Analysis of the Mild strain of tomato yellow leaf curl virus, which overcomes Ty-2 gene–mediated resistance in tomato line H24

Ohnishi, Jun; Yamaguchi, Haruhisa; Saito, Atsushi

doi:10.1007/s00705-016-2898-4

Cited by 37 publications

(32 citation statements)

References 40 publications

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“…Of these transmissible viruses, Tomato yellow leaf curl virus (TYLCV; Begomovirus ) is the most devastating causal virus on tomato crops in many tropical, subtropical and temperate regions worldwide (Moriones and Navas-Castillo 2000). In Japan, TYLCV spread along with whitefly and indicated its dispersion throughout 38 prefectures by 2014 since the occurrence of TYLCV on tomato was first reported in Japan (Kato et al 1998, Matsuura and Hoshino 2008, Ohnishi et al 2016). However, there is no report of TYLCV incidence on pepino plants to date.…”

Section: Discussionmentioning

confidence: 99%

Incidence of pests and viral disease on pepino (Solanum muricatum Ait.) in Kanagawa Prefecture, Japan

Kim¹,

Ishikawa²,

Yamada³

et al. 2017

BDJ

905

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BackgroundThe solanaceous fruit crop pepino (Solanum muricatum Ait.), originating in the Andes, is grown commercially in South American countries and New Zealand. In these areas, pests and diseases of pepino have been identified well; however, to date, these have seldom been investigated in detail in Japan. Herein, we attempt to reconstruct an agricultural production system for commercial pepino crops in Japan, and evaluate the incidence of pests and viral diseases on pepino. The findings of this study will facilitate in developing a better crop system for the commercial cultivation of healthy pepino fruits.New informationA total of 11 species, comprising nine insects and two mites, were recognized as pests of pepino plants in our experimental fields in Kanagawa Prefecture, central Honshu, Japan. Of these pest species, the two-spotted spider mite Tetranychus urticae Koch, 1836 and the cotton aphid Aphis gossypii Glover, 1877, were remarkably abundant than the other pest species. Eventually, 13 species, including two previously recorded, are currently recognized as the pests of pepino in Japan. With regard to viruses, we tested two species Alfalfa mosaic virus (AMV) and Cucumber mosaic virus (CMV), as well as three genera Carlavirus, Potexvirus, and Potyvirus. No virus was detected in symptomatic pepino leaves collected in our experimental fields. This is a first report on the identification of pests on pepino plants in Kanagawa Prefecture, Japan and elucidates the relationship between currently occurring pests of pepino plants and potential viral pathogens that they can transmit.

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Section: Discussionmentioning

confidence: 99%

Incidence of pests and viral disease on pepino (Solanum muricatum Ait.) in Kanagawa Prefecture, Japan

Kim¹,

Ishikawa²,

Yamada³

et al. 2017

BDJ

905

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“…Previously, breeding efforts contributed to the adoption of Ty‐2 ‐based cultivars/hybrids in India. However, in recent years, it has been observed that such cultivars/hybrids are susceptible to begomoviruses (Barbieri et al ., 2010; Ohnishi et al ., 2016; Prabhandakavi et al ., 2018). It was therefore necessary to reexamine the effectiveness of various Ty resistance genes against ToLCBaV infection.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Ohnishi et al . (2016) demonstrated that the overlapping C4/Rep region of the genome of the milder strain of TYLCV was associated with the ability of this strain to overcome Ty‐2 ‐mediated resistance in the tomato line H24. Prasanna et al .…”

Section: Introductionmentioning

confidence: 99%

Assessment of the effectiveness of Ty genes in tomato against tomato leaf curl Bangalore virus

et al. 2020

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Tomato leaf curl Bangalore virus (ToLCBaV), a monopartite begomovirus transmitted by the whitefly Bemisia tabaci, has become a major constraint in tomato production in the Indian subcontinent. Earlier breeding efforts in India led to the adoption of tomato cultivars carrying the resistance gene Ty‐2. However, it has been observed recently that such cultivars/hybrids are susceptible to begomoviruses. This requires the identification of additional or new sources of resistance against ToLCBaV. The present study was undertaken to assess the effectiveness of several Ty genes in providing resistance against infection by ToLCBaV. The kinetics of virus multiplication in different Ty resistance gene stocks of tomato were estimated and compared using quantitative PCR data. Accumulation of viral genomic units and symptom severity were lower in tomato lines carrying Ty‐3 and Ty‐2 + Ty‐3 compared with those carrying Ty‐2 alone. All tested lines carrying Ty‐2 showed typical tomato leaf curl disease symptoms. Tomato lines carrying Ty‐2 + ty‐5 and ty‐5 + Ty‐6 combinations had a significantly higher disease severity index and viral genomic units compared to those carrying Ty‐3 and Ty‐2 + Ty‐3 at 30 days postinoculation. The accumulation level of ToLCBaV genomic units serves as a good indicator for resistance selection along with other parameters of disease. We present here a comprehensive overview of the effectiveness of Ty‐2, Ty‐3, and ty‐5 alone as well as the combinations Ty‐2 + Ty‐3 and Ty‐2 + ty‐5 against ToLCBaV.

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“…For example the TYLCV-IS76 strain, a recombinant between TYLCV-IL and the Spanish strain of TYLC-Sardinia virus (TYLCSV-ES), which can accumulate better than its parental strains in cultivars carrying the Ty-1 gene ( Belabess et al, 2015 , 2016 ). It has been shown that Ty-2 based resistance can be overcome by TYLCSV and the TYLCV-Mld strain which has a TYLCSV-like C4 protein ( Barbieri et al, 2010 ; Tomás et al, 2011 ; Ohnishi et al, 2016 ). Therefore, the TYLCV symptomless accessions identified in this study represent a treasure of resources to tomato breeders.…”

Section: Discussionmentioning

confidence: 99%

“…Up till now, introgressions of Ty-1 , Ty-2 , and Ty-3 into cultivated tomato have been the major focus in breeding programs. Ty-2 based resistance can be overcome by TYLCV-related Tomato yellow leaf curl Sardinia virus (TYLCSV) ( Barbieri et al, 2010 ) and breakdown of Ty-2 mediated resistance has been demonstrated recently by an isolate of the Mild strain of TYLCV (TYLCV-Mld) ( Ohnishi et al, 2016 ). Ty-1 -mediated resistance is not suitable to use under high disease pressure which leads to resistance breakage in some cases ( García-Cano et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm

et al. 2018

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Tomato yellow leaf curl virus (TYLCV) is a virus species causing epidemics in tomato (Solanum lycopersicum) worldwide. Many efforts have been focused on identification of resistance sources by screening wild tomato species. In many cases, the accession numbers were either not provided in publications or not provided in a consistent manner, which led to redundant screenings. In the current study, we summarized efforts on the screenings of wild tomato species for TYLCV resistance from various publications. In addition, we screened 708 accessions from 13 wild tomato species using different inoculation assays (i.e., whitefly natural infection and Agrobacterium-mediated inoculation) from which 138 accessions exhibited no tomato yellow leaf curl disease (TYLCD) symptoms. These symptomless accessions include 14 accessions from S. arcanum, 43 from S. chilense, 1 from S. chmielewskii, 28 from S. corneliomulleri, 5 from S. habrochaites, 4 from S. huaylasense, 2 from S. neorickii, 1 from S. pennellii, 39 from S. peruvianum, and 1 from S. pimpinellifolium. Most of the screened S. chilense accessions remained symptomless. Many symptomless accessions were also identified in S. arcanum, S. corneliomulleri, and S. peruvianum. A large number of S. pimpinellifolium accessions were screened. However, almost all of the tested accessions showed TYLCD symptoms. Further, we studied allelic variation of the Ty-1/Ty-3 gene in few S. chilense accessions by applying virus-induced gene silencing and allele mining, leading to identification of a number of allele-specific polymorphisms. Taken together, we present a comprehensive overview on TYLCV resistance and susceptibility in wild tomato germplasm, and demonstrate how to study allelic variants of the cloned Ty-genes in TYLCV-resistant accessions.

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Analysis of the Mild strain of tomato yellow leaf curl virus, which overcomes Ty-2 gene–mediated resistance in tomato line H24

Cited by 37 publications

References 40 publications

Incidence of pests and viral disease on pepino (Solanum muricatum Ait.) in Kanagawa Prefecture, Japan

Incidence of pests and viral disease on pepino (Solanum muricatum Ait.) in Kanagawa Prefecture, Japan

Assessment of the effectiveness of Ty genes in tomato against tomato leaf curl Bangalore virus

Resistance to Tomato Yellow Leaf Curl Virus in Tomato Germplasm

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