Global Measles and Rubella Laboratory Network Support for Elimination Goals, 2010–2015

Mulders, Mick N.; Rota, Paul A.; Icenogle, Joseph P.; Brown, Kevin E.; Takeda, Makoto; Rey, Gloria; Mamou, Myriam Corrine Ben; Dosseh, Annick; Byabamazima, Charles; Ahmed, Hinda; Pattamadilok, Sirima; Zhang, Yan; Gacic-Dobo, Marta; Strebel, Peter M.; Goodson, James L.

doi:10.15585/mmwr.mm6517a3

Cited by 56 publications

(57 citation statements)

References 4 publications

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“…Virologic surveillance in China is currently by far the most complete of any country with 497 sequences reported from China compared to only 809 sequences reported from elsewhere in the world in the last 6 years15. This study extends previous work defining lineages within genotypes 1E and 2B using Chinese RV sequences from the 739 nt region of the E1 ORF.…”

supporting

confidence: 70%

Analysis of complete genomes of the rubella virus genotypes 1E and 2B which circulated in China, 2000–2013

Zhu

Chen

Abernathy

et al. 2016

Sci Rep

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Rubella viruses of genotypes 1E and 2B are currently the most frequently detected wild-type viruses in the world. Genotype 1E viruses from China have been genetically distinct from genotype 1E viruses found elsewhere, while genotype 2B viruses found in China are not distinguishable from genotype 2B viruses from other areas. Genetic clusters of viruses of both genotypes were defined previously using sequences of the 739-nt genotyping window. Here we report phylogenic analysis using whole genomic sequences from seven genotype 1E and three genotype 2B viruses which were isolated in China between 2000 and 2013 and confirm the subgrouping of current circulating genotypes 1E and 2B viruses. In addition, the whole genomic characterization of Chinese rubella viruses was clarified. The results indicated that the Chinese rubella viruses were highly conserved at the genomic level, and no predicted amino acid variations were found at positions where functional domains of the proteins were identified. Therefore, it gives us the idea that the rubella control and elimination goal should be achieved if vaccine immunization coverage continues maintaining at the high level.

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supporting

confidence: 70%

Analysis of complete genomes of the rubella virus genotypes 1E and 2B which circulated in China, 2000–2013

Zhu

Chen

Abernathy

et al. 2016

Sci Rep

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“…To evaluate the performance of our system as a potential serosurveillance tool, we performed field testing in Kakuma, a refugee camp located in remote northwestern Kenya. The field test results were compared with reference tests [measles IgG and rubella IgG enzyme-linked immunosorbent assays (ELISAs) from Siemens Enzygnost] performed by the national laboratory at Kenya Medical Re search Institute (KEMRI), which is accredited by the WHO Global Measles-Rubella Laboratory Network (32). The results demonstrate a potential role for DMF in serosurveillance, particularly in areas where centralized laboratories are unavailable.…”

Section: Introductionmentioning

confidence: 99%

A digital microfluidic system for serological immunoassays in remote settings

et al. 2018

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“…[5][6][7][8] The World Health Organization's (WHO) Measles and Rubella Laboratory Network has recommended the collection of RUBV genotype data to support control and elimination programs globally. 9,10 This recommendation has been successfully implemented in countries that have eliminated endemic rubella and CRS, whereas in Africa this implementation is still lagging. In Cameroon, surveillance of rubella or CRS is not yet implemented.…”

mentioning

confidence: 99%

First report of the genomic characterization of rubella viruses circulating in Cameroon

Mekanda

Monamele

Nemg

et al. 2019

Journal of Medical Virology

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Rubella is an acute and contagious viral infection whose gravidity resides in infection during pregnancy, which can result in miscarriage, fetal death, stillbirth, or infants with congenital malformations. This study aimed to describe the genome of rubella viruses (RUBVs) circulating in Cameroon. Throat swabs were collected from health districts as part of the measles surveillance program from 2010 to 2016 and sent to the Centre Pasteur of Cameroon. Samples were amplified by genotyping reverse transcription polymerase chain reaction (RT‐PCR) in the search of two overlapping fragments of the gene that encodes the E1 envelope glycoprotein of RUBV. PCR products were sequenced and phylogenetic analysis was performed with MEGA 6 software. Overall, 9 of 43 samples (20.93%) were successfully amplified and sequenced but only eight sequences could be exploited for phylogenetic analysis with respect to the required fragment length of 739 nucleotides. Analysis of viral sequences from Cameroon with other epidemiologically relevant sequences from around the world showed that all RUBVs belonged to lineage L1 of genotype 1G. Cameroon sequences clustered with viruses from West Africa including Nigeria, Ivory Coast, and Ghana with a percentage similarity of 95.4% to 99.2%. This study will enable an update on the molecular epidemiology of RUBV in Cameroon and help in monitoring circulating RUBV for a better implementation of elimination strategies.

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Global Measles and Rubella Laboratory Network Support for Elimination Goals, 2010–2015

Cited by 56 publications

References 4 publications

Analysis of complete genomes of the rubella virus genotypes 1E and 2B which circulated in China, 2000–2013

Analysis of complete genomes of the rubella virus genotypes 1E and 2B which circulated in China, 2000–2013

A digital microfluidic system for serological immunoassays in remote settings

First report of the genomic characterization of rubella viruses circulating in Cameroon

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