H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells

Kunitomi, Akira; Yuasa, Shinsuke; Sugiyama, Fumihiro; Saito, Yuki; Seki, Tomohisa; Kusumoto, Dai; Kashimura, Shin; Takei, Makoto; Tohyama, Shugo; Hashimoto, Hisayuki; Egashira, Toru; Tanimoto, Yoko; Mizuno, Saori; Tanaka, Sonosuke; Okuno, Hiroyuki; Yamazawa, Kazuki; Watanabe, Hideo; Oda, Mayumi; Kaneda, Ruri; Matsuzaki, Yasushi; Nagai, Toshihiro; Yagami, Ken Ichi; Tanaka, Mamoru; Fukuda, Keiichi

doi:10.1016/j.stemcr.2016.04.015

Cited by 44 publications

(26 citation statements)

References 41 publications

(50 reference statements)

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“…Could this be due to the lingering influence of maternal factors influencing survival at this embryonic developmental time‐point since zygotic genome activation occurs at the 8‐cell stage in humans? A role for the maternal histone 1 linker, H1foo, has been identified as normalizing the epigenome of cells during the process of pluripotency induction . H1foo is still present in the 8‐cell embryo while it is absent shortly after morula formation .…”

Section: Hesc Lines Established Directly As Naïvementioning

confidence: 99%

Concise Review: Lessons from Naïve Human Pluripotent Cells

Ware

2016

Stem Cells

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The naïve state of pluripotency is actively being explored by a number of labs. There is some controversy in the field as to the true identity of naïve human pluripotent cells as they are not exact mirrors of the mouse. The various reports published, though in basic agreement, present discrepancies in the characterization of the various lines, which likely reflect the etiology of these lines. The primary lesson learned from these contributions is that a human naïve state reflecting the pre-implantation human is likely to exist. The essential factors that will universally maintain the naïve state in human cells in vitro are not yet fully understood. These first need to be identified in order to describe the definitive characteristics of this state. Comparisons of naïve and primed human pluripotent cells have also highlighted consistencies between states and broadened our understanding of embryonic metabolism, epigenetic change required for development, embryonic DNA repair strategies and embryonic expression dynamics.

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Section: Hesc Lines Established Directly As Naïvementioning

confidence: 99%

Concise Review: Lessons from Naïve Human Pluripotent Cells

Ware

2016

Stem Cells

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“…In contrast, oocyte-based reprogramming is considered more efficient and synchronous. Recently, it has been shown that some oocyte-derived factors can indeed enhance the efficiency and quality of iPSC induction ( Gonzalez-Munoz et al., 2014 , Jiang et al., 2013 , Khaw et al., 2015 , Kunitomi et al., 2016 , Maekawa et al., 2011 , Shinagawa et al., 2014 ). We also found several highly expressed factors in oocytes in our previous study ( Wang et al., 2010 ), after which we aimed to illustrate their roles in somatic reprogramming.…”

Section: Resultsmentioning

confidence: 99%

“…The different efficiency between SCNT and iPSC technology ( Le et al., 2014 ) implies that some magical factors present in the oocyte might be able to promote iPSC induction. In fact, growing evidence suggests that some oocyte-specific factors can enhance the efficiency and quality of iPSC reprogramming ( Gaspar-Maia et al., 2013 , Huynh et al., 2016 , Jiang et al., 2013 , Khaw et al., 2015 , Kunitomi et al., 2016 , Maekawa et al., 2011 , Shinagawa et al., 2014 , Singhal et al., 2010 ). However, although many transcription factors have been shown to enhance the generation of iPSCs, the majority of oocyte factors remain poorly investigated.…”

Section: Introductionmentioning

confidence: 99%

Oocyte-Specific Homeobox 1, Obox1, Facilitates Reprogramming by Promoting Mesenchymal-to-Epithelial Transition and Mitigating Cell Hyperproliferation

Peng

et al. 2017

Stem Cell Reports

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SummaryMammalian oocytes possess fascinating unknown factors, which can reprogram terminally differentiated germ cells or somatic cells into totipotent embryos. Here, we demonstrate that oocyte-specific homeobox 1 (Obox1), an oocyte-specific factor, can markedly enhance the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts in a proliferation-independent manner and can replace Sox2 to achieve pluripotency. Overexpression of Obox1 can greatly promote mesenchymal-to-epithelial transition (MET) at early stage of OSKM-induced reprogramming, and meanwhile, the hyperproliferation of THY1-positive cells can be significantly mitigated. Subsequently, the proportion of THY1-negative cells and Oct4-GFP-positive cells increased dramatically. Further analysis of gene expression and targets of Obox1 during reprogramming indicates that the expression of Obox1 can promote epithelial gene expression and modulate cell-cycle-related gene expression. Taken together, we conclude that the oocyte-specific factor Obox1 serves as a strong activator for somatic cell reprogramming through promoting the MET and mitigating cell hyperproliferation.

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“…However, the nuclear level of H1foo decreased drastically at the two-cell stage [ 49 ], whereas the nuclear level of somatic H1 variants increased [ 48 ]. Interestingly, it has been suggested that the somatic H1 variants and H1foo are involved in the formation of condensed and relaxed chromatin, respectively [ 50 , 51 ].…”

Section: Epigenetic Factors In One- and Two-cell Embryosmentioning

confidence: 99%

Regulation of zygotic gene activation by chromatin structure and epigenetic factors

Funaya

Aoki

2017

Journal of Reproduction and Development

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After fertilization, the genomes derived from an oocyte and spermatozoon are in a transcriptionally silent state before becoming activated at a species-specific time. In mice, the initiation of transcription occurs at the mid-one-cell stage, which represents the start of the gene expression program. A recent RNA sequencing analysis revealed that the gene expression pattern of one-cell embryos is unique and changes dramatically at the two-cell stage. However, the mechanism regulating this alteration has not yet been elucidated. It has been shown that chromatin structure and epigenetic factors change dynamically between the one- and two-cell stages. In this article, we review the characteristics of transcription, chromatin structure, and epigenetic factors in one- and two-cell mouse embryos and discuss the involvement of chromatin structure and epigenetic factors in the alteration of transcription that occurs between these stages.

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H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells

Cited by 44 publications

References 41 publications

Concise Review: Lessons from Naïve Human Pluripotent Cells

Concise Review: Lessons from Naïve Human Pluripotent Cells

Oocyte-Specific Homeobox 1, Obox1, Facilitates Reprogramming by Promoting Mesenchymal-to-Epithelial Transition and Mitigating Cell Hyperproliferation

Regulation of zygotic gene activation by chromatin structure and epigenetic factors

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