Overexpression of a Gene Involved in Phytic Acid Biosynthesis Substantially Increases Phytic Acid and Total Phosphorus in Rice Seeds

Tagashira, Yusuke; Shimizu, Tomoe; Miyamoto, Masanobu; Nishida, Sho; Yoshida, Kaoru

doi:10.3390/plants4020196

Cited by 23 publications

(15 citation statements)

References 23 publications

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“…The increased rate of phenological development with increasing P fertilization has been seen in other studies on different crops (Amanullah and Khan, 2010 ; Khalil et al, 2010 ; Amanullah et al, 2010b , 2014 ; Amanullah, 2011 ; Amanullah and Khalid, 2015 ). As P deficiency can decrease both root and shoot development (Amanullah and Stewart, 2013 ) and phosphorus uptake (Amanullah and Inamullah, 2016b ), which can cause a delay in phenological development (Amanullah et al, 2014 ; Amanullah and Khalid, 2015 ; Amanullah et al, 2016b ).…”

Section: Discussionsupporting

confidence: 70%

“…Phosphorus is the second most required plant nutrient for field crops. It plays a vital role in several physiological processes of plants (Mandal and Khan, 1972 ; Singh and Sale, 2000 ; Chang et al, 2007 ; Amanullah, 2011 ; Vahed et al, 2012 ). It is always inadequate under rice based system in Northwest Pakistan to meet the requirement of rice plants (Amanullah and Inamullah, 2016a , b ).…”

Section: Introductionmentioning

confidence: 99%

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Growth and Productivity Response of Hybrid Rice to Application of Animal Manures, Plant Residues and Phosphorus

et al. 2016

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The objective of this research was to evaluate the impact of organic sources (animal manures vs. plant residues at the rate of 10 t ha−1 each) on the productivity of hybrid rice (Oryza sativa L.) production under different levels of phosphorus (0, 30, 60, and 90 kg P ha−1) fertilization. Two separate field experiments were conducted. In experiment (1), impact of three animal manures sources (cattle, sheep, and poultry manures) and P levels were studied along with one control plot (no animal manure and P applied) was investigated. In experiment (2), three plant residues sources (peach leaves, garlic residues, and wheat straw) and P levels were studied along with one control plot (no plant residues and P applied). Both the experiments were carried out on small land farmer field at District Swabi, Khyber Pakhtunkhwa Province (Northwest Pakistan) during summer 2015. The results revealed that in both experiments the control plot had significantly (p ≤ 0.05) less productivity than the average of all treated plots with organic sources and P level. The increase in P levels in both experiments (animal manure vs. plant residues) resulted in higher rice productivity (90 > 60 > 30 > 0 kg P ha−1). In the experiment under animal manures, application of poultry manure increased rice productivity as compared with sheep and cattle manures (poultry > sheep > cattle manures). In the experiment under plant residues, application of peach leaves or garlic residues had higher rice productivity than wheat straw (peach leaves = garlic residues > wheat straw). On average, rice grown under animal manures produced about 20% higher grain yield than rice grown under crop residues. We conclude from this study that application of 90 kg P ha−1 along with combined application of animal manures, especially poultry manure increases rice productivity. Also, the use of either garlic residues or peach leaves, never applied before as organic manures, can increase crop productivity and will help in degraded soil for sustainable soil management.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Growth and Productivity Response of Hybrid Rice to Application of Animal Manures, Plant Residues and Phosphorus

et al. 2016

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“…Pi homeostasis. Overexpression of PA biosynthesis genes in rice resulted in an enhanced influx of P from vegetative organs into seeds41. These observations support the hypothesis for the continuous uptake of Pi that is translocated to aleurone layer, where it is rapidly utilized for the biosynthesis of PA.…”

Section: Discussionsupporting

confidence: 81%

Tissue specific transcript profiling of wheat phosphate transporter genes and its association with phosphate allocation in grains

Shukla

Kaur

Aggarwal

et al. 2016

Sci Rep

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Approaches enabling efficient phosphorus utilization in crops are of great importance. In cereal crop like wheat, utilization of inorganic phosphate (Pi) is high and mature grains are the major sink for Pi utilization and storage. Research that addresses the importance of the Pi homeostasis in developing grains is limited. In an attempt to understand the Pi homeostasis in developing wheat grains, we identified twelve new phosphate transporters (PHT), these are phyologentically well distributed along with the members reported from Arabidopsis and rice. Enhanced expression of PHT1-subfamily genes was observed in roots subjected to the Pi starvation suggesting their active role in Pi homeostasis. Differential expression patterns of all the PHT genes during grain filling stages suggested their importance in the filial tissues. Additionally, high accumulation of Pi and total P in aleurone correlates well with the expression of TaPHTs and other phosphate starvation related genes. Tissue specific transcript accumulation of TaPHT1.1, TaPHT1.2, TaPHT1.4 in aleurone; TaPHT3.1 in embryo and TaPHT4.2 in the endosperm was observed. Furthermore, their transcript abundance was affected in low phytate wheat grains. Altogether, this study helps in expanding the knowledge and prioritize the candidate wheat Pi-transporters to modulate the Pi homeostasis in cereal grains.

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“…The two members of the ABCB gene family in Arabidopsis (AtABCB1 and AtABCB2) are auxin transporters and the overexpression of AtABCB1 causes the elongation of hypocotyl cells [11,12]. Several members of the ABCC subfamily are responsible for phytate transport as exempli ed in Arabidopsis (AtABCC5) [13], maize (ZmABCC4) [14] and rice (OsABCC13) [15]. Two other ABCC transporters (AtABCC1 and AtABCC2) mediate tolerance to both cadmium (Cd) and mercury by vacuolar sequestration [16].…”

Section: Page 3/21mentioning

confidence: 99%

Genome-wide Identification of ATP Binding Cassette (ABC) Transporter and Heavy Metal Associated (HMA) Gene Families in Flax (Linum usitatissimum L.)

Khan

You

Datla

et al. 2020

Preprint

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BackgroundThe recent release of the reference genome sequence assembly of flax, a self-pollinated crop with 15 chromosome pairs, into chromosome-scale pseudomolecules enables the characterization of gene families. The ABC transporter and HMA gene families are important in the control of cadmium (Cd) accumulation in crops. To date, the genome-wide analysis of these two gene families has been successfully conducted in some plant species, but no systematic evolutionary analysis is available for the flax genome. ResultsHere we describe the ABC transporter and HMA gene families in flax to provide a comprehensive overview of its evolution and some support towards the functional annotation of its members. The 198 ABC transporter and 12 HMA genes identified in the flax genome were classified into eight ABC transporter and four HMA subfamilies based on their phylogenetic analysis and domains’ composition. Nine of these genes, i.e., LuABCC9, LuABCC10, LuABCG58, LuABCG59, LuABCG71, LuABCG72, LuABCG73, LuHMA3, and LuHMA4, were orthologous with the Cd associated genes in Arabidopsis, rice and maize. Ten motifs were identified from all ABC transporter and HMA genes. Also, several motifs were conserved among genes of similar length, but subfamilies each had their own motif structures. Both the ABC transporter and HMA gene families were highly conserved among subfamilies of flax and with those of Arabidopsis. While four types of gene duplication were observed at different frequencies, whole-genome or segmental duplications were the most frequent with 162 genes, followed by 29 dispersed, 14 tandem and 4 proximal duplications, suggesting that segmental duplications contributed the most to the expansion of both gene families in flax. The rates of non-synonymous to synonymous (Ka/Ks) mutations of paired duplicated genes were for the most part lower than one, indicative of a predominant purifying selection. Only five pairs of genes clearly exhibited positive selection with a Ka/Ks ratio greater than one. Gene ontology analyses suggested that most flax ABC transporter and HMA genes had a role in ATP binding, transport, catalytic activity, ATPase activity, and metal ion binding. The RNA-Seq analysis of eight different organs demonstrated diversified expression profiling patterns of the genes and revealed their functional or sub-functional conservation and neo-functionalization. ConclusionCharacterization of the ABC transporter and HMA gene families will help in the functional analysis of candidate genes in flax and other crop species.

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Overexpression of a Gene Involved in Phytic Acid Biosynthesis Substantially Increases Phytic Acid and Total Phosphorus in Rice Seeds

Cited by 23 publications

References 23 publications

Growth and Productivity Response of Hybrid Rice to Application of Animal Manures, Plant Residues and Phosphorus

Growth and Productivity Response of Hybrid Rice to Application of Animal Manures, Plant Residues and Phosphorus

Tissue specific transcript profiling of wheat phosphate transporter genes and its association with phosphate allocation in grains

Genome-wide Identification of ATP Binding Cassette (ABC) Transporter and Heavy Metal Associated (HMA) Gene Families in Flax (Linum usitatissimum L.)

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