Substantial and robust changes in microRNA transcriptome support postnatal development of the hypothalamus in rat

Doubi-Kadmiri, Soraya; Benoît, Charlotte; Benigni, Xavier; Beaumont, Guillaume; Vacher, Claire-Marie; Taouis, Mohammed; Baroin-Tourancheau, Anne; Amar, Laurence

doi:10.1038/srep24896

Cited by 19 publications

(14 citation statements)

References 26 publications

(27 reference statements)

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“…Individual cDNA libraries were built following an Illumina-like protocol in which 3′- and 5′-adapters were sequentially ligated at the 3′- and 5′-ends of small RNAs, respectively, to allow for their reverse transcription (RT) and amplification by polymerase chain reaction (PCR) as previously described (Doubi-Kadmiri et al, 2016 ). The 3′-Adaptor was first adenylated by using 25 pmoles of oligonucleotides, 2 × Quick Ligation reaction buffer (New England Biolabs), and 1,600 U of T4 DNA ligase (New England Biolabs), in a volume of 50 μl and by overnight incubation at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Different miRNAs have been related to peripheral controls of metabolisms (Vienberg et al, 2017 ). miRNA involvement in such functions at the ARC level has started to be explored and the possibility that miRNAs of ARC participate in the central control of energy homeostasis has yet to be characterized (Amar et al, 2012 ; Baroin-Tourancheau et al, 2014 ; Doubi-Kadmiri et al, 2016 ). Here, we have identified miRNA expression responses of ARC of adult male rats that had been submitted to perinatal and/or adult metabolic environmental challenges and consequently displayed differences in circulating levels of nutrients and hormones.…”

Section: Introductionmentioning

confidence: 99%

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miRNA Long-Term Response to Early Metabolic Environmental Challenge in Hypothalamic Arcuate Nucleus

Benoît¹,

Doubi-Kadmiri²,

Benigni³

et al. 2018

Front. Mol. Neurosci.

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Epidemiological reports and studies using rodent models indicate that early exposure to nutrient and/or hormonal challenges can reprogram metabolism at adulthood. Hypothalamic arcuate nucleus (ARC) integrates peripheral and central signals to adequately regulate energy homeostasis. microRNAs (miRNAs) participate in the control of gene expression of large regulatory networks including many signaling pathways involved in epigenetics regulations. Here, we have characterized and compared the miRNA population of ARC of adult male rats continuously exposed to a balanced metabolic environment to the one of adult male rats exposed to an unbalanced high-fat/high-carbohydrate/moderate-protein metabolic environment during the perinatal period and/or at adulthood that consequently displayed hyperinsulinemia and/or hyperleptinemia. We identified more than 400 miRNA species in ARC of adult male rats. By comparing the miRNA content of six biological replicates in each of the four perinatal/adult environments/rat groups, we identified the 10 miRNAs specified by clusters miR-96/182/183, miR-141/200c, and miR-200a/200b/429 as miRNAs of systematic and uncommonly high variation of expression. This uncommon variation of expression may underlie high individual differences in aging disease susceptibilities. By comparing the miRNA content of the adult ARC between the rat groups, we showed that the miRNA population was not affected by the unbalanced adult environment while, in contrast, the expression of 11 miRNAs was repeatedly impacted by the perinatal unbalanced environment. Our data revealed a miRNA response of adult ARC to early metabolic environmental challenge.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

miRNA Long-Term Response to Early Metabolic Environmental Challenge in Hypothalamic Arcuate Nucleus

Benoît¹,

Doubi-Kadmiri²,

Benigni³

et al. 2018

Front. Mol. Neurosci.

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“…An epigenetic switch regulating the post‐transcriptional control of gene expression mediated by miRNAs has been described in inflammation‐related pathologies and it has even been proposed that lncRNA, which can code for miRNA, is an emergent modulator of the transcriptional activity of inflammatory response‐related genes . In the developing hypothalamus, important changes in the expression pattern of miRNAs have been described, suggesting the involvement of miRNAs in the functional organisation of the melanocortin system . Also, there is evidence showing that leptin, a hormone whose signal transduction pathway is downregulated in DIHI, modulates the hypothalamic expression of miRNAs and, conversely, miRNAs could modulate leptin signalling .…”

Section: New Cellular Mechanisms Involved In the Development Of Hypotmentioning

confidence: 99%

Cell and molecular mechanisms behind diet‐induced hypothalamic inflammation and obesity

Ávalos

Kerr

Maliqueo

et al. 2018

J Neuroendocrinology

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Diet-induced obesity (DIO) is associated with chronic, low-grade inflammation in the hypothalamus, a key regulator of energy homeostasis. Current studies have revealed the involvement of different cell types, as well as cell and molecular mechanisms, that contribute to diet-induced hypothalamic inflammation (DIHI) and DIO. Subsequent to the discovery that high-fat diet and saturated fatty acids increase the expression of hypothalamic cytokines prior to weight gain, research has focused on understanding the cellular and molecular mechanisms underlying these changes, in addition to the role of inflammation in the pathogenesis of obesity. Recent studies have proposed that the inhibition of pro-inflammatory pathways in microglia and astrocytes is sufficient to protect against DIHI and prevent obesity. In addition, impairment of intracellular and epigenetic mechanisms, such as hypothalamic autophagy and changes in the methylation pattern of certain genes, have been implicated in susceptibility to DIHI and DIO. Interestingly, a sexual dimorphism has been found during DIO in hypothalamic inflammation, glial activation and metabolic diseases, and recent data support an important role of sex steroids in DIHI. These new exciting findings uncover novel obesity pathogenic mechanisms and provide targets to develop therapeutic approaches.

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“…For instance, Zhang et al showed that 30 miRNAs including miR-7 and miR-191 are differentially expressed in the hypothalamus of the pig between stages P60, P120, and/or P180 (Zhang et al, 2013). More recently, a nice work showed robust changes in the expression of numerous miRNAs during the period of functional organization of the ARC and median eminence between stages P8–P14 and stages P21–P28 (Doubi-Kadmiri et al, 2016). …”

Section: The Microrna and The Melanocortin Systemmentioning

confidence: 99%

The Role of MicroRNA in the Modulation of the Melanocortinergic System

et al. 2017

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The central control of energy balance involves a highly regulated neuronal network within the hypothalamus and the dorsal vagal complex. In these structures, pro-opiomelanocortin (POMC) neurons are known to reduce meal size and to increase energy expenditure. In addition, leptin, a peripheral signal that relays information regarding body fat content, modulates the activity of melanocortin pathway neurons including POMC-, Agouti-related peptide (AgRP)/Neuropeptide Y (NPY)-, melanocortin receptors (MC3R and MC4R)-expressing neurons. MicroRNAs (miRNAs) are short non-coding RNAs of 22–26 nucleotides that post-transcriptionally interfere with target gene expression by binding to their mRNAs. Evidence has demonstrated that miRNAs play important roles in the central regulation of energy balance. In this context, different studies identified miRNAs including miR-200 family, miR-103, or miR-488 that could target the genes of melanocortin pathway. More precisely, these different miRNAs can modulate energy homeostasis by affecting leptin transduction pathway in the POMC, or AgRP/NPY neurons. This article reviews the role of identified miRNAs in the modulation of melanocortin pathway in the context of energy homeostasis.

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Substantial and robust changes in microRNA transcriptome support postnatal development of the hypothalamus in rat

Cited by 19 publications

References 26 publications

miRNA Long-Term Response to Early Metabolic Environmental Challenge in Hypothalamic Arcuate Nucleus

miRNA Long-Term Response to Early Metabolic Environmental Challenge in Hypothalamic Arcuate Nucleus

Cell and molecular mechanisms behind diet‐induced hypothalamic inflammation and obesity

The Role of MicroRNA in the Modulation of the Melanocortinergic System

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