Impact of homeopathic remedies on the expression of lineage differentiation genes: an in vitro approach using embryonic stem cells

Jyoti, Saras; Tandon, Simran

doi:10.1016/j.homp.2015.11.002

Cited by 4 publications

(5 citation statements)

References 26 publications

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“…The Indian group used a range of methods including PCR but with the objective of demonstrating safety in pregnancy. 2 They found no significant difference in gene expression between Nux vomica 30c and Sepia officinalis 30c compared to solvent in mouse embryonic stem cells. They conclude that this supports their safety in pregnancy.…”

Section: Pcrmentioning

confidence: 93%

PCR, pre-sensitisation and polycrystals

Fisher¹

2016

Homeopathy

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Section: Pcrmentioning

confidence: 93%

PCR, pre-sensitisation and polycrystals

Fisher¹

2016

Homeopathy

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“…They found that Sepia, and also Nux vomica, led to modulations in the expression of certain lineage-specific genes, but these differences were not significant with respect to their solvent controls. 75 Several randomized controlled trials, clinical observational studies and case reports have indicated the most important HMs that seem to improve menopausal complaints. The HMM fully describes their most characteristic symptoms.…”

Section: Role Of Dopamine In the Pathophysiology Of Depressionmentioning

confidence: 99%

“…She becomes depressed and indifferent toward anything; only wants to be alone, losing enthusiasm toward life; is irritable and easily offended; expresses great sadness with much weeping, especially when telling her symptoms; feels sluggish and dull, both physically and mentally; has lack of vital heat during chronic diseases; experiences hot flashes, with weakness and perspiration, during menopause; has sensitivity to cold air; faints easily from extremes of temperature or getting wet; and has a bearing-down sensation and constipation. [75][76][77][78] Besides the mental symptoms previously described, it is noticeable that the HMM also describes skin features in Sepia officinalis such as yellowness of face and conjunctiva, yellow spots on the chest, brown saddle over upper parts of cheeks and nose, herpetic eruptions, and lentigo. 63 The Sepia officinalis ink gland is involved in the unique defense mechanism of this cephalopod.…”

Section: Sepia Officinalismentioning

confidence: 99%

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Understanding Why Homeopathic Medicines are Used for Menopause: Searching for Insights into Neuroendocrine Features

Macías-Cortés

2023

Homeopathy

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Background Menopause is a physiological event that marks the end of a woman's reproductive stage in life. Vasomotor symptoms and changes in mood are among its most important effects. Homeopathy has been used for many years in treating menopausal complaints, though clinical and pre-clinical research in this field is limited. Homeopathy often bases its prescription on neuropsychiatric symptoms, but it is unknown if homeopathic medicines (HMs) exert a neuroendocrine effect that causes an improvement in vasomotor symptoms and mood during menopause. Objectives The study's objectives were to address the pathophysiological changes of menopause that could help in the understanding of the possible effect of HMs at a neuroendocrine level, to review the current evidence for two of the most frequently prescribed HMs for menopause (Lachesis mutus and Sepia officinalis), and to discuss the future directions of research in this field. Methods An extensive literature search for the pathophysiologic events of menopause and depression, as well as for the current evidence for HMs in menopause and depression, was performed. Results Neuroendocrine changes are involved in the pathophysiology of vasomotor symptoms and changes in mood during menopause. Gonadal hormones modulate neurotransmitter systems. Both play a role in mood disorders and temperature regulation. It has been demonstrated that Gelsemium sempervirens, Ignatia amara and Chamomilla matricaria exert anxiolytic effects in rodent models. Lachesis mutus and Sepia officinalis are frequently prescribed for important neuropsychiatric and vasomotor symptoms. Dopamine, a neurotransmitter involved in mood, is among the constituents of the ink of the common cuttlefish, Sepia officinalis. Conclusion Based on all the pathophysiologic events of menopause and the improvement in menopausal complaints that certain HMs show in daily practice, these medicines might have a direct or indirect neuroendocrine effect in the body, possibly triggered via an as-yet unidentified biological mechanism. Many unanswered questions in this field require further pre-clinical and clinical research.

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“…17 The working concentration for each potency of the drug was 5 μL/mL for all the in vitro studies and was based on our previous published reports. 18,19 Cisplatin was used as positive control at the specified concentration mentioned for each cell line: 1.5 μM (MCF 7) and 1.83 μM (MDA-MB-468). The concentrations of cisplatin used in our study for the specific cell type were based on crystal violet viability assay.…”

Section: Homeopathic Medicinesmentioning

confidence: 99%

In Vitro Assessment of Homeopathic Potencies of Hydrastis canadensis on Hormone-Dependent and Independent Breast Cancer

et al. 2020

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Abstract Background Breast cancer is the second leading cause of cancer-related deaths in women. Conventional treatment such as chemotherapy, hormonal therapy and radiotherapy has decreased the mortality rate among cancer patients but has also revealed long-term side effects. Drug resistance and toxicity to normal cells compound the problems associated with the use of modern medicines. Hence, complementary or alternative treatment options are being explored. The current study, using different homeopathic potencies of Hydrastis canadensis, was conducted to distinguish between any effects they might have on hormone-dependent and independent breast cancer. Materials and Methods The cytotoxic effect of homeopathic medicine Hydrastis on hormone-dependent (MCF 7) and hormone-independent (MDA-MB-468) breast cancer cells was assessed using viability and colony-forming assays after 48 or 72 hours of treatment. Flow cytometry-based Annexin V-PI (propidium iodide), caspase 3 and cell cycle analysis was performed following treatment of cells with mother tincture or various potencies of Hydrastis (1C, 2C, 30C, 200C). Results Different potencies of Hydrastis displayed selective cytotoxic effects against MCF 7 cells, but only marginal effects against MDA-MB-468. The maximum cytotoxicity was established in the case of 1C following 72 hours of treatment. Treatment of breast cancer cells revealed an increase in the G0/G1 cell population, along with an increase in the caspase 3 levels and induction of apoptosis. Conclusion Hydrastis may have a selective cytotoxic effect against hormone-dependent breast cancer MCF 7 cells, leading to cell cycle arrest in the G0/G1 phase, which could be the plausible reason for the induction of apoptosis. The results need to be validated in vivo.

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Impact of homeopathic remedies on the expression of lineage differentiation genes: an in vitro approach using embryonic stem cells

Abstract: Our study for the first time has shown the feasibility of using ES cells in the developmental toxicity testing of remedies. The results suggest that they are not associated with developmental toxicity.

Cited by 4 publications

References 26 publications

PCR, pre-sensitisation and polycrystals

PCR, pre-sensitisation and polycrystals

Understanding Why Homeopathic Medicines are Used for Menopause: Searching for Insights into Neuroendocrine Features

In Vitro Assessment of Homeopathic Potencies of Hydrastis canadensis on Hormone-Dependent and Independent Breast Cancer

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