Small molecule detection by lateral flow strips via aptamer-gated silica nanoprobes

Abstract

A fast, sensitive and ratiometric biosensor strategy for small molecule detection was developed through nanopore actuation. The new platform engineers together, a highly selective molecular recognition element, aptamers, and a novel signal amplification mechanism, gated nanopores. As a proof of concept, aptamer gated silica nanoparticles have been successfully used as a sensing platform for the detection of ATP concentrations at a wide linear range from 100 μM up to 2 mM.

Simple Endotoxin Detection Using Polymyxin‐B‐Gated Nanoparticles

“…However, in the presence of the analyte, the pores open due to the interaction of the analyte with the capping ensemble leading to cargo release. [21][22][23][24][25][26] One of the advantages of these solids is the potential existence of amplification features as few analyte molecules may trigger gate opening, and induce the release of a high amount of entrapped dye molecules. Given our interest in exploring the potential use of gated nanomaterials in sensing protocols, we report herein a new nanodevice based on mesoporous silica nanoparticles (MSNs) functionalized with carboxylate moieties and capped with the cationic peptide polymyxin B for the selective and sensitive detection of endotoxin.…”

Toward Label‐Free Optical Multiplexing of Analytes in Indicator Release Lateral Flow Assays via Detection Zones Containing Tailored Capture Materials

“…Özalp's group has recently reported on a gAID-type LFA in which the loaded materials are arranged in lines and the analytical response is assessed as a signal decrease in those lines as a consequence of indicator release. [17] While this approach is certainly appealing, it is an indirect detection scheme, not taking full advantage of measuring the released indicator in a focused area. In the present work, we thus explored the possibilities of creating dedicated capture zones directly on/in the strips for the improved detection of released indicators, relying on electrostatic attraction of ionic polymers and functionalized mesoporous silicas as well as the recognition abilities of molecularly imprinted polymers.…”

Small molecule detection with aptamer based lateral flow assays: Applying aptamer-C-reactive protein cross-recognition for ampicillin detection

“…Few reports on the detection of LMW compounds using aptamer based LFAs, however, have been published. To the best of our knowledge, the reports from Zhang 10 , Özalp 11 , Fischer 12 , Liu 13 , Mazumdar 14 , Wang 15 and Shim 16 are the only reported aptamer based LFA systems for detection of small molecules up to date, more precisely, for detection of adenosine triphosphate (ATP), p -aminohippuric acid, adenosine, cocaine, lead, Ochratoxin A, Aflatoxin B1, E2 and BPA. Problems of aptamer selection are, however, inversely correlated to the size of the target molecule and its associated interaction sites 17 .…”