Comparison of Citrate-Fluoride-EDTA with Fluoride-EDTA Additives to Stabilize Plasma Glucose Measurements in Women Being Screened during Pregnancy with an Oral Glucose Tolerance Test: A Prospective Observational Study

Daly, Niamh; Flynn, Iseult; Carroll, Ciara; Stapleton, Mary; O’Kelly, Ruth; Turner, Michael J.

doi:10.1373/clinchem.2016.254466

Cited by 15 publications

(8 citation statements)

References 5 publications

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“…Various other methods have also been utilized to obtain the most accurate glucose levels. Studies comparing citrate and fluoride containing samples showed that samples containing citrate produced higher diagnostic accuracy of glucose levels, although some recent tube preparations have a combination of these (76)(77)(78)(79). Also, studies have shown that utilizing various tubes for glucose concentrations do not produce interchangeable glucose values (80).…”

Section: Diagnostic Considerationsmentioning

confidence: 99%

Approach to hypoglycemia in infants and children

Gandhi

2017

Transl. Pediatr

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Hypoglycemia is a heterogeneous disorder with many different possible etiologies, including hyperinsulinism, glycogen storage disorders, fatty acid disorders, hormonal deficiencies, and metabolic defects, among others. This condition affects newborns to adolescents, with various approaches to diagnosis and management. This paper will review current literature on the history of hypoglycemia, current discussion on the definition of hypoglycemia, as well as etiologies, diagnosis, and management.

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Section: Diagnostic Considerationsmentioning

confidence: 99%

Approach to hypoglycemia in infants and children

Gandhi

2017

Transl. Pediatr

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“…A possible alternative is the use of fluoridecitrate preservative for glucose analysis. 10,11 The recommendation of citrate as an antiglycolytic agent has been popularized recently, due its reemergence as a viable glycolytic inhibitor. 6,10,12,13 Sample tubes containing either powdered or liquid preservative have been studied, and the results are compared against the traditional fluoride glucose tubes.…”

Section: Discussionmentioning

confidence: 99%

Time and temperature affect glycolysis in blood samples regardless of fluoride-based preservatives: a potential underestimation of diabetes

et al. 2017

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Background The inhibition of glycolysis prior to glucose measurement is an important consideration when interpreting glucose tolerance tests. This is particularly important in gestational diabetes mellitus where prompt diagnosis and treatment is essential. A study was planned to investigate the effect of preservatives and temperature on glycolysis. Methods Blood samples for glucose were obtained from consented females. Lithium heparin and fluoride-EDTA samples transported rapidly in ice slurry to the laboratory were analysed for glucose concentration and then held either in ice slurry or at room temperature for varying time intervals. Paired fluoride-citrate samples were received at room temperature and held at room temperature, with analysis at similar time intervals. Results No significant difference was noted between mean glucose concentrations when comparing different sample types received in ice slurry. The mean glucose concentrations decreased significantly for both sets of samples when held at room temperature (0.4 mmol/L) and in ice slurry (0.2 mmol/L). A review of patient glucose tolerance tests reported in our hospital indicated that 17.8% exceeded the recommended diagnostic criteria for gestational diabetes mellitus. It was predicted that if the results of fasting samples were revised to reflect the effect of glycolysis at room temperature, the adjusted diagnostic rate could increase to 35.3%. Conclusion Preanalytical handling of blood samples for glucose analysis is vital. Fluoride-EDTA is an imperfect antiglycolytic, even when the samples are transported and analysed rapidly provides such optimal conditions. The use of fluoride-citrate tubes may offer a viable alternative in the diagnosis of diabetes mellitus.

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“…A significant proportion of women in mid-pregnancy to late pregnancy have plasma glucose values above GDM diagnostic thresholds; also their distribution of glucose, especially fasting glucose, is narrow (1)(2)(3). In this setting, a small bias in measured glucose can have large diagnostic consequences.…”

Section: Will the Use Of Lyophilized Citrate Tubes Lead To The Over-dmentioning

confidence: 99%

“…Some laboratories therefore recommend using collection tubes containing citrate stabi-lizer, including for the diagnosis of GDM (3). In nonpregnant populations, citrated tubes may show a small positive bias (4,5).…”

Section: Will the Use Of Lyophilized Citrate Tubes Lead To The Over-dmentioning

confidence: 99%

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Will the Use of Lyophilized Citrate Tubes Lead to the Over-Diagnosis of Diabetes in Pregnancy?

Carey

Churcher

Hughes

et al. 2017

The Journal of Applied Laboratory Medicine

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COOH losses in urine from contact with LDPE.Because of limitations of available leftover patient urine volume, analysis time, and cost, we only performed singlicate experiments, which demonstrated that the THC-COOH concentrations in the inverted, metal lid containers of urine specimens varied by −50% to +9% from their plastic-only counterparts. When averaged, a reduction of 29% was observed. Specimens collected and stored in these containers could potentially yield falsely low THC-COOH results. We caution the adoption of new specimen containers until they are investigated on all subjected analytes.

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Comparison of Citrate-Fluoride-EDTA with Fluoride-EDTA Additives to Stabilize Plasma Glucose Measurements in Women Being Screened during Pregnancy with an Oral Glucose Tolerance Test: A Prospective Observational Study

Cited by 15 publications

References 5 publications

Approach to hypoglycemia in infants and children

Approach to hypoglycemia in infants and children

Time and temperature affect glycolysis in blood samples regardless of fluoride-based preservatives: a potential underestimation of diabetes

Will the Use of Lyophilized Citrate Tubes Lead to the Over-Diagnosis of Diabetes in Pregnancy?

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