Involvement of CmWRKY10 in Drought Tolerance of Chrysanthemum through the ABA-Signaling Pathway

Jaffar, Muhammad Abuzar; Song, Aiping; Faheem, Muhammad; Chen, Sumei; Jiang, Jiafu; Liu, Chen; Fan, Qing; Chen, Fadi

doi:10.3390/ijms17050693

Cited by 59 publications

(34 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The p35S::GFP- CmMYB19 construct and the empty pMDC43 vector were introduced into onion epidermal cells via particle bombardment (PDS-1000; Bio-Rad, Tokyo, Japan). Transformed cells were held for 16 h on Murashige and Skoog (MS) medium in the dark, and then subjected to confocal laser microscopy in order to monitor GFP activity [40]. …”

Section: Methodsmentioning

confidence: 99%

CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis

Wang

Sheng

Zhang

et al. 2017

IJMS

Self Cite

View full text Add to dashboard Cite

Abstract:The gene encoding the MYB (v-myb avian myeloblastosis vira l oncogene homolog) transcription factor CmMYB19 was isolated from chrysanthemum. It encodes a 200 amino acid protein and belongs to the R2R3-MYB subfamily. CmMYB19 was not transcriptionally activated in yeast, while a transient expression experiment conducted in onion epidermal cells suggested that the CmMYB19 product localized to the nucleus. CmMYB19 transcription was induced by aphid (Macrosiphoniella sanborni) infestation, and the abundance of transcript was higher in the leaf and stem than in the root. The over-expression of CmMYB19 restricted the multiplication of the aphids. A comparison of transcript abundance of the major genes involved in lignin synthesis showed that CmPAL1 (phenylalanine ammonia lyase 1), CmC4H (cinnamate4 hydroxylase), Cm4CL1 (4-hydroxy cinnamoyl CoA ligase 1), CmHCT (hydroxycinnamoyl CoA-shikimate/quinate hydroxycinnamoyl transferase), CmC3H1 (coumarate3 hydroxylase1), CmCCoAOMT1 (caffeoyl CoA O-methyltransferase 1) and CmCCR1 (cinnamyl CoA reductase1) were all upregulated, in agreement with an increase in lignin content in CmMYB19 over-expressing plants. Collectively, the over-expression of CmMYB19 restricted the multiplication of the aphids on the host, mediated by an enhanced accumulation of lignin.

show abstract

Section: Methodsmentioning

confidence: 99%

CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis

Wang

Sheng

Zhang

et al. 2017

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Heterologous expression of CmMYB2 enhanced drought tolerance in Arabidopsis thaliana by increasing plant sensitivity to ABA and reducing stomata aperture 17 . Moreover, recent studies have indicated that the CmWRKY10, DgNAC1, and ClCBF1 transcription factors could also improve the level of drought tolerance in C. morifolium [18][19][20] . In addition to the study of gene function, high-throughput sequencing was applied to identify candidate genes in response to drought stress.…”

mentioning

confidence: 99%

Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis

Yang

Guo

Zhong

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Opisthopappus taihangensis (Ling) Shih, as a relative of chrysanthemum, mainly survives on the cracks of steep slopes and cliffs. Due to the harsh environment in which O. taihangensis lives, it has evolved strong adaptive traits to drought stress. The root system first perceives soil water deficiency, triggering a multi-pronged response mechanism to maintain water potential; however, the drought tolerance mechanism of O. taihangensis roots remains unclear. Therefore, roots were selected as materials to explore the physiological and molecular responsive mechanisms. We found that the roots had a stronger water retention capacity than the leaves. This result was attributed to ABA accumulation, which promoted an increased accumulation of proline and trehalose to maintain cell osmotic pressure, activated SOD and POD to scavenge ROS to protect root cell membrane structure and induced suberin depositions to minimize water backflow to dry soil. Transcriptome sequencing analyses further confirmed that O. taihangensis strongly activated genes involved in the ABA signalling pathway, osmolyte metabolism, antioxidant enzyme activity and biosynthesis of suberin monomer. overall, these results not only will provide new insights into the drought response mechanisms of O. taihangensis but also will be helpful for future drought breeding programmes of chrysanthemum. Drought stress is one of the most common abiotic stresses that threatens the healthy growth and development of plants. With the further aggravation of global warming and shortages of fresh water associated with population growth, it is estimated that drought stress will severely reduce the yield and quality of crops and ornamental plants 1. Therefore, further exploration of the physiological and molecular mechanisms is necessary for breeding drought-tolerant plants. Plants have evolved physiological, biochemical and molecular strategies to adapt to arid environments and to prevent cells from water deficiency. Physiological adaptability, including abscisic acid (ABA) content changes, proline accumulation, and superoxide dismutase (SOD) and peroxidase (POD) enzyme activities, are fundamental for plants to withstand drought stress 2-5. Drought-responsive molecular mechanisms have been divided into two terms: those that directly protect plants against drought stress and those that regulate targeted gene expression and signal transduction in plants in response to drought 6. The first term includes genes encoding proteins that function by protecting cell turgor, such as enzymes participating in the biosynthesis of various osmoprotectants 4,7,8. Moreover, late-embryogenesis-abundant proteins, chaperones and antioxidant enzymes directly prevent plants from drought damage. The second term of genes mainly comprises transcription factors, which are activated by signal transduction pathways and regulate functional genes 2,9. Furthermore, protein kinases, protein phosphatases, enzymes related to phospholipid metabolism and ubiquitin ligase play significant roles in the signal trans...

show abstract

“…After two days of drought and salt treatment, the activity levels of SOD, POD and CAT in the leaves were determined by the method described previously [32]. For the determination of Pro (proline) and MDA (malondialdehyde) content, the transgenic lines and the WT were selected one week after exposure to drought/salt stress, as described previously [50].…”

Section: Measurement Of Antioxidant Enzymes Proline and Malondialdehydementioning

confidence: 99%

Tartary Buckwheat Transcription Factor FtbZIP5, Regulated by FtSnRK2.6, Can Improve Salt/Drought Resistance in Transgenic Arabidopsis

Zhao

Wang

et al. 2020

IJMS

View full text Add to dashboard Cite

bZIP transcription factors have been reported to be involved in many different biological processes in plants. The ABA (abscisic acid)-dependent AREB/ABF-SnRK2 pathway has been shown to play a key role in the response to osmotic stress in model plants. In this study, a novel bZIP gene, FtbZIP5, was isolated from tartary buckwheat, and its role in the response to drought and salt stress was characterized by transgenic Arabidopsis. We found that FtbZIP5 has transcriptional activation activity, which is located in the nucleus and specifically binds to ABRE elements. It can be induced by exposure to PEG6000, salt and ABA in tartary buckwheat. The ectopic expression of FtbZIP5 reduced the sensitivity of transgenic plants to drought and high salt levels and reduced the oxidative damage in plants by regulating the antioxidant system at a physiological level. In addition, we found that, under drought and salt stress, the expression levels of several ABA-dependent stress response genes (RD29A, RD29B, RAB18, RD26, RD20 and COR15) in the transgenic plants increased significantly compared with their expression levels in the wild type plants. Ectopic expression of FtbZIP5 in Arabidopsis can partially complement the function of the ABA-insensitive mutant abi5-1 (abscisic acid-insensitive 5-1). Moreover, we screened FtSnRK2.6, which might phosphorylate FtbZIP5, in a yeast two-hybrid experiment. Taken together, these results suggest that FtbZIP5, as a positive regulator, mediates plant tolerance to salt and drought through ABA-dependent signaling pathways.

show abstract

Involvement of CmWRKY10 in Drought Tolerance of Chrysanthemum through the ABA-Signaling Pathway

Cited by 59 publications

References 49 publications

CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis

CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis

Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis

Tartary Buckwheat Transcription Factor FtbZIP5, Regulated by FtSnRK2.6, Can Improve Salt/Drought Resistance in Transgenic Arabidopsis

Contact Info

Product

Resources

About