Antithrombotic and Antioxidant Activity of Amaranth Hydrolysate Obtained by Activation of an Endogenous Protease

Sabbione, Ana Clara; Ibañez, Sabrina M.; Martínez, E. Nora; Añón, Marı́a Cristina; Scilingo, Adriana Alicia

doi:10.1007/s11130-016-0540-y

Cited by 28 publications

(13 citation statements)

References 35 publications

(45 reference statements)

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“…The SDS profile showed well-defined bands with molecular weights between 57-67 kDa (Figure 1). Silva-Sánchez et al (2008), Tiengo et al (2009), Barrio & Añón (2010), Sabbione et al (2015) and Sabbione et al, (2016) revealed similar results for amaranth isolates of different cultivars.…”

Section: Resultssupporting

confidence: 61%

In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates

et al. 2020

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Section: Resultssupporting

confidence: 61%

In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates

et al. 2020

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“…Seeds and nuts have also shown to contain peptides with good antioxidant activities. Among seeds, rapeseed ( Alashi et al., 2014 ; Cumby et al., 2008 ; He et al., 2012 , 2013 ; Pan et al., 2011 ; Xue et al., 2009a , Xue et al., 2009b ; Yu et al., 2013 ; Zhang et al., 2008 , 2009 ), hemp seed ( Girgih et al., 2014 ; Girgih et al., 2013 , 2014 ; Lu et al., 2010 ), flaxseed ( Karamać et al., 2016 ; Silva et al., 2017 ), sesame ( Das et al., 2012 ; Lu et al., 2019 ; Wang et al., 2012 ), palm ( Ng et al., 2013 ; Zarei et al., 2012 , 2014 ) and amaranth seeds ( Orsini Delgado et al., 2011 ; Sabbione et al., 2016 ) are the most reported. Chia ( Silveira Coelho et al., 2019 ), pumpkin ( Venuste et al., 2013 ), watermelon ( Wen et al., 2019 ), quinoa ( Ren et al., 2017 ), perilla ( Yang et al., 2018 ), moringa ( Aderinola et al., 2019 ), sunflower ( Megías et al., 2008 ) and cassia ( Chai et al., 2019 ) have been also explored.…”

Section: Production and Identification Of Antioxidant Bioactive Peptides From Plant Biomassmentioning

confidence: 99%

“… Not identified. Sabbione et al. (2016) African Yam Bean Seed ( Sphenostylis stenocarpa ) African yam bean seed proteins Enzymatic (Alcalase) Hydrolysates and 4 fractions (UF): MW < 1 kDa, 1 kDa < MW < 3 kDa, 3 kDa < MW < 5 kDa, and 5 kDa < MW < 10 kDa.…”

Section: Production and Identification Of Antioxidant Bioactive Peptides From Plant Biomassmentioning

confidence: 99%

“…ORAC (IC50 = 0.058 ± 0.027 mg/mL TE) and ABTS (IC50 = 2.1 ± 0.3 mg/mL TE). Sabbione et al. (2016) In vitro (solution and linoleic acid model system) DPPH, SRSA, HRSA, FRAP, and linoleic acid oxidation (PV) Fe Small peptide size (MW < 1 kDa) and high level of hydrophobicity seemed to be important for DPPH, SRSA, HSRA and FRAP.…”

Section: Focus On the Antioxidant Activities Of Bioactive Peptides Obtained From Plant Biomassmentioning

confidence: 99%

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Production and antioxidant capacity of bioactive peptides from plant biomass to counteract lipid oxidation

Durand

Beaubier

Ilic

et al. 2021

Current Research in Food Science

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Preventing lipid oxidation, especially with the polyunsaturated fat-based products, is a major concern in sectors as agri-food and cosmetic. Even though the efficiency of synthetic antioxidants has been recognized, both consumers and manufacturers are looking for more innovative, healthy and quality products while rejecting synthetic additives due to their concern about safety, along with their environmental impact issues. In this context, plant biomass, which have shown to be rich in compounds, have raised interest for the isolation of novel naturally occurring antioxidants. Among their myriad of molecules, bioactive peptides, which are biologically active sequence of amino acid residues of proteins, seem to be of a great interest. Therefore, the number of identified amino acids sequences of bioactive peptides from plant biomass with potential antioxidant action is progressively increasing. Thus, this review provides a description of 129 works that have been made to produce bioactive peptides (hydrolysate, fraction and/or isolate peptide) from 55 plant biomass, along with the procedure to examine their antioxidant capacity (until 2019 included). The protein name, the process, and the method to concentrate or isolate antioxidant bioactive peptides, along with their identification and/or specificity were described. Considering the complex, dynamic and multifactorial physico-chemical mechanisms of the lipid oxidation, an appropriate in-vitro methodology should be better performed to efficiently probe the antioxidant potential of bioactive peptides. Therefore, the results were discussed, and perspective for antioxidant applications of bioactive peptides from plant biomass was argued.

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“…In recent years, a number of peptides with well‐established anticoagulant activity have been isolated from microbial, animal and plant sources . Hydrolyzed protein with anticoagulant activity from amaranth, peanut, pacific salmon, and Mucuna pruriens were obtained using commercial enzymes. In our laboratory, peptide fractions isolated from soluble proteins of goat cheese have also shown in vitro anticoagulant activity…”

Section: Introductionmentioning

confidence: 99%

Enzymatic and chemical synthesis of new anticoagulant peptides

Origone

Bersi

Illanes

et al. 2018

Biotechnology Progress

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In this study we report the enzymatic synthesis of N-α-[Carbobenzyloxy]-Tyr-Gln-Gln (Z-YQQ), a new anticoagulant tripeptide. It was obtained using phytoproteases from the stems and petioles of Asclepias curassavica L. as catalyst in an aqueous-organic biphasic system formed by 50% (v/v) ethyl acetate and 0.1 M Tris-HCl buffer pH 8. The resulting peptide was compared with the analogous peptide Tyr-Gln-Gln (YQQ) produced by solid-phase chemical synthesis. The in vitro anticoagulant activity of the aforementioned peptides was determined using Wiener Lab Test (Wiener, Argentina). The toxicological activity of the peptides was also determined. The enzymatically synthesized Z-YQQ peptide acted on the extrinsic pathway of the coagulation cascade, delaying the conversion time of prothrombin to thrombin and fibrinogen to fibrin by 136 and 50%, respectively, with respect to the controls. The chemically synthesized YQQ peptide acted specifically on the intrinsic pathway of the coagulation cascade, affecting factors VIII, IX, XI, and XII from such cascade, and increasing the coagulation time by 105% with respect to the control. The results suggest that two new anticoagulant peptides (Z-YQQ and YQQ) can be useful for safe pharmaceutical applications. Nevertheless, some aspects related to peptide production should be optimized. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018 © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1093-1101, 2018.

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Antithrombotic and Antioxidant Activity of Amaranth Hydrolysate Obtained by Activation of an Endogenous Protease

Cited by 28 publications

References 35 publications

In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates

In vitro anticancer and antioxidant potential of Amaranthus cruentus protein and its hydrolysates

Production and antioxidant capacity of bioactive peptides from plant biomass to counteract lipid oxidation

Enzymatic and chemical synthesis of new anticoagulant peptides

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