A new, fast and sensitive method for the determination of trace amounts of nitrite using differential pulse polarography

Somer, Güler; Kalaycı, Şükrü; Almas, Ziya

doi:10.1016/j.niox.2016.05.001

Cited by 10 publications

(2 citation statements)

References 19 publications

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“…Determination of Sn(II) with Sn(IV) and determination of As(III) with As(V) can give for such examples [6] [7]. Some molecules such as ascorbic acid [8], vitamin K3 [9], uric acid [10] and nitrite [11] which are not so easy to determine with the above given methods, can also be determined with our DP polarographic methods without any difficulty.…”

Section: Introductionmentioning

confidence: 99%

Determination of Vitamin B12 Using Differential Pulse Polarography

Kalaycı¹,

Ünal²,

Somer³

2020

AJAC

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Vitamin B12 is a type of vitamin also known by the name cobalamin. B12 is involved in many metabolism activities, including DNA synthesis, nervous system, red blood formation and immune system. Therefore, we chose the Differential Pulse Polarography (DPP) method is that has a high sensitivity for the determination of vitamin B12. This determination was possible with cobalt present in vitamin B12 structure. Since Co(III) is formed from the oxidation of the vitamin, its polarographic behavior had to be determined in various electrolytes such as acetate, borate, phosphate and ammonia. The polarograms of Co(III) were taken in these electrolytes in which 1.0 M NH 3 /NH + 4 (pH = 9.8) and 1.0 M AcOH/AcO − (pH = 4.0) were found as the most suitable electrolytes. This method was successfully applied vitamin of B12 determination in a 1 mL ampoule with high precision. The LOD was found as 3.7 × 10 −7 for instead of (S/N = 3). Besides Co(III), interference effects of Zn(II), Ni(II), Cr(III), Fe(III), Cu(II), Cd(II) and Se(IV) were also studied. It was found that only Zn(II) peak had an overlap Co(III) peak in ammonium buffer. This problem could be solved by working in 1.0 M AcOH/AcO − (pH = 4.0) buffer. B12, which is 1000 µg in 1 mL vitamin ampoule, was found for 4 measurements as 999 ± 15 µg as a result of 95% confidence interval.

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Section: Introductionmentioning

confidence: 99%

Determination of Vitamin B12 Using Differential Pulse Polarography

Kalaycı¹,

Ünal²,

Somer³

2020

AJAC

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“…Although this photometric method is low cost and allows high throughput, it requires careful sample clean‐up for removal of proteins, fat and other matrix components that could interfere with the assay. Other photometric (Ensafi et al , ; Altunay et al , ) and direct electrochemical methods (Campos et al , ; Somer et al , ) share similar limitations due to matrix effects and preferential response towards nitrite only. To date, most popular methods for simultaneous determination of nitrite and nitrate are based on liquid separation.…”

Section: Introductionmentioning

confidence: 99%

A reference isotope dilution headspace GC/MS method for the determination of nitrite and nitrate in meat samples

Luckovitch

2019

Int J of Food Sci Tech

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Summary A novel method for the determination of nitrite and nitrate in meat products is presented. The samples were ground and extracted in hot water with the presence of 15NO2- and 15NO3- internal standards. The solution was buffered with sodium bicarbonate and reacted with triethyloxonium tetrafluoroborate to convert nitrite and nitrate into EtNO2 and EtONO2. Such derivatives could be detected by headspace GC/MS in positive chemical ionisation mode with 0.05 µg g−1 NO2- and 1.0 µg g−1 NO3- LOD. The method was used for NO2- and NO3- quantitation in the 0.5‐300 and 2.5‐300 µg g−1 ranges. The method was applied for the analysis of fifteen meat products. Despite minimal sample preparation, the headspace sampling ensured a clean chromatography for over 135 analyses (throughput ten samples per hour). The proposed method offers selective GC/MS detection combined with high‐precision isotope dilution calibration, it is suitable for metrological applications and can support regulations on meat safety (European Commission, 2011).

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