Effects of Low-Level Laser Therapy Applied Before Treadmill Training on Recovery of Injured Skeletal Muscle in Wistar Rats

Adabbo, Mayna; Paolillo, Fernanda Rossi; Bossini, Paulo Sérgio; Rodrigues, Natália Camargo; Bagnato, Vanderlei Salvador; Parizotto, Nivaldo Antônio

doi:10.1089/pho.2015.4031

Cited by 15 publications

(15 citation statements)

References 43 publications

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“…Next, we analyzed the cross-sectional area of the myofibers in the injured area after the treatment trial. A statistically significant increase in myofiber cross-sectional area was found in rats in the Cells and Cells+Exercise groups compared with the Vehicle group (247.5 ± 10.7 µm 2 ; P < .001) (Figure 4, A and B). Moreover, the Cells+Exercise group showed the largest increase in size of myofibers (686.0 ± 11.6 µm 2 ), which was significantly higher than that in the Cells group (612.3 ± 21.4 µm 2 ; P = .001) demonstrating a synergistic effect of cell therapy and exercise in the treatment of injured skeletal muscle.…”

Section: Resultsmentioning

confidence: 90%

Muscle Precursor Cells Enhance Functional Muscle Recovery and Show Synergistic Effects With Postinjury Treadmill Exercise in a Muscle Injury Model in Rats

et al. 2021

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Background: Skeletal muscle injuries represent a major concern in sports medicine. Cell therapy has emerged as a promising therapeutic strategy for muscle injuries, although the preclinical data are still inconclusive and the potential clinical use of cell therapy has not yet been established. Purpose: To evaluate the effects of muscle precursor cells (MPCs) on muscle healing in a small animal model. Study Design: Controlled laboratory study. Methods: A total of 27 rats were used in the study. MPCs were isolated from rat (n = 3) medial gastrocnemius muscles and expanded in primary culture. Skeletal muscle injury was induced in 24 rats, and the animals were assigned to 3 groups. At 36 hours after injury, animals received treatment based on a single ultrasound-guided MPC (105 cells) injection (Cells group) or MPC injection in combination with 2 weeks of daily exercise training (Cells+Exercise group). Animals receiving intramuscular vehicle injection were used as controls (Vehicle group). Muscle force was determined 2 weeks after muscle injury, and muscles were collected for histological and immunofluorescence evaluation. Results: Red fluorescence–labeled MPCs were successfully transplanted in the site of the injury by ultrasound-guided injection and were localized in the injured area after 2 weeks. Transplanted MPCs participated in the formation of regenerating muscle fibers as corroborated by the co-localization of red fluorescence with developmental myosin heavy chain (dMHC)–positive myofibers by immunofluorescence analysis. A strong beneficial effect on muscle force recovery was detected in the Cells and Cells+Exercise groups (102.6% ± 4.0% and 101.5% ± 8.5% of maximum tetanus force of the injured vs healthy contralateral muscle, respectively) compared with the Vehicle group (78.2% ± 5.1%). Both Cells and Cells+Exercise treatments stimulated the growth of newly formed regenerating muscles fibers, as determined by the increase in myofiber cross-sectional area (612.3 ± 21.4 µm2 and 686.0 ± 11.6 µm2, respectively) compared with the Vehicle group (247.5 ± 10.7 µm2), which was accompanied by a significant reduction of intramuscular fibrosis in Cells and Cells+Exercise treated animals (24.2% ± 1.3% and 26.0% ± 1.9% of collagen type I deposition, respectively) with respect to control animals (40.9% ± 4.1% in the Vehicle group). MPC treatment induced a robust acceleration of the muscle healing process as demonstrated by the decreased number of dMHC-positive regenerating myofibers (enhanced replacement of developmental myosin isoform by mature myosin isoforms) (4.3% ± 2.6% and 4.1% ± 1.5% in the Cells and Cells+Exercise groups, respectively) compared with the Vehicle group (14.8% ± 13.9%). Conclusion: Single intramuscular administration of MPCs improved histological outcome and force recovery of the injured skeletal muscle in a rat injury model that imitates sports-related muscle injuries. Cell therapy showed a synergistic effect when combined with an early active rehabilitation protocol in rats, which suggests that a combination of treatments can generate novel therapeutic strategies for the treatment of human skeletal muscle injuries. Clinical Relevance: Our study demonstrates the strong beneficial effect of MPC transplant and the synergistic effect when the cell therapy is combined with an early active rehabilitation protocol for muscle recovery in rats; this finding opens new avenues for the development of effective therapeutic strategies for muscle healing and clinical trials in athletes undergoing MPC transplant and rehabilitation protocols.

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Section: Resultsmentioning

confidence: 90%

Muscle Precursor Cells Enhance Functional Muscle Recovery and Show Synergistic Effects With Postinjury Treadmill Exercise in a Muscle Injury Model in Rats

et al. 2021

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“…Techniques used separately may have slower therapeutic responses, and therefore the synergistic use of vacuum therapy with laser therapy may enhance the quality and speed of the biological response in the rehabilitation process. Several studies1, 2, 7, 18 ) showed that mechanical stimulus such as therapeutic ultrasound1, 2 ) and physical exercise7, 18 ) were associated with laser therapy and showed significant synergistic effects on physical rehabilitation, including pain relief, accelerated tissue repair and increased physical function. Vacuum therapy is also a mechanical stimulus to potentiate laser therapy, leading to synergistic effects.…”

Section: Discussionmentioning

confidence: 99%

“…Laser is an acronym for Light Amplification by Stimulated Emission of Radiation. Laser therapy advanced significantly in the mid-1960s and to date has been used in the health area, such as medicine, dentistry and physiotherapy producing satisfactory results in treatments that require, for example, anti-inflammatory and analgesic action, as well as acceleration of tissue healing processes5,6,7 ) . Penetration of skin by light depends on the incident wavelength and optical properties of the biological tissues.…”

Section: Introductionmentioning

confidence: 99%

Synergistic effects of vacuum therapy and laser therapy on physical rehabilitation

et al. 2019

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[Purpose] This study aims to evaluate the synergistic effects of laser therapy and vacuum therapy in the rehabilitation of some disorders of the osteomuscular articular system (shoulder bursitis, low back pain, hip dysfunction after long-term impact of femoral endoprosthesis). [Participants and Methods] Three case reports were described. A vacuum laser system was used, which is a device that contains 1 handpiece with 3 red lasers (660 nm) and 3 infrared lasers (808 nm). This device contains 6 laser beams that are arranged around the vacuum chamber bore which generate negative pressure. Two weekly sessions were held, totalling 10 treatment sessions. Active goniometry was performed to assess range of motion (ROM) of the shoulder, lumbar spine and hip, and visual analogue scale (VAS) was used to evaluate pain in the pre and posttreatment period. [Results] There was a pain reduction and an increase in ROM in the different clinical cases. [Conclusion] Potentiating laser therapy through suctioning during the rehabilitation process leads to pain relief and increased functionality.

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“…Previous studies have demonstrated the potential value of combining infrared radiation with treadmill training to further enhance muscle function in an animal model (Adabbo et al 2016) as well as clinical trial (Paolillo et al 2011). However, the potential long-term effects of infrared radiation and treadmill training on bone mass, to our knowledge, have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Effects of infrared radiation and exercise on bone mass: implications for the prevention and management of osteoporosis

et al. 2020

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The aim of the current study was to investigate the effects of infrared radiation in conjunction with treadmill training on bone mass and muscle performance. Methods Twenty postmenopausal women aged 50 to 60 years participated in this study. The investigated groups were (i) the Light Emitting Diodes (LED) group, which performed treadmill training associated with photobiomodulation therapy (n = 10) and (ii) the exercise group, which carried out treadmill training only (n = 10). Eighteen women completed the full study (9 per group). Training was performed for 12 months, twice a week for 45 min per session at 85 to 90% of maximal heart rate (HR max); training intensity was determined by a progressive exercise testing. The irradiation parameters were infrared-LEDs (850 nm wavelength) at 100 mW power, 39 mW/cm 2 power density, and 108 J/cm 2 fluence for 45 min. Isokinetic dynamometry and bone densitometry were performed. Results The reduction in total femur bone mineral density (BMD) was significantly higher (p = 0.04) for the exercise group (Δ = − 0.024 ± 1.055 mg/cm 3) compared with the LED group (Δ = − 0.011 ± 0.013 mg/cm 3). The number of muscle contractions was significantly increased, while the fatigue was significantly reduced only in the LED group compared with baseline (p < 0.05). The average power was significantly higher for the LED and exercise groups compared with baseline (p < 0.01). There was no significant difference in quadriceps performance between 6 and 12 months for either group (p ≥ 0.05). Conclusion Infrared radiation associated with treadmill training may synergistically act to further enhance muscle performance and attenuate the reduction of femur BMD, preventing osteoporosis and its associated health consequences.

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Effects of Low-Level Laser Therapy Applied Before Treadmill Training on Recovery of Injured Skeletal Muscle in Wistar Rats

Cited by 15 publications

References 43 publications

Muscle Precursor Cells Enhance Functional Muscle Recovery and Show Synergistic Effects With Postinjury Treadmill Exercise in a Muscle Injury Model in Rats

Muscle Precursor Cells Enhance Functional Muscle Recovery and Show Synergistic Effects With Postinjury Treadmill Exercise in a Muscle Injury Model in Rats

Synergistic effects of vacuum therapy and laser therapy on physical rehabilitation

Effects of infrared radiation and exercise on bone mass: implications for the prevention and management of osteoporosis

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