Evaluation of immunohistochemical staining for glucagon in human pancreatic tissue

Gurlo, Tatyana; Butler, Peter C.; Butler, Alexandra E.

doi:10.1179/2046023615y.0000000013

Cited by 3 publications

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References 11 publications

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“…Immunohistochemical evaluations are therefore indispensable diagnostic tools of anatomical histopathology that gives information on β‐ and α‐cell mass, endocrine cell distribution within the pancreas, islet β‐cell composition and islet morphology in diabetic rats. [ 48 ] In this study, we observed immunohistochemically that streptozotocin significantly reduced endocrine cell distribution and islet β‐cell composition in negative control rats evident by low insulin immunoreactivity in this group. This is in consonance with several reports where STZ was implicated in the induction of these deranged features.…”

Section: Discussionmentioning

confidence: 61%

Metabolome modulatory effects of Kigelia africana (Lam.) Benth. fruit extracts on oxidative stress, hyperlipidaemic biomarkers in STZ-induced diabetic rats and antidiabetic effects in 3T3 L1 adipocytes

Fagbohun

Olawoye

Ademakinwa

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives The management of diabetes is considered a global problem, and a cure is yet to be discovered. This study investigated the modulatory effect of Kigelia africana fruit on oxidative stress and hyperlipidaemic biomarkers in STZinduced diabetic rats, profiled phytoconstituents using GC-TOF-MS and evaluated antidiabetic effects on 3T3 L1 adipocytes. Methods Thirty male Wistar rats (120-150 g) were divided into six groups (n = 5). Diabetes was induced by a single intraperitoneal injection of STZ (60 mg/kg) and treated with 100, 200 and 400 of hexane fraction of KA for 28 days. Immunohistochemical evaluation was carried out using avidin-biotin immunoperoxidase (ABI) method. Catalase and SOD activities as well as the levels of total protein, albumin, bilirubin, triglyceride, cholesterol, and high-density lipoprotein were measured. Key findings The expressions of oxidative stress and hyperlipidaemic biomarkers alongside fasting blood glucose concentrations were remarkedly decreased in KAtreated diabetic rats. Moreover, there was a significant increase in endocrine cell distribution, area covered with increase in b-cell mass, composition and morphology of KA-treated animals. Additionally, there was constant up-regulation in 3T3 L1 adipocytes due to the presence of phytoconstituents. Conclusion Kigelia africana fruit can act as a modulatory agent due to its ameliorative effects against oxidative stress. Highlights • Kigelia africana fruit increases b-cell mass within the pancreas of diabetic rats • K. africana fruit consists of 376 metabolites and chemical compounds • K. africana fruit exhibited an up-regulation glucose uptake in 3T3 L1 adipocytes • K. africana fruit can maintain or reverse biochemical integrity of diabetic rats Oladapo F. Fagbohun et al. Modulatory effects of K. africana fruits

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Section: Discussionmentioning

confidence: 61%

Metabolome modulatory effects of Kigelia africana (Lam.) Benth. fruit extracts on oxidative stress, hyperlipidaemic biomarkers in STZ-induced diabetic rats and antidiabetic effects in 3T3 L1 adipocytes

Fagbohun

Olawoye

Ademakinwa

et al. 2020

Journal of Pharmacy and Pharmacology

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Nongenomic action of aldosterone on colocalization of angiotensin II type 1 and type 2 receptors in rat kidney

Sinphitukkul

Manotham

Eiam‐Ong

2018

Journal of Histotechnology

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Pancreatic alpha-cell mass across adult human lifespan

Moin

Cory

Gurlo

et al. 2020

European Journal of Endocrinology

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Aim To establish pancreatic alpha-cell mass in lean, non-diabetic humans over the adult lifespan, performed as a follow-up study to beta-cell mass across the adult human lifespan. Methods We examined human pancreatic autopsy tissue from 66 lean, non-diabetic individuals aged from 30 to 102 years, grouped into deciles: 3rd (30–39 years), 4th (40–49 years), 5th (50–59 years), 6th (60–69 years), 7th (70–79 years), 8th (80–89 years) and 9th deciles (90+ years). Sections of pancreas were immunostained for glucagon and analyzed for fractional alpha-cell area. Population-based pancreatic volume data were used to calculate alpha-cell mass. Results With advanced age, the exocrine pancreas undergoes atrophy demonstrated by increased fat area (as % exocrine area) (0.05 ± 0.01 vs 1.6 ± 0.7% fat area of total exocrine pancreas, 3rd vs 9th decile, P < 0.05). Consequently, islet density increases with age (2.7 ± 0.4 vs 10.5 ± 3.3 islets/mm2, 3rd vs 9th decile, P < 0.05). Alpha-cell fractional area increases with advanced age (0.34 ± 0.05% vs 0.73 ± 0.26%, 3rd vs 9th decile, P < 0.05). However, alpha-cell mass remains constant at ~190 mg throughout the adult lifespan in lean, non-diabetic humans. Within islets, alpha-cell distribution between mantle and core is unchanged across deciles (1862 ± 220 vs 1945 ± 200 vs 1948 ± 139 alpha cells in islet mantle/mm2, 3rd vs 6th vs 9th decile, P = 0.93 and 1912 ± 442 vs 1449 ± 123 vs 1514 ± 168 alpha cells in islet core/mm2, 3rd vs 6th vs 9th decile, P = 0.47), suggesting that human islets retain their structural organization in the setting of age-related exocrine atrophy. Conclusions Consistent with our previous findings for beta-cell mass, alpha-cell mass remains constant in humans, even with advanced age. Pancreatic endocrine cells are much more robustly preserved than exocrine cells in aged humans, and islets maintain their structural integrity throughout life.

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Evaluation of immunohistochemical staining for glucagon in human pancreatic tissue

Cited by 3 publications

References 11 publications

Metabolome modulatory effects of Kigelia africana (Lam.) Benth. fruit extracts on oxidative stress, hyperlipidaemic biomarkers in STZ-induced diabetic rats and antidiabetic effects in 3T3 L1 adipocytes

Metabolome modulatory effects of Kigelia africana (Lam.) Benth. fruit extracts on oxidative stress, hyperlipidaemic biomarkers in STZ-induced diabetic rats and antidiabetic effects in 3T3 L1 adipocytes

Nongenomic action of aldosterone on colocalization of angiotensin II type 1 and type 2 receptors in rat kidney

Pancreatic alpha-cell mass across adult human lifespan

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