Métodos de determinación de sensibilidad a los antimicrobianos en micobacterias

Alcaide, Fernando; Esteban, Jaime; González-Martín, Julià; Palacios, Juan-José

doi:10.1016/j.eimc.2016.04.008

Cited by 13 publications

(5 citation statements)

References 12 publications

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“…Inoculum of BCG was prepared from slant fresh growth Löwenstein-Jensen medium (15 days) and standardized according to the bacterial turbidity standard (McFarland standard) using a densitometer to a working dilution of 1 × 10 5 CFU/ml. 55,56 Antimicrobial effectiveness was determined as the lowest concentration of the drug that inhibits growth (less than 20 colonies).…”

Section: In Vitro Cell Assaymentioning

confidence: 99%

Structure‐based virtual screening and biological evaluation of novel inhibitors of mycobacterium Z‐ring formation

Rayevsky

Dariya

Liudmyla

et al. 2022

J of Cellular Biochemistry

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The major part of commercial prodrugs against Mycobacterium tuberculosis (Mtb) demonstrated a significant inhibitory effect on cell division and inhibition of bacterial growth in vitro. However, further implementation often failed to overcome the compensatory system of interchangeable cascades. This is the most common situation for the compounds, which hit the key enzymes activities involved in all basic stages of the cell cycle. We decided to find more compounds, which could affect a cytoskeleton complex playing important role in sensing the external signals, intracellular transport, and cell division. In general, the bacterial cytoskeleton is crucial for response to the environment and participates in cell-to-cell communication. In turn, filamentous temperature-sensitive Z (FtsZ) protein, a mycobacterial tubulin homolog, is essential for Z-ring formation and further bacteria cell division. We predicted the most preferable binding-sites and conducted a highthroughput virtual screening. Modeling results suggest that some compounds bind in a specific region on the surface Mtb FtsZ, which is absent in human, and other could hit GTPase activity of the FtsZ. Further in vitro studies confirmed that these novel molecules can efficiently bind to these pockets, demonstrating an effect on the polymerization state and kinetics mechanisms.The rescaling of the experiment on the cell line revealed that reported

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Section: In Vitro Cell Assaymentioning

confidence: 99%

Structure‐based virtual screening and biological evaluation of novel inhibitors of mycobacterium Z‐ring formation

Rayevsky

Dariya

Liudmyla

et al. 2022

J of Cellular Biochemistry

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“…The limitation of methods using solid medium is that they require several weeks of incubation due to M. tuberculosis's slow growth, while the newer methods in liquid medium (BACTEC MGIT 960, Becton-Dickinson Diagnostics, USA and VersaTREK, Thermo Scientifi c, USA) provide results more quickly (8-12 days), [10] but are very expensive because they are based on automated systems, limiting their adoption in many countries. [11,12] Understanding the molecular mechanisms of M. tuberculosis resistance has led to development of molecular tools, ranging from commercial systems (INNO-LiPA Rif.TB, INNO Genetics, Belgium, Genotype MTBDRplus and Genotype MTBDRsl, Hain Lifescience GmbH, Germany) to nucleic acid sequencing methods. Such methods are faster (not requiring bacterial culture, they can be done in a matter of hours) and highly sensitive, but their introduction in many countries is limited, since they require specifi c laboratory infrastructure and sophisticated equipment unavailable in countries with limited resources.…”

Section: Introductionmentioning

confidence: 99%

“…Such methods are faster (not requiring bacterial culture, they can be done in a matter of hours) and highly sensitive, but their introduction in many countries is limited, since they require specifi c laboratory infrastructure and sophisticated equipment unavailable in countries with limited resources. [11,12] Recently, WHO approved a group of alternative methods to investigate sensitivity to isoniazid and rifampicin; among these are the nitrate reductase assay (NRA), colorimetric assays using redox indicators, and the microscopic-observation drug-susceptibility assay. [13] For many years surveillance of antituberculosis drug resistance in Cuba was carried out using PM in Löwenstein-Jensen medium.…”

Section: Introductionmentioning

confidence: 99%

Antituberculosis Drug Resistance in Pulmonary Isolates of Mycobacterium tuberculosis, Cuba 2012–2014

2017

MEDICC Review

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INTRODUCTION Systematic surveillance of antituberculosis drug resistance allows identifi cation of multidrug-resistant and extensively drug-resistant isolates of Mycobacterium tuberculosis. Surveillance studies of antituberculosis drug resistance systematically conducted in Cuba for over 15 years have revealed low circulation of multidrugresistant tuberculosis, under 1% in new cases. OBJECTIVE Characterize antituberculosis drug resistance in isolates of M. tuberculosis recovered from patients with pulmonary tuberculosis in Cuba in 2012-2014. METHODSThe nitrate reductase assay was used to test 997 isolates of M. tuberculosis for sensitivity to isoniazid and rifampicin. Isolates identifi ed as multidrug resistant were tested for sensitivity to isoniazid, rifampicin, streptomycin, ethambutol, ofl oxacin, amikacin, kanamycin and capreomycin by the proportion method, as well as genetic resistance mutations in rpoB, katG, inhA, gyrA, rrs and embB genes, using GenoType MTBDRplus and MTBDRsl commercial kits. RESULTS Some 95.6% of isolates from new cases and 89.6% of isolates from previously treated patients were sensitive to isoniazid and rifampicin. Multidrug resistance was found in 0.8% of new and 5.2% of previously treated patients, a statistically signifi cant difference. One extensively drug-resistant isolate was detected among previously treated cases. All isolates examined with the molecular method had mutations in the rpoB gene, which is associated with resistance to rifampicin; only seven showed mutations in the katG gene and one in the inhA gene associated with isoniazid resistance. In one isolate, we found mutations in both gyrA and rrs genes, which are associated with resistance to fl uoroquinolones and second-line injectable drugs and therefore, extensive resistance.CONCLUSIONS Results corroborate the low frequency of multidrugresistant and extensively resistant M. tuberculosis strains in Cuba and highlight the need for continuous improvement of surveillance of antituberculosis drug resistance in Cuba.

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“…Both in Spain and Latin America, the prevalence of patients with musculoskeletal pain who have appealed to medical care are often attended by a general doctor, who in most of cases, prescribe NSAIDs (Nonsteroidal antiinflammatory drugs). Therefore, several diseases that should be managed by the rheumatologist there are not diagnosed in time [45].…”

Section: Present Problematic and Hypothesismentioning

confidence: 99%

“…The viscosity values have been obtained using the equation 45 Table 17.Viscosity results for glycerol aqueous dilutions Figure 65. ΔfS and ΔΓleft behavior for glycerol dilution samples.…”

Section: Sensibility and Limit Of Detection Of The Biosensormentioning

confidence: 99%

Diseño y evaluación de un biosensor basado en resonadores de cristal de cuarzo (QCR) para caracterizar muestras biológicas relacionadas con enfermedades artríticas

Ahumada¹,

Armando²

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IVResumen de la tesis de Luis Armando Carvajal Ahumada, presentada como requisito parcial para la obtención del grado de DOCTOR en INGENIERÍA BIOMÉDICA. Madrid, 03 de Abril de 2017 DISEÑO Y EVALUACIÓN DE UN SENSOR BASADO EN RESONADORES DE CRISTAL DE CUARZO (QCR) PARA CARACTERIZAR MUESTRAS BIOLÓGICAS RELACIONADAS CON LA DIAGNOSIS DE ENFERMEDADES ARTRÍTICASResumen:Los resonadores de cristal de cuarzo son dispositivos ampliamente utilizados como biosensores debido a su alta sensibilidad ante fenómenos físico-químicos que ocurren en su superficie, a la reproducibilidad de las mediciones y por ser transductores fáciles de utilizar. Sauerbrey[1] fue el primero en demostrar que el resonador cristal de cuarzo puede ser utilizado como una microbalanza útil para caracterizar pequeñas deposiciones de masa en el electrodo del cristal. Sauerbrey demostró que, si se deposita una delgada capa de material rígido sobre el cristal de cuarzo, tanto el cristal como el material depositado vibrarán a la misma frecuencia y se generará un cambio en la frecuencia de resonancia inicial del cristal. Específicamente, la frecuencia de resonancia disminuye al aumentar la cantidad de masa depositada.El cristal de cuarzo también puede operar en medio fluidos. En este caso además del desplazamiento en frecuencia del cristal, se genera una pérdida significativa en el factor de calidad del resonador. Para fluidos Newtonianos (viscosidad constante), Kanazawa y Gordon[2] demostraron que el desplazamiento en frecuencia que sufre el resonador así como el cambio en el ancho de la curva de admitancia, depende de la densidad y la viscosidad del líquido. Este fenómeno permite utilizar los resonadores de cristal de cuarzo como pequeños viscosímetros siempre que se conozca la densidad del fluido a analizar.En el caso de deposición de fluidos viscoelásticos, la medición del cambio en la frecuencia de resonancia del cristal no es suficiente para obtener el valor de viscosidad del fluido. Por tanto, se requiere de la medición de una segunda variable (mitad del ancho de media banda -Γ) para obtener el valor correcto de la viscosidad del fluido depositado.[3] Para fluidos pseudoplásticos (viscosidad aparente dependiente de la velocidad de cizallamiento aplicada), se requiere de un modelo matemático que explique el comportamiento de la viscosidad del fluido a distintas velocidades de cizallamiento. En particular para el análisis de muestras con este comportamiento, se ha elegido el modelo Rouse ya que explica con suficiente detalle el comportamiento pseudoplástico y resulta simple de aplicar a las medidas experimentales.En este trabajo se propone el diseño de un biosensor basado en un resonador de cristal de cuarzo (QCR) para la caracterización de muestras biológicas que emulan el comportamiento del líquido sinovial.El líquido sinovial es una mezcla de plasma dializado y ácido hialurónico con comportamiento pseudoplástico. Su principal función es la de actuar como lubricante en las articulaciones V manteniendo al mínimo la fricción entre los huesos, especialment...

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Métodos de determinación de sensibilidad a los antimicrobianos en micobacterias

Cited by 13 publications

References 12 publications

Structure‐based virtual screening and biological evaluation of novel inhibitors of mycobacterium Z‐ring formation

Structure‐based virtual screening and biological evaluation of novel inhibitors of mycobacterium Z‐ring formation

Antituberculosis Drug Resistance in Pulmonary Isolates of Mycobacterium tuberculosis, Cuba 2012–2014

Diseño y evaluación de un biosensor basado en resonadores de cristal de cuarzo (QCR) para caracterizar muestras biológicas relacionadas con enfermedades artríticas

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