The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae)

Jia, H. Y.; Li, Geng; Li, Yunhe; Wang, Qiang; Diao, Qingyun; Zhou, Ting; Dai, Pingli

doi:10.1038/srep24664

Cited by 56 publications

(50 citation statements)

References 48 publications

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“…These taxa are consistent with those found in a recent study [15,16,[20][21][22][23]. These studies suggest that the dominant groups of bacteria in honeybee guts may not be easily changed by external factors, including season, temperature, sugar types, and even toxins [22]. However, in the current experiment, the relative abundance of the dominant microbiota changed with the different sugar types and seasons.…”

Section: Discussionsupporting

confidence: 92%

“…The dominant microbiota established in the guts of honeybees were Proteobacteria, Firmicutes (Lactobacillus), Actinobacteria (Bifidobacterium), and Bacteroidetes. These taxa are consistent with those found in a recent study [15,16,[20][21][22][23]. These studies suggest that the dominant groups of bacteria in honeybee guts may not be easily changed by external factors, including season, temperature, sugar types, and even toxins [22].…”

Section: Discussionsupporting

confidence: 91%

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The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering

Wang¹,

Liu

Wang³

et al. 2019

Preprint

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Background : The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees. Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood. Therefore, in this study, the influence of different sugar diets on honeybee gut bacteria during overwintering was examined.Methods: The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V4 region of 16S rDNA using the Illumina MiSeq platform. Results: The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus , 15.91%), Actinobacteria (4.06%; Bifidobacterium , 3.34%), and Bacteroidetes (1.72%). The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.However, the relative abundance of the dominant taxa was affected by those factors. In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group. Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae ) and Alphaproteobacteria (Rhizobiales and Mitochondria ) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut. High-fructose syrup increased the relative abundance of Betaproteobacteria (Neisseriales: Neisseriaceae ) of the midgut. Conclusion: The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering. The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering

Wang¹,

Liu

Wang³

et al. 2019

Preprint

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show abstract

“…So far, the non-target insect assessment concerning Cry1Ie has only focused on the survival of Chinese honey bees, Aphis cerana cerana (Hymenoptera: Apidae) [39], the diversity of the midgut bacteria of the worker bees, Apis mellifera ligustica (Hymenoptera: Apidae) [40], and the survival, pollen consumption, and olfactory learning of young adult honey bees, A. mellifera [41]. None of those studies has revealed any adverse effects of Cry1Ie consumption.…”

Section: Discussionmentioning

confidence: 99%

Consumption of Bt Maize Pollen Containing Cry1Ie Does Not Negatively Affect Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)

Liu

et al. 2017

Toxins

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Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) are prevalent predators and pollen feeders in East Asian maize fields. They are therefore indirectly (via prey) and directly (via pollen) exposed to Cry proteins within Bt-transgenic maize fields. The effects of Cry1Ie-producing transgenic maize pollen on the fitness of P. japonica was assessed using two dietary-exposure experiments in the laboratory. In the first experiment, survival, larval developmental time, adult fresh weight, and fecundity did not differ between ladybirds consuming Bt or non-Bt maize pollen. In the second experiment, none of the tested lethal and sublethal parameters of P. japonica were negatively affected when fed a rapeseed pollen-based diet containing Cry1Ie protein at 200 μg/g dry weight of diet. In contrast, the larval developmental time, adult fresh weight, and fecundity of P. japonica were significantly adversely affected when fed diet containing the positive control compound E-64. In both experiments, the bioactivity of the Cry1Ie protein in the food sources was confirmed by bioassays with a Cry1Ie-sensitive lepidopteran species. These results indicated that P. japonica are not affected by the consumption of Cry1Ie-expressing maize pollen and are not sensitive to the Cry1Ie protein, suggesting that the growing of Bt maize expressing Cry1Ie protein will pose a negligible risk to P. japonica.

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“…These pools of amplicons were sequenced using an Illumina HiSeq2500 platform. The sequencing data sets were cleaned and trimmed according to Jia et al (2016) and processed with Qiime (Caporaso et al, 2010) using Uparse with a 97% stringency to yield operational taxonomic units (OTUs). OTUs were taxonomically classified using the Mothur classifier (Wang, Garrity, Tiedje, & Cole, 2007) with 0.8 confidence interval against the SILVA database 123 release of July 2015.…”

Section: Methodsmentioning

confidence: 99%

Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures

Rombouts

Kranendonk

Regueira

et al. 2020

Biotech & Bioengineering

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Lactic acid‐producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology‐based process design and can aid the development of sustainable and energy‐efficient bioprocesses. Here we demonstrate the enrichment of lactic acid bacteria in a controlled sequencing batch bioreactor environment using a glucose‐based medium supplemented with peptides and B vitamins. A mineral medium enrichment operated in parallel was dominated by Ethanoligenens species and fermented glucose to acetate, butyrate and hydrogen. The complex medium enrichment was populated by Lactococcus, Lactobacillus and Megasphaera species and showed a product spectrum of acetate, ethanol, propionate, butyrate and valerate. An intermediate peak of lactate was observed, showing the simultaneous production and consumption of lactate, which is of concern for lactic acid production purposes. This study underlines that the competitive advantage for lactic acid‐producing bacteria primarily lies in their ability to attain a high biomass specific uptake rate of glucose, which was two times higher for the complex medium enrichment when compared to the mineral medium enrichment. The competitive advantage of lactic acid production in rich media can be explained using a resource allocation theory for microbial growth processes.

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The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae)

Cited by 56 publications

References 48 publications

The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering

The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering

Consumption of Bt Maize Pollen Containing Cry1Ie Does Not Negatively Affect Propylea japonica (Thunberg) (Coleoptera: Coccinellidae)

Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures

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