Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Soteriou, Despina; Kostic, Lana; Sedov, Egor; Yosefzon, Yahav; Steller, Hermann; Fuchs, Yaron

doi:10.3791/53931

Cited by 14 publications

(13 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dorsal skin from 8-week-old mouse was shaved and harvested. Underlying adipose tissue was removed before incubting in trypsin overnight at 4 °C or 2 h at 37 °C 42 . Epidermis and hairs were collected and filtered before sorting 43 .…”

Section: Methodsmentioning

confidence: 99%

Blimp1+ cells generate functional mouse sebaceous gland organoids in vitro

et al. 2019

Self Cite

View full text Add to dashboard Cite

Most studies on the skin focus primarily on the hair follicle and interfollicular epidermis, whereas little is known regarding the homeostasis of the sebaceous gland (SG). The SG has been proposed to be replenished by different pools of hair follicle stem cells and cells that resides in the SG base, marked by Blimp1. Here, we demonstrate that single Blimp1 + cells isolated from mice have the potential to generate SG organoids in vitro. Mimicking SG homeostasis, the outer layer of these organoids is composed of proliferating cells that migrate inward, undergo terminal differentiation and generating lipid-filled sebocytes. Performing confocal microscopy and mass-spectrometry, we report that these organoids exhibit known markers and a lipidomic profile similar to SGs in vivo. Furthermore, we identify a role for c-Myc in sebocyte proliferation and differentiation, and determine that SG organoids can serve as a platform for studying initial stages of acne vulgaris, making this a useful platform to identify potential therapeutic targets.

show abstract

Section: Methodsmentioning

confidence: 99%

Blimp1+ cells generate functional mouse sebaceous gland organoids in vitro

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The HF stem cells are located in the bulge region within the ORS [35]. During homeostasis, these bulge cells are slow cycling and quiescent in nature [36][37][38].…”

Section: Hair Follicle Stem Cellsmentioning

confidence: 99%

Molecular Mechanisms of Hair Growth and Regeneration: Current Understanding and Novel Paradigms

et al. 2020

View full text Add to dashboard Cite

Hair is a defining feature of mammals and has critical functions, including protection, production of sebum, apocrine sweat and pheromones, social and sexual interactions, thermoregulation, and provision of stem cells for skin homeostasis, regeneration, and repair. The hair follicle (HF) is considered a “mini-organ,” consisting of intricate and well-organized structures which originate from HF stem and progenitor cells. Dermal papilla cells are the main components of the mesenchymal compartments in the hair bulb and are instrumental in generating signals to regulate the behavior of neighboring epithelial cells during the hair cycle. Mesenchymal-epithelial interactions within the dermal papilla niche drive HF embryonic development as well as the postnatal hair growth and regeneration cycle. This review summarizes the current understanding of HF development, repair, and regeneration, with special focus on cell signaling pathways governing these processes. In particular, we discuss emerging paradigms of molecular signaling governing the dermal papilla-epithelial cellular interactions during hair growth and maintenance and the recent progress made towards tissue engineering of human hair follicles.

show abstract

“…(A) FACS-purified α6 + β1 + cells were sorted for (B) CD34 + Sca-1and CD34 -Sca-1 + (pink, α6 + β1 + CD34 + Sca-1 -; blue, α6 + β1 + CD34 -Sca-1 + ; orange, α6 + β1 + CD34 -Sca-1 -). Data shown is from four mice combined and sorted together (Soteriou et al, 2016). Abbreviations: HFSCs, hair follicle stem cells; FACS, fluorescence activated cell sorting.…”

Section: For Selecting Single Events Adjust Scatter Plot Ssc-a Versumentioning

confidence: 99%

“…Properly set up the FACS and all the necessary gating settings by preparing appropriate compensations, using single stained controls. In order to perform successful cell sorting, make sure to remove all the debris and accurately distinguish singlet events from doublets/aggregates (Soteriou et al, 2016). Table 1C.20.1 describes common problems, causes, and suggested solutions associated with the protocol described in this unit.…”

Section: Critical Parametersmentioning

confidence: 99%

Isolation of Stem Cells and Progenitors from Mouse Epidermis

Kostic

Sedov

Soteriou

et al. 2017

CP Stem Cell Biology

Self Cite

View full text Add to dashboard Cite

The epidermis consists of several distinct compartments including the interfollicular epidermis (IFE), sweat glands, sebaceous glands (SGs), and the hair follicle (HF). While the IFE and SGs are in a constant state of self-renewal, the HF cycles between phases of growth, destruction, and rest. The hair follicle stem cells (HFSCs) that fuel this perpetual cycle have been well described and are located in a niche termed the bulge. These bulge SCs express markers such as CD34 and Keratin 15 (K15), enabling the isolation of these cells. Here, we describe a powerful method for isolating HFSCs and epidermal progenitors from mouse skin utilizing fluorescence activated cell-sorting (FACS). Upon isolation, cells can be expanded and utilized in various in vivo and in vitro models aimed at studying the function of these unique cells. © 2017 by John Wiley & Sons, Inc.

show abstract

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Cited by 14 publications

References 29 publications

Blimp1+ cells generate functional mouse sebaceous gland organoids in vitro

Blimp1+ cells generate functional mouse sebaceous gland organoids in vitro

Molecular Mechanisms of Hair Growth and Regeneration: Current Understanding and Novel Paradigms

Isolation of Stem Cells and Progenitors from Mouse Epidermis

Contact Info

Product

Resources

About