The role of transketolase and octulose in the resurrection plant<i>Craterostigma plantagineum</i>

Zhang, Qingwei; Linnemann, Thomas; Schreiber, Lukas; Bartels, Dorothea

doi:10.1093/jxb/erw174

Cited by 11 publications

(7 citation statements)

References 43 publications

(59 reference statements)

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“…Craterostigma and L. brevidens accumulate the unusual C8 sugar 2-octulose in photosynthetic tissues, which serves as a reservoir of sucrose accumulation during desiccation (Bianchi et al, 1991;Phillips et al, 2008). Transketolase7 and -10 catalyze the formation of octulose-8-phosphate in Craterostigma (Zhang et al, 2016), and the orthologous transketolase genes were highly expressed in L. brevidens leaf tissue ( Supplemental Table 10). This included two pairs of syntenic 1:1 orthologs and a trio of retained 2:1 duplicates, with upregulation in well-watered and rehydrating tissue in L. brevidens and no or little expression in L. subracemosa.…”

Section: Unique Desiccation-related Pathways In L Brevidensmentioning

confidence: 99%

Desiccation Tolerance Evolved through Gene Duplication and Network Rewiring in Lindernia

et al. 2018

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Although several resurrection plant genomes have been sequenced, the lack of suitable dehydration-sensitive outgroups has limited genomic insights into the origin of desiccation tolerance. Here, we utilized a comparative system of closely related desiccation-tolerant (Lindernia brevidens) and -sensitive (Lindernia subracemosa) species to identify gene-and pathwaylevel changes associated with the evolution of desiccation tolerance. The two high-quality Lindernia genomes we assembled are largely collinear, and over 90% of genes are conserved. L. brevidens and L. subracemosa have evidence of an ancient, shared whole-genome duplication event, and retained genes have neofunctionalized, with desiccation-specific expression in L. brevidens. Tandem gene duplicates also are enriched in desiccation-associated functions, including a dramatic expansion of early light-induced proteins from 4 to 26 copies in L. brevidens. A comparative differential gene coexpression analysis between L. brevidens and L. subracemosa supports extensive network rewiring across early dehydration, desiccation, and rehydration time courses. Many LATE EMBRYOGENESIS ABUNDANT genes show significantly higher expression in L. brevidens compared with their orthologs in L. subracemosa. Coexpression modules uniquely upregulated during desiccation in L. brevidens are enriched with seed-specific and abscisic acid-associated cis-regulatory elements. These modules contain a wide array of seed-associated genes that have no expression in the desiccation-sensitive L. subracemosa. Together, these findings suggest that desiccation tolerance evolved through a combination of gene duplications and network-level rewiring of existing seed desiccation pathways.

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Section: Unique Desiccation-related Pathways In L Brevidensmentioning

confidence: 99%

Desiccation Tolerance Evolved through Gene Duplication and Network Rewiring in Lindernia

et al. 2018

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“…Thus, only further identification of the cellular localization and properties of enzymes with multiple roles can help to understand the contribution of these enzymes to desiccation tolerance. The studies on transketolase of C. plantagineum from Bernacchia et al (1995), Willige et al (2009), and Zhang et al (2016) provide an example for this proposal: three isoforms of transketolase (tkt3, tkt7, and tkt10) have been characterized on the molecular level in C. plantagineum ; tkt3 is localized in chloroplasts while tkt7 and tkt10 are localized in the cytoplasm; tkt3 is suggested to be involved in carbon reactions in photosynthesis and the pentose phosphate pathway, while tkt7 and tkt10 are involved in the accumulation of octulose in C. plantagineum [67,68,69]. …”

Section: Sugar Metabolism During Dehydration Studied By Proteomicsmentioning

confidence: 99%

“…Octulose in Craterostigma species may have a similar role as starch in providing carbon resources for sucrose or RFOs synthesis [26,70]. Zhang et al (2016) demonstrated that C. plantagineum tkt7 and tkt10 catalyze the synthesis of octulose-8-phosphate using glucose-6-phosphate and fructose-6-phosphate as substrates [69]. Besides starch degradation, dehydration also induces alterations of the polysaccharide contents and structure in the cell wall of resurrection plants [75].…”

Section: The Relationships Among Sugar Accumulation Energy Metabomentioning

confidence: 99%

“…To prevent damage associated with oxidative stress, resurrection plants have evolved various protective mechanisms [82]. Many resurrection plants, such as L. brevidens [69] or M. flabellifolia [68] will accumulate anthocyanins that have a basic structure composed of carbon and act as photoprotective light screens in vegetative tissues [83]. To respond to ROS produced by dehydration stress, plants induce antioxidant defense systems.…”

Section: The Relationships Among Sugar Accumulation Energy Metabomentioning

confidence: 99%

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Enzymes and Metabolites in Carbohydrate Metabolism of Desiccation Tolerant Plants

2016

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Resurrection plants can tolerate extreme water loss. Substantial sugar accumulation is a phenomenon in resurrection plants during dehydration. Sugars have been identified as one important factor contributing to desiccation tolerance. Phylogenetic diversity of resurrection plants reflects the diversity of sugar metabolism in response to dehydration. Sugars, which accumulate during dehydration, have been shown to protect macromolecules and membranes and to scavenge reactive oxygen species. This review focuses on the performance of enzymes participating in sugar metabolism during dehydration stress. The relation between sugar metabolism and other biochemical activities is discussed and open questions as well as potential experimental approaches are proposed.

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“…The enzyme transketolase is a limiting factor of photosynthesis, and small changes in its activity can lead to the inhibition of plant growth as well as phenylalanine and aromatic amino acid metabolism [ 3 , 4 , 5 , 6 ]. Since transketolase plays a key role in plant photosynthesis, and is highly conserved in plants, it represents an important candidate target for the development of new herbicides.…”

Section: Introductionmentioning

confidence: 99%

Structure-Based Discovery and Synthesis of Potential Transketolase Inhibitors

et al. 2018

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Transketolase (TKL) plays a key role in plant photosynthesis and has been predicted to be a potent herbicide target. Homology modeling and molecular dynamics simulation were used to construct a target protein model. A target-based virtual screening was developed to discover novel potential transketolase inhibitors. Based on the receptor transketolase 1 and a target-based virtual screening combined with structural similarity, six new compounds were selected from the ZINC database. Among the structural leads, a new compound ZINC12007063 was identified as a novel inhibitor of weeds. Two novel series of carboxylic amide derivatives were synthesized, and their structures were rationally identified by NMR and HRMS. Biological evaluation of the herbicidal and antifungal activities indicated that the compounds 4u and 8h were the most potent herbicidal agents, and they also showed potent fungicidal activity with a relatively broad-spectrum. ZINC12007063 was identified as a lead compound of potential transketolase inhibitors, 4u and 8h which has the herbicidal and antifungal activities were synthesized based on ZINC12007063. This study lays a foundation for the discovery of new pesticides.

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The role of transketolase and octulose in the resurrection plantCraterostigma plantagineum

Abstract: HighlightTransketolase 7 and 10 of Craterostigma plantagineum participate in the synthesis of octulose phosphate in an alternative Calvin cycle. Octulose is the main transport sugar in fully hydrated plants.

Cited by 11 publications

References 43 publications

Desiccation Tolerance Evolved through Gene Duplication and Network Rewiring in Lindernia

Desiccation Tolerance Evolved through Gene Duplication and Network Rewiring in Lindernia

Enzymes and Metabolites in Carbohydrate Metabolism of Desiccation Tolerant Plants

Structure-Based Discovery and Synthesis of Potential Transketolase Inhibitors

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