discussionmentioning

confidence: 99%

Development of a Double-Antibody Sandwich ELISA for Rapid Detection of the MCP Antigen Concentration in Inactivated ISKNV Vaccines

Liang

Li-xi

et al. 2021

Vaccines

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“…[16][17][18] Currently, ELISA kits of diagnosis AMI from different companies can give different results, in part because they lack standard antibodies that are directed at standard epitopes on cTnI. [16][17][18] Currently, ELISA kits of diagnosis AMI from different companies can give different results, in part because they lack standard antibodies that are directed at standard epitopes on cTnI.…”

Section: Discussionmentioning

confidence: 99%

“…Sandwich ELISA assays are often used for hospital detection because of their specificity. [16][17][18] Currently, ELISA kits of diagnosis AMI from different companies can give different results, in part because they lack standard antibodies that are directed at standard epitopes on cTnI. 19 Another study showed that the N-terminal and C-terminal portions of cTnI (approximately amino acid 1-30 and 110-210) are easy to degrade.…”

Section: Discussionmentioning

confidence: 99%

Two desired epitopes of cTnI benefit for preparation of standardized monoclonal antibodies

Yan

Yang

et al. 2019

Chirality

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Acute myocardial infarction (AMI) is one of the most severe cardiovascular diseases in humans, often resulting in unexpected death. Early detection is critical for patient survival. Sandwich ELISA is a common method for the detection of AMI. However, ELISA kits from different manufacturers can give different results, in part because of the lack of standardized epitopes. Therefore, the purpose of this study was to find two standardized epitopes. We predicted two antigen epitopes and respectively immunize mice to manufacture standardized monoclonal antibodies. Eight monoclonal antibodies were prepared. Monoclonal antibodies 7D2 and 2C3 were selected with high affinity, and their characteristics were explored. The results show that monoclonal antibodies 7D2 and 2C3 can both bind to various modified forms and complexes of cardiac troponin I (cTnI), were not cross-reaction with related antigens of normal human serum and can be paired. Therefore, we deem epitopes 30 to 42 and 77 to 89 standardized epitopes.

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Nur77 suppression facilitates androgen deprivation-induced cell invasion of prostate cancer cells mediated by TGF-β signaling

Sun

Yang

et al. 2018

Clin Transl Oncol

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Development and evaluation of a double antibody sandwich ELISA for the detection of human sDC-SIGN

Cited by 4 publications

References 19 publications

Development of a Double-Antibody Sandwich ELISA for Rapid Detection of the MCP Antigen Concentration in Inactivated ISKNV Vaccines

Development of a Double-Antibody Sandwich ELISA for Rapid Detection of the MCP Antigen Concentration in Inactivated ISKNV Vaccines

Two desired epitopes of cTnI benefit for preparation of standardized monoclonal antibodies

Nur77 suppression facilitates androgen deprivation-induced cell invasion of prostate cancer cells mediated by TGF-β signaling

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