Highly active antibody-modified magnetic polyelectrolyte capsules

Valdepérez, Daniel; Pino, Pablo del; Sánchez, Lourdes; Parak, Wolfgang J.; Pelaz, Beatriz

doi:10.1016/j.jcis.2016.04.003

Cited by 24 publications

(21 citation statements)

References 44 publications

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“…An additional strategy adopted for mAb nanoconjugation comprised the oxidation of a saccharide residue (carbohydratecontaining cis-diol groups) present in the Fc portion of the antibody. 5,53 This procedure comprised the formation of an aldehyde group capable of reacting with nucleophilic functionalities (such as amines) that are appropriately visible on the NP surfaces, as shown in Fig. S1.…”

Section: Resultsmentioning

confidence: 99%

Does conjugation strategy matter? Cetuximab-conjugated gold nanocages for targeting triple-negative breast cancer cells

et al. 2019

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Section: Resultsmentioning

confidence: 99%

Does conjugation strategy matter? Cetuximab-conjugated gold nanocages for targeting triple-negative breast cancer cells

et al. 2019

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“…Because NPs and microparticles with empty cores are promising for targeted drug delivery into regions of the primary tumor and metastasis , we tested whether 2ICG + GNR MNCs can serve as advanced dual PA and fluorescence contrast agents. For this purpose, we used mice and two‐color PAFC (671 and 820 nm) integrated with fluorescence FC (PAFFC) in vivo, operated in the near‐infrared region.…”

Section: Resultsmentioning

confidence: 99%

“…MNCs were also characterized by multimodal PA techniques described before . Briefly, a PA microscope‐spectroscope module was built on the technical platform of an Olympus IX81 inverted microscope by using a tunable OPO (Opolette HR 355 LD, Opotek, Inc., Carlsbad, California) with the following parameters: tunable spectral range, 420‐2200 nm; pulse width, 5 ns; pulse repetition rate, 100 Hz; and fluence range, 0.1‐10 4 mJ/cm 2 .…”

Section: Methodsmentioning

confidence: 99%

“…Integrating these discoveries with the recent developments of advanced nanocomposite structures, used before mostly in drug delivery , affords new PA‐ and PT‐active structures with controllable fluorescence. Fluorescent modality can be used to verify the results of PA measurements and to examine biodistribution of novel contrast agents in vivo .…”

Section: Introductionmentioning

confidence: 99%

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Photoacoustic and fluorescent effects in multilayer plasmon‐dye interfaces

Новоселова

Bratashov

Sarimollaoglu

et al. 2019

Journal of Biophotonics

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Progress in understanding the cell biology and diseases depends on advanced imaging and labeling techniques. Here, we address this demand by exploring novel multilayered nanocomposites (MNCs) with plasmonic nanoparticles and absorbing dyes in thin nonabsorbing shells as supercontrast multimodal photoacoustic (PA) and fluorescent agents in the near-infrared range. The proof of concept was performed with gold nanorods (GNRs) and indocyanine green (ICG) dispersed in a matrix of biodegradable polymers. We demonstrated synergetic PA effects in MNCs with the gold-ICG interface that could not be achieved with ICG and GNRs alone. We also observed ultrasharp PA and emission peaks that could be associated with nonlinear PA and spaser effects, respectively. Low-toxicity multimodal MNCs with unique plasmonic, thermal and acoustic properties have the potential to make a breakthrough in PA flow cytometry and near-infrared spasers in vivo by using the synergetic interaction of plasmonic modes with a nearby absorbing medium. K E Y W O R D S biocompatible polymers, fluorescence, fluorescence quenching, gold nanorods, in vivo flow cytometry, indocyanine green, multilayer composite, photoacoustic effect

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“…The versatility of LbL systems is also determined by a wide range of their modification possibilities that enable additional functionalities, such as light or magnetic field sensitivity, targeting abilities, catalytic or bioimaging properties, and others [26,27]. Similar to genetic material, organic or inorganic nanomaterials can be either incorporated between layers of polymers during the LbL procedure [28], or attached onto the surface of LbL particles [29], or loaded into the cavity of the LbL systems [30][31][32] (Figure 2(a)).…”

Section: Inorganic and Organic Nanostructures In Lbl Systems For Controlled Triggering And Manipulationmentioning

confidence: 99%

Layer-by-Layer technique as a versatile tool for gene delivery applications

Linnik

Tarakanchikova

Zyuzin

et al. 2021

Expert Opinion on Drug Delivery

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Introduction: Gene therapy is a breakthrough medical field which focuses on the therapeutic delivery of recombinant nucleic acids in order to treat or prevent a broad spectrum of diseases. However, a number of important obstacles remain before its wide introduction into clinical practice can be envisaged. One of the biggest bottlenecks is the lack of efficient and safe delivery technologies, particularly, for in vivo distribution. Above and beyond standard requirements for carriers, the delivery systems for gene therapy ideally use a hit-and-run principle (to minimize off-target effect and display of immunogenic moieties). None of the currently used viral vectors fulfills all of these requirements. Therefore, the growing variety of non-viral delivery platforms represents a promising alternative.Areas covered: This review summarizes the Layer-by-Layer (LbL) approaches that can be effectively used for the gene delivery, considering various examples with the transfer of pDNA, mRNA, siRNA as well as genome-editing tools. Ex vivo gene modification of clinically relevant cells and clinical aspects for possible application of LbL systems in gene therapy are also underlined. Expert opinion:The LbL technique provides broad opportunities for the delivery of genetic material for various purposes and offers promise for future clinical application in gene therapy.

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Highly active antibody-modified magnetic polyelectrolyte capsules

Cited by 24 publications

References 44 publications

Does conjugation strategy matter? Cetuximab-conjugated gold nanocages for targeting triple-negative breast cancer cells

Does conjugation strategy matter? Cetuximab-conjugated gold nanocages for targeting triple-negative breast cancer cells

Photoacoustic and fluorescent effects in multilayer plasmon‐dye interfaces

Layer-by-Layer technique as a versatile tool for gene delivery applications

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