Scintigraphic Tracking of Allogeneic Mesenchymal Stem Cells in the Distal Limb After Intra‐Arterial Injection in Standing Horses

Espinosa, Patricia; Spriet, Mathieu; Solé, Albert Ariza; Walker, Naomi J.; Vaughan, Betsy; Galuppo, Larry D.

doi:10.1111/vsu.12485

Cited by 12 publications

(14 citation statements)

References 23 publications

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“…In horses, partial venous thrombosis (typically subclinical) has been observed following regional i.a. catheterisation [165,166]. Another study showed severe arterial thrombosis following tourniquet application and i.a.…”

Section: Intra-arterialmentioning

confidence: 99%

“…catheterisation [165,166]. Tourniquet use was thought to be the cause and more recent studies without the tourniquet report no severe complications [165,166,168]. delivery of mesenchymal stem cells that caused severe lameness and dermal necrosis [167].…”

Section: Adverse Eventsmentioning

confidence: 99%

“…delivery of mesenchymal stem cells that caused severe lameness and dermal necrosis [167]. Tourniquet use was thought to be the cause and more recent studies without the tourniquet report no severe complications [165,166,168]. Other potential complications of i.a.…”

Section: Intra-arterialmentioning

confidence: 99%

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Use of contrast media in computed tomography and magnetic resonance imaging in horses: Techniques, adverse events and opportunities

Nelson

Goodrich

Barrett

et al. 2017

Equine Veterinary Journal

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The use of contrast media in computed tomography (CT) and magnetic resonance imaging (MRI) is increasing in horses. These contrast-enhanced imaging techniques provide improved tissue delineation and evaluation, thereby expanding diagnostic capabilities. While generally considered safe, not all contrast media exhibit the same safety profiles. The safety of contrast media use and descriptions of adverse events occurring in horses are sparsely reported. This review summarises the reported evidence of contrast media use and adverse events that occur in horses, with added contribution from other veterinary species and studies in man for comparison. This comprehensive data set empowers equine clinicians to develop use and monitoring strategies when working with contrast media. Finally, it summarises the current state-of-the-art and highlights the potential applications of contrast-enhanced CT and MRI for assessment of diseased or injured equine tissues, as well as (patho)physiological processes.

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Section: Intra-arterialmentioning

confidence: 99%

Section: Adverse Eventsmentioning

confidence: 99%

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Use of contrast media in computed tomography and magnetic resonance imaging in horses: Techniques, adverse events and opportunities

Nelson

Goodrich

Barrett

et al. 2017

Equine Veterinary Journal

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“…The MSCs were labelled as previously described [10]. Briefly, hexamethylpropyleneamine oxime (HMPAO) k was solubilised in 20 lL Dimethyl sulfoxide (DMSO) l and then diluted to 125 lg/mL with sodium chloride injection solution.…”

Section: Mesenchymal Stem Cell Labeling Techniquementioning

confidence: 99%

“…Intra-arterial injections without a tourniquet resulted in better distribution and better persistence of the MSCs compared with intravenous regional limb perfusion [9]. Intraarterial injections of the front limbs were initially performed under anaesthesia but a technique has now been established in the standing horse [10,11]. This technique is now used for treatment of soft tissue lesions from the level of the suspensory origin to the distal limb.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound‐guided injection of the cranial tibial artery for stem cell administration in horses

Torrent

Spriet

Espinosa‐Mur

et al. 2019

Equine Veterinary Journal

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Summary Background A technique for intra‐arterial injection of mesenchymal stem cells (MSC) has been established for front limbs with the use of the median artery. This approach has been proposed for treatment of soft tissue injuries of the equine distal limb. A technique has not been validated yet for hindlimb injection. Objectives To assess the feasibility of injection of the cranial tibial artery in horses, and to evaluate the distribution and persistence of MSC after injection. Study design In vivo experiment. Methods In a first phase, the cranial tibial arteries of both hindlimbs of three research horses were catheterised with ultrasound guidance under general anaesthesia and injected with iodinated contrast. In the second phase, iodinated contrast was injected in three standing sedated horses with ultrasound guidance. In the final phase, 99mTechnetium‐HMPAO labelled allogenic bone marrow derived equine MSC were injected under standing sedation with the same technique in three other horses. Scintigraphy was used to assess MSC distribution and persistence for 24 h. Ultrasound was performed 24 h after injection to assess vessel impairment. Results Arterial injection was achieved in all 18 limbs without any significant complications. Mild partial periarterial injection was observed in four limbs. Scintigraphic images demonstrated diffuse MSC distribution from the tarsal area to the foot. Persistence decreased over time but signal was still present at 24 h. Main limitations Limited retention of the radiolabel in the MSC. Conclusions Ultrasound‐guided injection of the cranial tibial artery can be performed both under general anaesthesia and standing sedation in horses. This technique could be used for MSC treatment of equine proximal suspensory desmopathy or other injuries in the distal hindlimb.

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Principles of Therapy for Lameness

Koch¹,

Goodrich²,

Smanik³

et al. 2020

Adams and Stashak's Lameness in Horses

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Scintigraphic Tracking of Allogeneic Mesenchymal Stem Cells in the Distal Limb After Intra‐Arterial Injection in Standing Horses

Cited by 12 publications

References 23 publications

Use of contrast media in computed tomography and magnetic resonance imaging in horses: Techniques, adverse events and opportunities

Use of contrast media in computed tomography and magnetic resonance imaging in horses: Techniques, adverse events and opportunities

Ultrasound‐guided injection of the cranial tibial artery for stem cell administration in horses

Principles of Therapy for Lameness

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