Finding FISH in a small pond

Abstract

Advanced microscopy and labeling techniques reveal that bacteria localize mRNAs within their cells in a similar way to eukaryotes.

Sub-cellular mRNA localization modulates the regulation of gene expression by small RNAs in bacteria

“…In particular, it has been hypothesized that the threshold-linear response, by which sRNA transcription sets a threshold for target expression, could be important for bacteria to fight random fluctuations and transient signals [12]. In recent years, these features and others have been described in detail, both theoretically and experimentally [17][18][19][20].Recent advances in super-resolution microscopy and fluorescence in situ hybridization (FISH) allow for direct measurement of the spatial arrangement of macromolecules in vivo [16,[21][22][23][24][25][26][27]. As a result of such efforts it has been experimentally shown that the mRNAs of some genes can be localized to specific regions in the cell [28][29][30][31].…”

“…Recent advances in super-resolution microscopy and fluorescence in situ hybridization (FISH) allow for direct measurement of the spatial arrangement of macromolecules in vivo [16,[21][22][23][24][25][26][27]. As a result of such efforts it has been experimentally shown that the mRNAs of some genes can be localized to specific regions in the cell [28][29][30][31].…”