2016
DOI: 10.1073/pnas.1522958113
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Comprehensive mutagenesis of thefimSpromoter regulatory switch reveals novel regulation of type 1 pili in uropathogenicEscherichia coli

Abstract: Type 1 pili (T1P) are major virulence factors for uropathogenic Escherichia coli (UPEC), which cause both acute and recurrent urinary tract infections. T1P expression therefore is of direct relevance for disease. T1P are phase variable (both piliated and nonpiliated bacteria exist in a clonal population) and are controlled by an invertible DNA switch (fimS), which contains the promoter for the fim operon encoding T1P. Inversion of fimS is stochastic but may be biased by environmental conditions and other signa… Show more

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Cited by 10 publications
(8 citation statements)
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“…Additional cellular factors or cis -acting elements might also participate in the DNA inversion process as was recently demonstrated for the fim switch in uropathogenic E . coli [ 69 ].…”
Section: Discussionmentioning
confidence: 99%
“…Additional cellular factors or cis -acting elements might also participate in the DNA inversion process as was recently demonstrated for the fim switch in uropathogenic E . coli [ 69 ].…”
Section: Discussionmentioning
confidence: 99%
“…Microarray-synthesized promoter libraries and measurement of expression from barcoded transcripts using RNA-Seq instead of flow cytometry can be used to allow multiple loci to be studied simultaneously ( 14 , 18 ). Landing pad technologies for chromosomal integration ( 58 60 ) should enable massively parallel reporter assays to be performed in chromosomes instead of on plasmids. Techniques that combine these assays with transcription start site readout ( 61 ) may provide additional resolution, further allowing the molecular regulators of overlapping RNAP binding sites to be deconvolved or the contributions from separate RNAP binding sites, like those observed on the dgoR promoter, to be better distinguished.…”
Section: Discussionmentioning
confidence: 99%
“…Positive verification of a new inversion locus is relatively straightforward once the locus is known, and two recent studies have used whole-genome sequencing (with Illumina and PacBio data) to achieve accurate quantification of fimS inversion percentages under different conditions (42, 43). However, to truly establish the specificity of the fim recombinases, a strong negative predictive value is required when analyzing whole-genome sequencing data (alternatively, a low noise level).…”
Section: Discussionmentioning
confidence: 99%