Low‐density lipoprotein receptor‐related protein 1 is a novel modulator of radial glia stem cell proliferation, survival, and differentiation

Safina, Dina; Schlitt, Frederik; Romeo, Ramona; Pflanzner, Thorsten; Pietrzik, Claus U.; Narayanaswami, Vasanthy; Edenhofer, Frank; Faissner, Andréas

doi:10.1002/glia.23009

Cited by 45 publications

(65 citation statements)

References 108 publications

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“…Cell proliferation, migration, differentiation and survival are some of activities that LRP1 modulates in radial glia stem cells. Interestingly, in CNS, LRP1 binds apoE/Aβ complexes and regulates their clearance in the blood–brain barrier, and it is a major Aβ clearance receptor in cerebral vascular smooth muscle cells . Despite the role played by this receptor in CNS, data in microglia are still relatively scant .…”

Section: Discussionmentioning

confidence: 99%

Plasma Membrane Protein Profiling in Beta‐Amyloid‐Treated Microglia Cell Line

et al. 2017

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In the responsiveness of microglia to toxic stimuli, plasma membrane proteins play a key role. In this study we treated with a synthetic beta amyloid peptide murine microglial cells metabolically differently labelled with stable isotope amino acids (SILAC). The plasma membrane was selectively enriched by a multi-stage aqueous two-phase partition system. We were able to identify by 1D-LC-MS/MS analyses 1577 proteins, most of them are plasma membrane proteins according to the Gene Ontology annotation. An unchanged level of amyloid receptors in this data set suggests that microglia preserve their responsiveness capability to the environment even after 24-h challenge with amyloid peptides. On the other hand, 14 proteins were observed to change their plasma membrane abundance to a statistically significant extent. Among these, we proposed as reliable biomarkers of the inflammatory microglia phenotype in AD damaged tissues MAP/microtubule affinity-regulating kinase 3 (MARK3), Interferon-induced transmembrane protein 3 (IFITM3), Annexins A5 and A7 (ANXA5, ANXA7) and Neuropilin-1 (NRP1), all proteins known to be involved in the inflammation processes and in microtubule network assembly rate.

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Section: Discussionmentioning

confidence: 99%

Plasma Membrane Protein Profiling in Beta‐Amyloid‐Treated Microglia Cell Line

et al. 2017

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“…LRP1(low-density lipoprotein receptor-related protein-1) is an endocytic receptor that plays important role in binding and internalization of a wide range of biological ligands [13]. It functions in various cellular activities including proliferation, migration and differentiation of cell as well as activation of lysosomal enzymes [13,14]. It has a potential role in phagocytosis, which is necessary for removal of infectious agents [13], thus improves health condition through elimination of infection.…”

Section: Introductionmentioning

confidence: 99%

Association of Plasma Low-Density Lipoprotein Receptor-Related Protein-1 (LRP1) with Undernutrition: A Case-Control Study in Bangladeshi Adults

Hasan

Fahim

Das

et al. 2020

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Background: Studies revealed that LRP1 plays pivotal role in adipogenesis, whereas silencing of LRP1 expression can cause inhibition of adipogenesis in animal model and contribute to reduced body size. Therefore a reduction in the level of adipocytic LRP1 might have a role on weight loss. But there is no study that has explored the role of LRP1 on weight loss of human adults. However, it is not feasible to examine the role of adipocytic LRP1 on weight loss due to invasive collection procedure of adipose tissue. But plasma LRP1 can fill that void as it is a biomarker of adipocytic LRP1. Therefore, the aim of this study was to investigate the relationship of plasma low-density lipoprotein receptor-related protein-1 (LRP1) with undernutrition.Methods: A total of 270 Bangladeshi slum-dwelling adults (135 cases, BMI < 18.5 kg/m2 and 135 controls, BMI 18.5-24.9 kg/m2) were enrolled. Their socio-economic, demographic, anthropometric and biomedical data were collected. Plasma LRP1, C-reactive protein (CRP), alpha-1 acid glycoprotein (AGP), and ferritin levels were measured by ELISA, hemoglobin by Hemocue and zinc by atomic absorption spectrometry.Results: The median (IQR) values of plasma LRP1, CRP, AGP, Hemoglobin, zinc and ferritin were 1673.1 (1382.5- 1886.2) ng/ml, 2.1 (0.9 – 4.4) mg/L, 75.8 (62.9 – 95.9) mg/dL, 13.2 (12 – 14.3) g/dL, 0.74 (0.7 – 0.8) mg/L, 59.9 (26.4 – 123.2) ng/mL in healthy participants and 707.7 (588.6- 839.9) ng/ml, 0.8 (0.2 – 1.7) mg/L, 64.7 (52.9 – 86.9) mg/dL, 12.4 (11.4 – 13.4) g/dL, 0.71 (0.6 – 0.8) mg/mL, 43.7 (19.9 – 80.2) ng/mL in undernourished participants, respectively. A strong positive correlation (r = 0.70, p <0.05) between LRP1 and body mass index (BMI) was found. Multivariable logistic regression analysis revealed a positive association between low plasma LRP1 (Adj. OR= 0.98, CI = 0.98, 0.99 and p < 0.05) and undernutrition. Conclusions: The study found that increased level of LRP1 is associated with increased BMI, whereas lower level can contribute to low BMI.

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“…Unlike the brain, we report significant differences in FORSE-1 expression in the diencephalon and spinal cord. However, analogous nestin localisation expression patterns have been demonstrated around the central canal of the spinal cord during gliogenesis using LeX clones 487 and 5750 with diffuse white matter labelling from E15.5 using 5750 and earlier with 487 (Karus et al 2013;Safina et al 2016). Diffuse white matter labelling was not present with FORSE-1 until postnatal ages, as shown with 487 and 5750 at embryonic ages, likely reflecting the delayed diffusion of FORSE-1 LeX carriers (possibly phosphacan) into the extracellular space.…”

Section: Discussionmentioning

confidence: 56%

“…; Safina et al. ). Diffuse white matter labelling was not present with FORSE‐1 until postnatal ages, as shown with 487 and 5750 at embryonic ages, likely reflecting the delayed diffusion of FORSE‐1 LeX carriers (possibly phosphacan) into the extracellular space.…”

Section: Discussionmentioning

confidence: 98%

The distribution of the proteoglycan FORSE‐1 in the developing mouse central nervous system

et al. 2018

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Glycosylation is a major post‐translational modification in which a carbohydrate known as a glycan is enzymatically attached to target proteins which regulate protein folding and stability. Glycans are strongly expressed in the developing nervous system where they play multiple roles during development. The importance of these glycan epitopes in neural development is highlighted by a group of conditions known as congenital disorders of glycosylation which lead to psychomotor difficulties, mental retardation, lissencephaly, microencephaly and epilepsy. One of these glycan epitopes, known as Lewis X, is recognised by the FORSE‐1 antibody and is regionally expressed in the developing nervous system. In this study, we report the regional and temporal expression patterns of FORSE‐1 immunolabelling during the periods of neurogenesis, gliogenesis and axonogenesis in developing mouse nervous system. We demonstrate the localisation of FORSE‐1 on subsets of neuroepithelial cells and radial glial cells, and in compartments corresponding to axon tract formation. These spatial, temporal and regional expression patterns are suggestive of roles in the determination of different cell lineages and in the patterning of white matter during development, and help provide insights into the neuroanatomical regions affected by congenital disorders of glycosylation.

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Low‐density lipoprotein receptor‐related protein 1 is a novel modulator of radial glia stem cell proliferation, survival, and differentiation

Cited by 45 publications

References 108 publications

Plasma Membrane Protein Profiling in Beta‐Amyloid‐Treated Microglia Cell Line

Plasma Membrane Protein Profiling in Beta‐Amyloid‐Treated Microglia Cell Line

Association of Plasma Low-Density Lipoprotein Receptor-Related Protein-1 (LRP1) with Undernutrition: A Case-Control Study in Bangladeshi Adults

The distribution of the proteoglycan FORSE‐1 in the developing mouse central nervous system

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