In Vitro Cytotoxic Activity of Origanum vulgare L. on HCT-116 and MDA-MB-231 Cell Lines

Abstract

In the present investigation, we examined the cytotoxic effect of methanolic extract from Origanum vulgare on HCT-116 and MDA-MB-231 cell line in vitro. In order to determine the cytotoxic effects we used an MTT viability assay. The results showed that cell growth is significantly lower in extract treated cells compared to untreated control. The effect of inhibition of cell growth was higher in the treatment of HCT-116 cell line than in MDA-MB-231. Based on the results it is determined that O. vulgare is a significant source of biologically active substances that have cytotoxic and antiproliferative activity in vitro.

LC‐MS‐based metabolite profiling of methanolic extracts from the medicinal and aromatic species Mentha pulegium and Origanum majorana

“…majoranum (Hussain and Markham, ) as well as O . vulgare (Grbović et al ., ; Martins et al ., ). Peaks 42 and 44 were characterised as isoorientin and orientin, with ions at m / z 447, showing the same fragmentation pattern at m / z 357 and 327, which correspond to [M−H−90] − and [M−H−120] − , respectively, from the main fragment.…”

“…It exhibited a fragment at m/z 473, which corresponds to the loss of the [MÀHÀ90À120] À ion from the precursor ion. Vicenin-2 was reported to occur in O. majoranum (Hussain and Markham, 1981) as well as O. vulgare (Grbović et al, 2013;Martins et al, 2014). Peaks 42 and 44 were characterised as isoorientin and orientin, with ions at m/z 447, showing the same fragmentation pattern at m/z 357 and 327, which correspond to [MÀHÀ90] À and [MÀHÀ120] À , respectively, from the main fragment.…”

Cytotoxic Effect of Rosmarinus officinalis L. Leaf Extracts on Tumor Cell Line

“…There is a great need to examine reliable and inexhaustible sources of natural substances. In addition, it is important to understand the mechanisms of anticancer agents for future application in cancer therapy [15]. Carcinogenesis is one of the most important phenomena that could be attributed to free radicals/reactive oxygen species.…”

Evaluation of the Antioxidant Activities and Cytotoxicities of Selected Medicinal Herbs Using Human Hepatoma Cell Line (Hepg2)

“…Based on National Cancer Institute Standard (IC50>100), P. frainifolia and O. vulgare extracts can be considered as "non-toxic" to cells [30]. According to the published reports about the cytotoxicity of O. vulgare extract and also the minor difference between IC50 (107.6 µg/ml) and the toxicity threshold level for P. frainifolia obtained in this study, extreme caution should be taken before using these plants as natural antioxidants [31,32].…”