Biomimetic hydrogel loaded with silk and l‐proline for tissue engineering and wound healing applications

Abstract

The aim of this article was to develop silk protein (SF) and l-proline (LP) loaded chitosan-(CS) based hydrogels via physical cross linking for tissue engineering and wound healing applications. Silk fibroin, a biodegradable and biocompatible protein, and l-proline, an important imino acid that is required for collagen synthesis, were added to chitosan to improve the wound healing properties of the hydrogel. Characterization of these hydrogels revealed that CS/SF/LP hydrogels were blended properly and LP incorporated hydrogels showed excellent thermal stability and good surface morphology. Swelling study showed the water holding efficiency of the hydrogels to provide enough moisture at the wound surface. In vitro biodegradation results demonstrated that the hydrogels had good degradation rate in PBS with lysozyme. LP loaded hydrogels showed approximately a twofold increase in antioxidant activity. In vitro cytocompatibility studies using NIH 3T3 L1 cells showed increased cell viability (p < 0.01), migration, proliferation and wound healing activity (p < 0.001) in LP loaded hydrogels compared to CS and CS/SF hydrogels. Cell adhesion on SF and LP hydrogels were observed using SEM and compared to CS hydrogel. LP incorporation showed 74-78% of wound closure compared to 35% for CS/SF and 3% for CS hydrogels at 48 h. These results suggest that incorporation of LP can significantly accelerate wound healing process compared to pure CS and SF-loaded CS hydrogels. Hence, CS/LP hydrogels could be a potential wound dressing material for the enhanced wound tissue regeneration and repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1401-1408, 2017.

Reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications

“…Swelling kinetics was studied to determine the degree of swelling of the scaffolds at equilibrium [ 41 , 42 ]. Briefly, scaffolds, cut into smaller pieces of same weight and roughly the same volume, were immersed in PBS.…”

Morin incorporated polysaccharide–protein (psyllium–keratin) hydrogel scaffolds accelerate diabetic wound healing in Wistar rats

“…The cell attachment in scaffolds was examined using scanning electron microscopy (SEM) and images have been taken. 39 In vivo studies Animal grouping and maintenance. Healthy male Wistar albino rats with body mass greater than 200 g were selected for demonstration of diabetic wound healing in vivo.…”

Advances and Impact of Antioxidant Hydrogel in Chronic Wound Healing

“…Therefore, maintaining the balance of redox in cells, that is, to effectively achieve antioxidant, can hopefully avoid abnormal cell growth and disordered immune response. As expected, it has been proved that antioxidation can accelerate wound healing, especially for chronic wound repair . Therefore, the introduction of antioxidant function has become an effective way to accelerate the repair of chronic wounds.…”

“…As expected, it has been proved that antioxidation can accelerate wound healing, especially for chronic wound repair. [16,17] Therefore, the introduction of antioxidant function has become an effective way to accelerate the repair of chronic wounds.…”