Placental aging and oxidation damage in a tissue micro-array model: an immunohistochemistry study

Londero, Ambrogio P.; ­Orsaria, Maria; Marzinotto, Stefania; Grassi, Tiziana; Fruscalzo, Arrigo; Calcagno, Angelo; Bertozzi, Serena; Nardini, Nastassia; Stella, Enrica; Lellé, Ralph J.; Driul, Lorenza; Tell, Gianluca; Mariuzzi, Laura

doi:10.1007/s00418-016-1435-6

Cited by 48 publications

(48 citation statements)

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“…Interestingly, we also observed in COUP-TFI −/− mice a level of p53 expression that was lower than its downstream target p21 and that the expression rate of the two genes positively correlated. This data con rmed previous data on human placentas (20). We could further hypothesize a role of p21 independent from p53.…”

Section: Discussionsupporting

confidence: 91%

“…In particular, we analyzed the expression of Hif1α, ENG, Flt1, PlGF, VEGF-A, which are main players in angiogenesis and vascular path nding, P21 and P53 in cellular proliferation, as well as Bax, Bcl2 and INHA, which are involved in apoptosis and cell survival. All these genes regulate crucial aspects of placental development, and their variation was shown to be associated with impaired development of the ongoing pregnancy (19,20).…”

Section: Discussionmentioning

confidence: 99%

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COUP-TFI Deletion Affects Angiogenesis and Apoptosis Related Genes Expression in Ex-Vivo Mice Placenta

Viola¹,

Marzinotto²,

Bertacchi³

et al. 2020

Preprint

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Background: Chicken Ovalbumin Upstream Promoter-Transcription Factor I (COUP-TFI) is a member of the steroid/thyroid nuclear receptor superfamily. Aim of the study was to investigate whether the absence of this gene affects placental development and fetal growth in a mouse model. For this scope placentas of COUP-TFI-knockout (COUP-TFI –/–) and wild-type (WT) were collected at the 18.5 days post-coitum. mRNA amount of following genes known to be involved in different key molecular pathways were evaluated: Bax and Bcl-2 (apoptosis), p21 and p53 (proliferation), VEGF-A, PlGF, HIF1α, Flt-1, ENG, and INHA (angiogenesis). Mice litter weight at birth was also compared.Results: RT-qPCR analysis showed an increased mRNA expression of VEGF-A and Bax in placental tissue of COUP-TFI –/– mice compared to WT mice. Also, a lost in the positive correlation between Bcl-2 and INHA, p21 and ENG, as well as HIF1α and Flt-1 mRNA expression in COUP-TFI null mutants was found. Finally, the mean weight of WT mice was 1.6 g (±0.14) compared to 1.3 grams (±0.13) of COUP-TFI –/– mice (p<0.05). Conclusions: Our results show that COUP-TFI deletion is associated to a lower birth weight in mouse and an increased placental mRNA expression of pro-apoptotic Bax and pro-angiogenetic VEGF-A genes.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

COUP-TFI Deletion Affects Angiogenesis and Apoptosis Related Genes Expression in Ex-Vivo Mice Placenta

Viola¹,

Marzinotto²,

Bertacchi³

et al. 2020

Preprint

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“…fusion and aggregation of telomeric DNA. 1,38,39 Telomere length is regulated by the enzyme telomerase, a specific reverse transcriptase, capable of adding telomeric repeats to the ends of the chromosome. 40 Telomerase consists of a catalytic protein component, telomerase reverse transcriptase (TERT) and an RNA template component, tel-…”

Section: Cellular Senescence and Ageingmentioning

confidence: 99%

“…Each organ within an organism also exhibits ageing-related changes; the placenta is no exception. The placenta, a specialized organ formed during pregnancy, grows throughout gestation, performs multiple functions, including endocrine regulation and nourishment of the foetus, 1 but also ages and is discarded at the end of pregnancy, while the foetus may live for another hundred years. So placental ageing is a normal physiologic phenomenon.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress, placental ageing‐related pathologies and adverse pregnancy outcomes

Sultana

Maiti

Aitken

et al. 2017

American J Rep Immunol

210

196

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Oxidative stress (OS), an imbalance between free radical generation and antioxidant defence, is recognized as a key factor in the pathogenesis of adverse pregnancy outcomes. Although OS is a common future of normal pregnancy, persistent, overwhelming OS leads to consumption and decline of antioxidants, affecting placental antioxidant capacity and reducing systems. The accumulation of OS causes damage to lipids, proteins and DNA in the placental tissue that induces a form of accelerated ageing.Premature ageing of the placenta is associated with placental insufficiency that prevents the organ meeting the needs of the foetus, and as a consequence, the viability of the foetus is compromised. This review summarizes the literature regarding the role of OS and premature placental ageing in the pathophysiology of pregnancy complications. K E Y W O R D Sintrauterine growth restriction, oxidative stress, placental ageing, pre-eclampsia, preterm birth, senescence, stillbirth | INTRODUCTIONAll living organisms have limited life cycles, and ageing is part of that life cycle. Each organ within an organism also exhibits ageing-related changes; the placenta is no exception. The placenta, a specialized organ formed during pregnancy, grows throughout gestation, performs multiple functions, including endocrine regulation and nourishment of the foetus, 1 but also ages and is discarded at the end of pregnancy, while the foetus may live for another hundred years. So placental ageing is a normal physiologic phenomenon. 2 However, there are likely to be some placentas which show signs of ageing earlier than others, in the same way as some individuals age more quickly than others. Premature ageing and degenerative changes in the placenta may reduce the functional capacity of the placenta and lead to abnormal pregnancy outcomes. The placenta is the primary organ for transferring nutrients from the mother to the foetus, so growth and function of the placenta are precisely regulated and coordinated to ensure the optimal growth and development of the foetus. The placenta exchanges nutrients, for example oxygen, amino acids, carbohydrates, minerals and waste products, for example carbon dioxide between the maternal and foetal circulatory systems. 3 It releases hormones into both the maternal and foetal circulations to affect uterine function, maternal metabolism, foetal growth and development. Moreover, it metabolizes some substances and can release metabolic products into both foetal and maternal circulations. The placenta can help to protect the foetus against certain xenobiotic molecules, infections and maternal diseases. Therefore, the adequate function of this organ is crucial for a normal physiologic gestational process and a healthy baby as a final outcome.In this review, we focus on the role of OS in the pathophysiology of pregnancy complications, beginning with a brief overview of placental development at different stages of gestation. We then discuss the biochemical markers of ageing and OS-induced placental ageing.Finally, we disc...

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“…Reactive oxygen species, of high importance in a wide variety of pathologies, have been implicated in DNA damage, resulting in cellular senescence. Londero et al (2016) analyzed semiquantitatively immunohistochemically stained tissue microarrays from placental tissue derived from healthy and pathologic pregnancies including those from preeclampsia, HELLP syndrome, small for gestational age fetuses and intrauterine growth restriction. This exhaustive In this issue of HCB, we introduce a new editorial feature we are calling "In Focus in HCB."…”

mentioning

confidence: 99%

In Focus in HCB

Taatjes

Roth

2016

Histochem Cell Biol

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Placental aging and oxidation damage in a tissue micro-array model: an immunohistochemistry study

Cited by 48 publications

References 48 publications

COUP-TFI Deletion Affects Angiogenesis and Apoptosis Related Genes Expression in Ex-Vivo Mice Placenta

COUP-TFI Deletion Affects Angiogenesis and Apoptosis Related Genes Expression in Ex-Vivo Mice Placenta

Oxidative stress, placental ageing‐related pathologies and adverse pregnancy outcomes

In Focus in HCB

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