Ease-of-use protocol for the rapid detection of third-generation cephalosporin resistance in Enterobacteriaceae isolated from blood cultures using matrix-assisted laser desorption ionization–time-of-flight mass spectrometry

Foschi, Claudio; Compri, Monica; Смирнова, В С; Denicolò, Agnese; Nardini, Paola; Tamburini, Maria Vittoria; Lombardo, Donatella; Landini, M.P.; Ambretti, Simone

doi:10.1016/j.jhin.2016.02.020

Cited by 10 publications

(4 citation statements)

References 8 publications

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“…The molecular peak of CTX ([M+H] + ) was detectable at ~456 Da with the sodium adducts ([M+Na] + ) at ~478 Da, as reported previously using VITEK ® MS Plus [ 14 ] and MALDI Biotyper ® [ 15 ], when incubated for 60 min. The molecular signal of elimination of the acetyl group of CTX was also detectable at ~396 Da as previously reported [ 19 , 20 , 21 , 22 , 23 ]. The MS spectrum for the supernatant of E. coli ATCC 25922, as the β-lactamase-negative strain incubated with CTX, was similar to that for CTX in saline ( Figure 1 a,b).…”

Section: Resultssupporting

confidence: 84%

“…Recently, rapid detection was carried out by directly analyzing positive blood cultures. In this case, the incubation times were set for 1 h [21,23], 1.5 h [20,22], and 3 h [19]. In this study, ESBL-producing E. coli was distinguishable within a culture time of 15 min using Equation (1), where the ratio was calculated by dividing the sum of the hydrolyzed signal intensities by the sum of the signal intensities of those targeted for observation.…”

Section: Discussionmentioning

confidence: 99%

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MALDI-TOF MS Approaches for the Identification of the Susceptibility of Extended-Spectrum β-Lactamases in Escherichia coli

Matsumura

Ikegaya

2023

Microorganisms

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The increase in multidrug-resistant microorganisms that produce extended-spectrum β-lactamases (ESBLs) and carbapenemases is a serious problem worldwide. Recently, matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) has been used for the rapid detection of antibiotic-resistant bacteria. The objective of this study was to establish a method to detect ESBL-producing Escherichia coli by monitoring the hydrolyzation of cefotaxime (CTX) using MALDI-TOF MS. According to the ratio of the peak intensity of CTX and hydrolyzed-CTX-related compounds, the ESBL-producing strains could be clearly distinguished after 15 min of incubation. Moreover, the minimum inhibitory concentration (MIC) values for E. coli were 8 μg/mL and lower than 4 μg/mL, which could be distinguished after 30 min and 60 min of incubation, respectively. The enzymatic activity was determined using the difference in the signal intensity of the hydrolyzed CTX at 370 Da for the ESBL-producing strains incubated with or without clavulanate. The ESBL-producing strains with low enzymatic activity or blaCTX-M genes could be detected by monitoring the hydrolyzed CTX. These results show that this method can rapidly detect high-sensitivity ESBL-producing E. coli.

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Section: Resultssupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

MALDI-TOF MS Approaches for the Identification of the Susceptibility of Extended-Spectrum β-Lactamases in Escherichia coli

Matsumura

Ikegaya

2023

Microorganisms

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“…In China, tigecycline, which was launched for clinical usage in 2010, is the primary choice for treatment of serious XDR bacterial infections. This drug was more preferred than colistin, another last-line antibiotic which was recently approved for clinical application at the end of 2017 but nevertheless exhibits toxicity 4 . With the emergence and global spread of the plasmid-borne colistin-resistance gene mcr-1 in recent years, however, the clinical potential of colistin has been significantly compromised 5 .…”

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Epidemiological and phylogenetic analysis reveals Flavobacteriaceae as potential ancestral source of tigecycline resistance gene tet(X)

et al. 2020

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Emergence of tigecycline-resistance tet(X) gene orthologues rendered tigecycline ineffective as last-resort antibiotic. To understand the potential origin and transmission mechanisms of these genes, we survey the prevalence of tet(X) and its orthologues in 2997 clinical E. coli and K. pneumoniae isolates collected nationwide in China with results showing very low prevalence on these two types of strains, 0.32% and 0%, respectively. Further surveillance of tet(X) orthologues in 3692 different clinical Gram-negative bacterial strains collected during 1994–2019 in hospitals in Zhejiang province, China reveals 106 (2.7%) tet(X)-bearing strains with Flavobacteriaceae being the dominant (97/376, 25.8%) bacteria. In addition, tet(X)s are found to be predominantly located on the chromosomes of Flavobacteriaceae and share similar GC-content as Flavobacteriaceae. It also further evolves into different orthologues and transmits among different species. Data from this work suggest that Flavobacteriaceae could be the potential ancestral source of the tigecycline resistance gene tet(X).

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“…Ease-of-use protocol for the rapid detection of third-generation cephalosporin resistance in Enterobacteriaceae isolated from blood cultures using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry [8] The last decade has seen a technological explosion in diagnostic microbiology laboratories. Slowly the potential benefits of these technologies to IPC are being reported.…”

Section: Faecal Microbiota Transplantation For Recurrentmentioning

confidence: 99%

Ten recent articles from the Journal of Hospital Infection that would not have been seen in Issue 1

Gray

2018

Journal of Hospital Infection

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C. auris was first isolated from the ear of a Japanese patient in 2009. Since then this multidrug-resistant Candida species has becoming a global threat, causing infections that are associated with a high mortality. Outbreaks of infection occur mainly in ICU settings, and are difficult to control. There is still much to learn about the epidemiology and control of this pathogen. An android app for recording hand hygiene observation data [2]* Tel.: þ44 (0)207 125 0822.

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Ease-of-use protocol for the rapid detection of third-generation cephalosporin resistance in Enterobacteriaceae isolated from blood cultures using matrix-assisted laser desorption ionization–time-of-flight mass spectrometry

Cited by 10 publications

References 8 publications

MALDI-TOF MS Approaches for the Identification of the Susceptibility of Extended-Spectrum β-Lactamases in Escherichia coli

MALDI-TOF MS Approaches for the Identification of the Susceptibility of Extended-Spectrum β-Lactamases in Escherichia coli

Epidemiological and phylogenetic analysis reveals Flavobacteriaceae as potential ancestral source of tigecycline resistance gene tet(X)

Ten recent articles from the Journal of Hospital Infection that would not have been seen in Issue 1

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