Epithelial Cell Transforming 2 and Aurora Kinase B Modulate Formation of Stress Granule–Containing Transcripts from Diverse Cellular Pathways in Astrocytoma Cells

Weeks, Adrienne; Agnihotri, Sameer; Lymer, Jennifer; Chalil, Alan; Diaz, Roberto J.; Işık, Semra; Smith, Christian A.; Rutka, James T.

doi:10.1016/j.ajpath.2016.02.013

Cited by 9 publications

(9 citation statements)

References 63 publications

(76 reference statements)

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“…The percentage of various studies is shown in Figure 2. Meanwhile, the majority of studies, about 66% of studies, are dedicated to cell culture studies only (Baguet et al, 2007;Miyoshi et al, 2007;Arimoto et al, 2008;Eisinger-Mathason et al, 2008;Goulet et al, 2008;Busà et al, 2010;Gottschald et al, 2010;Guo et al, 2010;Kalra et al, 2010;Nikpour et al, 2011;Taniuchi et al, 2011aTaniuchi et al, ,b, 2014Park et al, 2012;Fournier et al, 2013;Pizzo et al, 2013;Thedieck et al, 2013;Chang et al, 2014;Kano et al, 2014;Podszywalow-Bartnicka et al, 2014;Yuan et al, 2014;Liu et al, 2015;Somasekharan et al, 2015;Szafron et al, 2015;Valentin-Vega et al, 2016;Weeks et al, 2016;Narayanan et al, 2017;Wall and Lewis, 2017;Takayama et al, 2018;Haghandish et al, 2019;Heberle et al, 2019;Kashiwagi et al, 2019;Mazloomian et al, 2019;Cui et al, 2020;Do et al, 2020;Brown et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Stress Granules Involved in Formation, Progression and Metastasis of Cancer: A Scoping Review

Asadi

Rahmanpour

Moslehian

et al. 2021

Front. Cell Dev. Biol.

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The assembly of stress granules (SGs) is a well-known cellular strategy for reducing stress-related damage and promoting cell survival. SGs have become important players in human health, in addition to their fundamental role in the stress response. The critical role of SGs in cancer cells in formation, progression, and metastasis makes sense. Recent researchers have found that several SG components play a role in tumorigenesis and cancer metastasis via tumor-associated signaling pathways and other mechanisms. Gene-ontology analysis revealed the role of these protein components in the structure of SGs. Involvement in the translation process, regulation of mRNA stability, and action in both the cytoplasm and nucleus are among the main features of SG proteins. The present scoping review aimed to consider all studies on the effect of SGs on cancer formation, proliferation, and metastasis and performed based on a six-stage methodology structure and the PRISMA guideline. A systematic search of seven databases for qualified articles was conducted before July 2021. Publications were screened, and quantitative and qualitative analysis was performed on the extracted data. Go analysis was performed on seventy-one SGs protein components. Remarkably G3BP1, TIA1, TIAR, and YB1 have the largest share among the proteins considered in the studies. Altogether, this scoping review tries to demonstrate and provide a comprehensive summary of the role of SGs in the formation, progression, and metastasis of cancer by reviewing all studies.

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Section: Resultsmentioning

confidence: 99%

Stress Granules Involved in Formation, Progression and Metastasis of Cancer: A Scoping Review

Asadi

Rahmanpour

Moslehian

et al. 2021

Front. Cell Dev. Biol.

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“…Our previous work demonstrated that SGs could form in GBM stem cells and culture in vitro 37 . Here we have demonstrated for the first time the presence of cytoplasmic aggregates reminiscent of SGs in operative human GBM tissues.…”

Section: Discussionmentioning

confidence: 99%

“…During stress, mTOR function is diminished to conserve cellular energy 35,36 . We previously demonstrated that mTOR effectors are contained in SGs 37 and therefore postulated that mTOR activity would be low in U251 cells with sustained SGs. To ascertain the status of mTOR signaling in our model, we examined the phosphorylation level of the mTOR effector rpS6 in raloxifene-treated cells post-hypoxia.…”

Section: Raloxifene Prevents Re-activation Of Mtor Signaling Posthypoxiamentioning

confidence: 99%

Raloxifene prevents stress granule dissolution, impairs translational control and promotes cell death during hypoxia in glioblastoma cells

et al. 2020

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Glioblastoma (GBM) is the most common primary malignant brain tumor, and it has a uniformly poor prognosis. Hypoxia is a feature of the GBM microenvironment, and previous work has shown that cancer cells residing in hypoxic regions resist treatment. Hypoxia can trigger the formation of stress granules (SGs), sites of mRNA triage that promote cell survival. A screen of 1120 FDA-approved drugs identified 129 candidates that delayed the dissolution of hypoxia-induced SGs following a return to normoxia. Amongst these candidates, the selective estrogen receptor modulator (SERM) raloxifene delayed SG dissolution in a dose-dependent manner. SG dissolution typically occurs by 15 min post-hypoxia, however pre-treatment of immortalized U251 and U3024 primary GBM cells with raloxifene prevented SG dissolution for up to 2 h. During this raloxifene-induced delay in SG dissolution, translational silencing was sustained, eIF2α remained phosphorylated and mTOR remained inactive. Despite its well-described role as a SERM, raloxifene-mediated delay in SG dissolution was unaffected by co-administration of β-estradiol, nor did β-estradiol alone have any effect on SGs. Importantly, the combination of raloxifene and hypoxia resulted in increased numbers of late apoptotic/necrotic cells. Raloxifene and hypoxia also demonstrated a block in late autophagy similar to the known autophagy inhibitor chloroquine (CQ). Genetic disruption of the SG-nucleating proteins G3BP1 and G3BP2 revealed that G3BP1 is required to sustain the raloxifene-mediated delay in SG dissolution. Together, these findings indicate that modulating the stress response can be used to exploit the hypoxic niche of GBM tumors, causing cell death by disrupting pro-survival stress responses and control of protein synthesis.

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“…These mRNAs ( Ccnd1 and elF2 ) are targeted by miR-124-3p. This molecule is known to regulate global and specific mRNA translation in response to stress-related signals (such as OxS) (65).…”

Section: Discussionmentioning

confidence: 99%

Major Effect of Oxidative Stress on the Male, but Not Female, SP-A1 Type II Cell miRNome

et al. 2019

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Pulmonary surfactant protein A (SP-A) plays an important role in surfactant metabolism and lung innate immunity. In humans there are two proteins, SP-A1 and SP-A2, encoded by SFTPA1 and SFTPA2 , respectively, which are produced by the alveolar type II cells (T2C). We sought to investigate the differential influence of SP-A1 and SP-A2 in T2C miRNome under oxidative stress (OxS). SP-A knock out (KO) and hTG male and female mice expressing SP-A1 or SP-A2 as well as gonadectomized (Gx) mice were exposed to O 3 -induced oxidative stress (OxS) or filtered air (FA). Expression of miRNAs and mRNAs was measured in the T2C of experimental animals. (a) In SP-A1 males after normalizing to KO males, significant changes were observed in the miRNome in terms of sex-OxS effects, with 24 miRNAs being differentially expressed under OxS. (b) The mRNA targets of the dysregulated miRNAs included Ago2, Ddx20, Plcg2, Irs1, Elf2, Jak2, Map2k4, Bcl2, Ccnd1 , and Vhl . We validated the expression levels of these transcripts, and observed that the mRNA levels of all of these targets were unaffected in SP-A1 T2C but six of these were significantly upregulated in the KO (except Bcl2 that was downregulated). (c) Gondadectomy had a major effect on the expression of miRNAs and in three of the mRNA targets ( Irs1, Bcl2 , and Vhl ). Ccnd1 was upregulated in KO regardless of Gx. (d) The targets of the significantly changed miRNAs are involved in several pathways including MAPK signaling pathway, cell cycle, anti-apoptosis, and other. In conclusion, in response to OxS, SP-A1 and male hormones appear to have a major effect in the T2C miRNome.

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Epithelial Cell Transforming 2 and Aurora Kinase B Modulate Formation of Stress Granule–Containing Transcripts from Diverse Cellular Pathways in Astrocytoma Cells

Cited by 9 publications

References 63 publications

Stress Granules Involved in Formation, Progression and Metastasis of Cancer: A Scoping Review

Stress Granules Involved in Formation, Progression and Metastasis of Cancer: A Scoping Review

Raloxifene prevents stress granule dissolution, impairs translational control and promotes cell death during hypoxia in glioblastoma cells

Major Effect of Oxidative Stress on the Male, but Not Female, SP-A1 Type II Cell miRNome

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